How to Thermostability?

1,3-1,4-Beta-glucanase mutant,VLP Stabilized Vaccine Compositions,Method for expressing and purifying recombinant ethanol oxidase in pichia pastoris

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Thermostability is the quality of a substance to resist irreversible change in its chemical or physical structure, often by resisting decomposition or polymerization, at a high relative temperature. Thermostable materials may be used industrially as fire retardants. A thermostable plastic, an uncommon and unconventional term, is likely to refer to a thermosetting plastic that cannot be reshaped when heated, than to a thermoplastic that can be remelted and recast.

1,3-1,4-Beta-glucanase mutant

ActiveCN104130988AImprove activity stabilityImprove thermal stabilityBacteriaWort preparationMutaseGlucanase

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

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Owner:无锡正元生物科技有限公司

VLP Stabilized Vaccine Compositions

ActiveUS20170065704A1Powder deliveryViral antigen ingredientsKilodaltonThermostability

The invention is directed to compositions and methods for the stabilization of viral and bacterial vaccines. Vaccines of the invention are contained in VLPs with stabilizing agents such as, for example, sugar alcohols (e.g., sorbitol) and degraded gelatins. Preferably the gelatin has an average molecular weight of 10,000 kilodaltons or less. These vaccines have a substantially improved thermostability as well as long term stability. The invention is also directed to the manufacture of a vaccine or the invention and methods for the administration of a vaccine of the invention to patients.

Owner:INVENTPRISE INC

Method for expressing and purifying recombinant ethanol oxidase in pichia pastoris

InactiveCN102533808AKeep activeHigh purityOxidoreductasesFermentationWild typePichia

The invention discloses a method for expressing and purifying recombinant ethanol oxidase rAOX. According to the method, an ethanol oxidase-poly-L-histidine expression vector pPIC9K-AOX-HIS is constructed first by a molecular biological technique, then the expression vector pPIC9K-AOX-HIS is linearized and transferred to pichia pastoris GS115, and thus, pichia pastoris engineering bacteria capable of expressing active recombinant ethanol oxidase are obtained, and part of recombinant ethanol oxidase can be secreted and expressed. Through optimization of the expression conditions of recombinant ethanol oxidase, the ethanol oxidase produced by the engineering bacteria may reach 1,862U / L. Meanwhile, the secretion and expression of the ethanol oxidase and the introduction of a poly-L-histidine tag simplify the purification process of the recombinant ethanol oxidase. According to the result of tests, the recombinant ethanol oxidase expressed by the engineering bacteria has much higher thermostability than pichia pastoris wide type ethanol oxidase. The operation in the whole process of the method is simple, so the method is suitable for industrial production and can create certain economic benefit.

Owner:ZHEJIANG DEQING HUINING BIOTECH

3 results about "Thermostability" patented technology

1,3-1,4-Beta-glucanase mutant

VLP Stabilized Vaccine Compositions

Method for expressing and purifying recombinant ethanol oxidase in pichia pastoris

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