79results about "Biological water/sewage treatment" patented technology

The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.

A method of processing a return oil-based drilling fluid includes centrifuging a primarily fluids phase at a first speed and separating the primarily fluids phase into a first effluent and a first residual, centrifuging the first effluent at a second speed and separating the first effluent into a second effluent and a second residual, and centrifuging the second effluent at a third speed and separating the second effluent into a third effluent and a third residual. A surfactant, a polymer, combinations of surfactant(s) and polymer(s) and/or a wash water may be added to one or more of the return oil-based drilling fluid, the primarily fluids phase, the primarily solids phase, the first effluent, the second effluent, and the third effluent.

The invention discloses an alcaligenes faecalis and application thereof. The alcaligenes faecalis is named alcaligenes faecalis (Alcaligenesfaecalis) NR and is stored in the China General Microbiological Culture Collection Center (CGMCC) with CGMCCNo.7543 on April 28, 2013. The alcaligenes faecalis is applicable to the treatment of nitrogen-containing organic wastewater and can be used for synchronously removing nitrogen and organic carbon in the wastewater. The alcaligenesfaecalis NR has both functions of denitriding and removing the organic carbon synchronously, only needs one aerobic stage to realize the synchronous removal of the organic carbon and nitrogen, and has the removal rate of about 80 to 90% for the organic ratio, and the removal rate of about 78 to 88% for TN (Total Nitrogen).

A wastewater treatment method and apparatus including a denitrification (anoxic) chamber for denitrifying wastewater, an aeration basin with Bio-Wheel for aerating the wastewater, and a membrane device in which sludge particles are separated out of the wastewater, leaving clean discharge water and reusable sludge. Sludge from the membrane chamber floor is sent back to the aeration basin or denitrification chamber or both. Excess sludge is wasted or held for further processing. Preferably, the method and apparatus facilitate both activated sludge and fixed film processes.

The invention discloses a method and apparatus for immobilizing cells to treat wastewater wherein abandoned mycelium is used as a carrying agent for co-immobilization of white-rot fungi and bacteria, the enzyme system in the bacterium group is employed for coupled catalytic degradation of heterocyclic compounds and phenol in the waste water at the presence of oxygen supply. The device includes a reactor with moving sieving board and an oxygen supplying device.

Treated water with good water quality which can be discharged or reused is obtained by efficiently removing SS, fluorine, cyanogen, selenium, ammonia, and COD components contained in coal gasification wastewater such as gas washing wastewater generated in a coal gasification step. A method of treating coal gasification wastewater comprising steps (1) to (4), wherein the step (1) is performed prior to the step (2): (1) a fluorine removal step in which fluorine is removed by coagulation sedimentation; (2) a cyanogen decomposition step in which cyanogen is decomposed by wet oxidation or thermal hydrolysis; (3) a selenium treatment step in which selenate ions are subjected to a reduction treatment with a metal reductant; and (4) a COD/ammonia removal step in which COD and/or ammonia are/is removed.

The invention discloses pseudomonas stutzeri and its culture, immobilization and use. The disclosed pseudomonas stutzeri is pseudomonas stutzeri WZUF25, is preserved in the China general microbiological culture collection center and has the preservation number of CGMCC NO. 7685. The pseudomonas stutzeri has wide aerobic denitrification suitable pH and temperature ranges, has a high NO3<->-N and NO2<->-N removal rate and a high NH4<->-N assimilation capability, and can provide a culture source for synchronous nitrification and denitrification. The invention also provides immobilized pseudomonas stutzeri prepared by an active carbon-calcium alginate embedding method. The immobilized pseudomonas stutzeri has a good NO3<->-N removal capability and good stability.

The invention discloses a method for constructing a multi-stage subsurface flow constructed wetland system, and the method comprises the following steps: (1) selecting a littoral zone with a slope as a wetland pond or digging and backfilling to get a wetland pond with a slope; (2) grading and filling a matrix, namely designing stages according to the pollution load and the height difference of slope, and filling the matrix in each stage of the wetland; (3) arranging drainage holes, water outlet holes and water inlet holes; (4) planting plants, namely planting the plants on each stage of the wetland respectively. The method disclosed by the invention has the following beneficial effects: (1) the system constructed by the method has no influence on the coastal zone structure of a landscape water body. (2) the system constructed by the method has a better purification effect on water flowing into rivers and water contaminated by the landscape water body, especially has an obvious purification effect on pollutants such as SS (Stainless Steel), oil and COD (Chemical Oxygen Demand) and has a good removal effect on heavy metals, TN and TP. (3) the system constructed by the present invention can integrate the purification function with the greening of coastal zones, so that the diversity of landscape is enriched and the overall landscape effect is improved.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The invention belongs to the technical field of microbes, and discloses a composite microbial inoculant for treating coal chemical industry sewage. The composite microbial inoculant is prepared from the following steps: 1) preparing auxiliary materials; 2) preparing paste liquor; 3) preparing a carrier; and 4) preparing the composite microbial inoculant. The composite microbial inoculant can effectively remove ammonia nitrogen, sulfides and phenyl amines in coal chemical industry sewage, has favorable removal effect and achieves the discharge standard.

The invention discloses a bacterial strain for producing laccase, a method for producing laccase by the bacterial strain, the produced laccase and application of the laccase, and relates to the field of microbial enzyme. The bacterial strain for producing laccase is trametes pubescens (Trametes pubescens BJFC-C1108) with a microbial preservation number of CGMCC No. 10308. By utilizing the trametes pubescens (Trametes pubescens BJFC-C1108), the yield of the produced laccase is relatively high; the enzyme activity can be up to 28.687 U/mL; the prepared laccase has relatively high activity, stability and tolerance under the alkali condition (pH 7.0-10.0), has relatively high tolerance for metal ions, can be used for shortening the time required by complete decoloring of azo dye Congo red to a certain extent, has a potential application value, can be applied to more industrial fields and has favorable development and application prospects.

The present invention relates to a purification processing system of the slightly polluted water, especially an earth purification processing system used for processing the landscape water body. Firstly the groove is dig on the earth and the anti-seepage processing is executed to the inner side of the groove, the inner side of the groove is filled with filling agent and the initial two ends of the groove are respectively arranged with a water inlet pipe and a water outlet pipe, the upper layer of the filling agent is covered with the water-permeating material and the upmost layer is laid with natural soil; the water to be processed is inputted into the groove from the water inlet pipe and enters the filling agent, at the same time because of the capillarity of the water-permeating material and the natural soil the water to be processed is suck into the filling agent layer of the soil, and the water passing through the synthetic function of the filling agent, the soil, the plant and the microorganism becomes purified water. Comparing with the present technology the method has the advantages of effectively processing the slightly polluted landscape water on the spot, practicality, easiness, not affecting the surrounding landscape in the actualizing method of the method, adopting the pure ecological technique and not generating the secondary polluting matter.

The invention relates to a method for removing bromate in water through oxidation reduction and a system thereof. The method comprises the following steps: firstly, controlling the dissolved oxygen concentration in the water at a lower level through the nitrification of ammonia salt and microbes on the active carbon; secondly, removing the bromate in the water through the reducibility of ferrous sulfate; and finally, removing excessive ferric ions in the water through a filtering unit. The system comprises a pretreatment working section for controlling the dissolved oxygen in the water, an oxidation reduction working section for removing the bromate and a filtering treatment working section for removing the excessive ferric ions, wherein the pretreatment working section is connected with the oxidation reduction working section, and the oxidation reduction working section is connected with the filtering treatment working section. The method can effectively remove the bromate in the water with a bromate removal rate of above 60 percent. The system has convenient operation and low running cost, can also be integrated with other technologies, and have very high practicability.

The invention belongs to the technical field of industrial water treatment, and particularly relates to a method for removing calcium carbonate scales by using microbial extracellular carbonic anhydrase. The invention aims to provide a method for relieving the phenomenon of carbonate scales in an industrial circulating water system. The method can reduce the phosphorus-containing wastewater which enters the ambient water body. The technical scheme is as follows: the method comprises the following steps: 1) preparing a bacillus microbial inoculant for secreting extracellular carbonic anhydrase; 2) carrying out refrigerated centrifugation, and salting out to extract a crude enzyme solution containing the carbonic anhydrase; and 3) adding the crude enzyme solution into industrial circulating water with carbonate scales. The method can be used for removing the calcium carbonate scales in the industrial circulating water system, and has the advantages of low cost and no environment pollution.

The invention discloses a magnetic bioreaction separation device for treating organic wastewater, which comprises a magnetic bioreactor and a rare-earth magnetic disc separation device, wherein the magnetic bioreactor consists of a reaction vessel, and microbe carrier magnetic species or magnetic zooglea formed by the microbe carrier magnetic species, which is placed in the reaction vessel; the magnetic bioreactor is connected with the rare-earth magnetic disc separation device by a flowing discharge pipeline; and the upper part of the rare-earth magnetic disc separation device is provided with a water outlet pipe, and the lower part thereof is provided with a sludge discharge valve. Since the invention utilizes the microbes attached to the microbe carrier magnetic species or magnetic zooglea to decompose the organic matter, while the magnetic zooglea and the treated water can be treated by the rare-earth magnetic disc separation device for solid-liquid separation, not only the cost on the investment and operation is lower than that of the membrane bioreactor, but also the separation efficiency is high, the energy consumption is low, and the effect on denitrification, phosphorous, SS, COD and BOD removal; and the invention is particularly suitable for treating the organic wastewater with large flow and high concentration.

The invention discloses a compound microbial agent for treating dye waste water as well as preparation and application of the compound microbial agent. The compound microbial agent is formed by complexing acinetobacter haemolyticus, Escherichia coli, bacillus flexus, Yersinia intermedia, luther enterobacter and Klebsiella pneumoniae. Complexing is simple and convenient, the broad spectrum is strong, the difficulty that a single microbial agent can not meet the requirement of water quality variation is overcome, and the cost of treating the dye industrial waste water is reduced.

The invention discloses an efficient liquid sodium acetate carbon source for degrading ammonia nitrogen in wastewater. The efficient liquid sodium acetate carbon source is prepared from sodium acetate, glacial acetic acid, sodium carbonate, ethyl alcohol, glucose and deionized water. The efficient liquid sodium acetate carbon source is prepared through the following steps: uniformly mixing sodiumacetate and glacial acetic acid, adding a part of deionized water, stirring to obtain a solution 1; taking sodium carbonate, adding a part of deionized water, and stirring to obtain a solution 2; taking glucose, adding a part of deionized water, and stirring to obtain a solution 3; and mixing the solution 1, the solution 2 and the solution 3, sequentially adding ethyl alcohol and the rest of deionized water, and uniformly stirring to obtain the efficient carbon source (liquid sodium acetate) for degrading ammonia nitrogen in wastewater. The efficient liquid sodium acetate carbon source has thebeneficial effects that sodium acetate is harmless to a human body, is low in cost, is easily absorbed and used by microorganisms, and is an ideal external carbon source; glacial acetic acid can externally increase a carbon source, and the pH value is maintained in the sewage treatment process; and sodium carbonate can provide an inorganic carbon source for a part of microorganisms.

The invention provides a biological treatment method of beer wastewater, and the method utilizes the activity of biological enzyme and metabolism of hydrolysis acidate bacteria to treat harmful substances in the beer wastewater. The method has the advantages of simple operation, short treatment time, having no secondary pollutant in the treatment process, safety and environmental protection.

The invention discloses a discharge treatment system for the produced water of a polymer flooding oilfield, and belongs to biochemical discharge treatment of tertiary oil recovery polymer-containing sewage of the oilfield. Sulfate reducing bacteria having polyacrylamide degradation ability are cultured and screened; the produced water of the polymer flooding oilfield is treated though a combined process comprising a sulfate reducing bacteria pretreatment unit, a hydrolytic acidification unit and a contact oxidation unit; the sulfate reducing bacteria pretreatment unit is used for partially degrading and removing residual HPAM (Hydrolyzed Polyacryamide) out of the sewage at first, so that the load of the subsequent treatment units is reduced; a low-intensity ultrasonic generator is used for increasing the activity of a microbial enzyme by performing irradiation treatment on the activated sludge so that the capacity of decomposition of microorganisms on organic matters is improved; several techniques are combined to achieve the synergistic effect for improving the overall treatment capacity and the applicability of the process to the produced water of the polymer flooding oilfield. The discharge treatment system provided by the invention is simple in process and easy to implement, and is expected to realize standard discharge of the produced water of the polymer flooding oilfield in combination with auxiliary units such as a sedimentation tank.

The invention provides high-efficiency sulfate reducing bacteria (the bacteria is named Desulfotomaculum halophilum RETECH-SRB-II, the preservation unit is China Center for Type Culture Collection in Wuhan University, the preservation date is May 11th, 2007, and the preservation registration number is CCTCC NO of M207061) with wider growth temperature and pH applicability; the invention simultaneously provides a technique that can treat acid mine wastewater with the carbon and nitrogen source of straws. In the treating of the acid mine wastewater, the technique reaches the Cu<2+> removing rate of 89 to 97 percent, the Zn<2+> removing rate of 90 to 98 percent, the Fe<2+> removing rate of 91 to 99 percent, the Fe<3+> removing rate of 79 to 91 percent, and the As<3+> removing rate of 80 to 90 percent. The treated wastewater takes neutral pH value. The technique has the advantages that straws can be used as the carbon and nitrogen source required for culture growth, thus lowering the bacteria cultivation cost by 30 to 50 percent.

The invention provides a biologic slow release ball used for governing black and odorous rivers. The ball is prepared from, by weight, 40-60 parts of zeolite powder, 40-60 parts of composite bacteria,20-30 parts of glutinous rice soup and 0.4-1 part of brown sugar. The biologic slow release ball used for governing the black and odorous rivers can slowly release the composite bacteria and substantially increase the utilization rate of the bacteria in comparison with a mode of directly putting the bacteria. The biologic slow release ball can effectively prevent the bacteria in the ball from being damaged by adverse environmental factors of water and gradually have the capability of adapting to a bad water environment. Nutrients capable of taking effect quickly or slowly and microenvironmental physical and chemical factor adjusting substances are contained in the biologic slow release ball, the biologic slow release ball can be utilized in a constant release process of the bacteria, andmass propagation of the bacteria is achieved.