4 results about "Artificial culture" patented technology

The invention discloses a smelt artificial propagation method. From the early June, the fodder containing more fish meal protein is used for the feeding of female parent fishes. While from the middle of September, the female parent fishes are fed on the fodder containing spirulina powder and aluzyme. After the parent fishes enter the gonad mature period, the better mature female parent fishes are selected and cultivated after the estrualization, and the sexually mature male parent fishes are selected and cultivated after the estrualization. After the cultivated parent fishes reach more than 60 % gonad maturity, the water temperature of a decorporation pool is dropped continuously for 2 days with the decline of 2 degree each day. Afterwards the constant temperature is kept for 4 to 6 days. During the ovulatory period of parent fish, the ovums and the sperms are artificially collected every two days. The dry artificial insemination method is used to obtain a germ cell, and the germ cell is put in a hatchery pond for breeding. With the invention, the smelt sverage absolute fecundity and the fertility rate can be enhanced, and a great number of fry can be obtained in each breeding. The scale of artificial propagation is increased, and the labour intensity of the artificial propagation is reduced. Thus a foundation for the large scale artificial culture of smelt is established.

The invention belongs to the field of medical detection instruments and particularly relates to a mycoplasma culture plate automatic interpreter. The mycoplasma culture plate automatic interpreter comprises a culture assembly, an analysis assembly, a moving assembly, a main controller, a display screen, a printer and a shell carrying the above assemblies. For the mycoplasma culture plate automaticinterpreter provided by the invention, by arranging the culture assembly, the analysis assembly, the moving component, the main controller, the display screen and the printer, operation of artificialculture and manual interpretation in traditional operation is replaced, the working efficiency is greatly improved, and automatic operation of mycoplasma identification and drug sensitivity analysisis achieved.

The invention relates to the technical field of biological 3D printing, in particular to a method for facilitating in-vitro maturation of human immature oocytes by utilizing a 3D printing technology. The method comprises the following steps of (1) properly treating naked ova and granular cells of ovary cells; (2) performing in-vitro balanced culture of ovarian granular cells; (3) putting oocytes into the ovarian granular cells, and continuing to perform culture for 2 days; (4) preparing 3D printing ink; (5) preparing 3D printing gel; and (6) printing out follicles by adopting the 3D printing technology, putting the oocytes and an in-vitro maturation culture solution into the follicles, and performing culture for one week. According to the method, the follicles with a biochemical environment similar to that of a human body try to be simulated, and then the oocytes are quickly and accurately put into the follicles for performing artificial culture by depending on the 3D printing technology, so that the in-vitro maturation of the human immature oocytes is realized; the method depends on the 3D printing technology; and the whole process is quick and convenient, and the automation degree is high, so that the technical expense of the in-vitro maturation of the oocytes can be reduced.