74results about "Biochemistry cleaning apparatus" patented technology

Methods and compositions are provided for the identification of expression signatures in ER+ breast cancer cases, where the signatures correlate with responsiveness, or lack thereof, to tamoxifen treatment. The signature profiles are identified based upon sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for predicting the efficacy of treating a subject with ER+ breast cancer with tamoxifen. Additional methods and compositions are provided for predicting tamoxifen responsiveness in cases of ER+ breast cancer by use of three biomarkers. Two biomarkers display increased expression correlated with tamoxifen response while the third biomarker displays decreased expression correlated with tamoxifen response.

In on aspect, the invention includes a microcarrier bead having a porous three-dimensional core having (a) a polymeric porous three-dimensional body having porosity of about 15 to about 90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns, (b) an outer protective layer and optionally (c) a filler. In another aspect, the invention includes a method of making an artificial scaffold wherein a scaffolding material is extruded into a coolant and thereby creating a porous material having a porosity of between 15-90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns.

The invention provides methods and systems for detecting and measuring microRNAs.

The present invention provides, in part, methods for recombinantly expressing proteins at a high level along with cell culture media for doing the same.

A membrane supported bioreactor arrangement and method for anaerobic conversion of gas into liquid products including membrane modules having hollow fibers packed across a cross sectional area of the membrane module, each of the hollow fibers formed from an asymmetric membrane wall having a porous outer layer defining biopores for retaining a porous biolayer about the outer surface of the membrane wall and a less permeable hydration layer around the hollow fiber lumen; a membrane vessel for surrounding the outside of the hollow fibers with a process gas from a gas supply conduit; and a liquid supply conduit operably connected to the hollow fibers for supplying a process liquid to the hollow fiber lumens. The gas supply conduit enables the formation of a biolayer on the outer surface of the hollow fiber wall by interaction of microorganisms with the process gas and the production of a liquid product.

Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

Methods and systems for the production of hydrocarbon products, including providing a substrate comprising CO to a bioreactor containing a culture of one or more micro-organisms; and fermenting the culture in the bioreactor to produce one or more hydrocarbon products. The substrate comprising CO is derived from a CO2 reforming process.

The invention belongs to the field of tools for in-situ colonization and culture systems for deep-sea microorganisms and in particular relates to a culture cabin for deep-sea microorganisms. According to the technical scheme, the uncovered culture cabin for deep-sea microorganisms comprises linear bearings (1), tension springs (2), a pressure compensation cavity (3), a fixed top plate (4), a deep-sea motor component (5), a fixed bottom plate (6), a hose (7) and a culture cabin body (8). The uncovered culture cabin has the beneficial effects that an end cover of the culture cabin body (8) is pushed away through rotation of the deep-sea motor component (5), microorganism enrichment culture is carried out in a completely open state and the end cover of the culture cabin body (8) is closed in laying and recovery processes to achieve sealing of the microorganism culture cabin body; the uncovered culture cabin can achieve microorganism enrichment culture in a deep-sea in-situ state; the safety during sample recovery can be ensured and the weight of the complete set of system can be effectively reduced by adopting the pressure compensation cavity (3).

The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels.

The invention aims to provide a microfluidic chip system for culture and multiplication behavior research of marine microalgae. Inoculation, batch culture, semi-continuous chemostat culture of the marine microalgae and a marine microalgae population multiplication behavior online monitoring process are integrated on a functional chip to be completed by means of fabrication of a scale integration chip and flexible combination of multiple cell technologies on the chip. The microfluidic chip system consists of two elementary units, wherein the first elementary unit refers to the microfluidic chip for culture and multiplication behavior research of the marine microalgae, and the second elementary unit refers to microfluidic chip peripheral equipment for culture and multiplication behavior research of the marine microalgae. The microfluidic chip system has the advantages that the microfluidic chip system is high in throughput, simple and convenient to operate and low in cost and is a novel platform for marine microalgae culture, population multiplication research and correlational research fields of using microalgae as experimental subjects.

In one aspect, the present disclosure provides a method for separating a target component in a fluid sample, which method comprises: a) passing a fluid sample that comprises or is suspected of comprising a target component and cell aggregates through a microfabricated filter so that said target component, if present in said fluid sample, is retained by or passes through said microfabricated filter, and b) prior to and/or concurrently with passing said fluid sample through said microfabricated filter, contacting said fluid sample with an emulsifying agent to reduce or remove said cell aggregates, if present in said fluid sample.

Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass frit.

The present invention relates to in vitro methods for determining efficacy of topical compositions on various skin color types. These methods involve providing an artificial skin apparatus configured to approximate the color and diffuse reflectance characteristics of a predetermined human skin color type. The artificial skin apparatus includes an artificial skin substrate combined with a color background, with the color background correlating to the human skin color type. A topical composition of interest is applied to the artificial skin substrate of the artificial skin apparatus and then pre-irradiated. The pre-irradiated topical composition is then analyzed using relevant experimental techniques or assays for at least one efficacy parameter, including, for example, anti-ageing, photoprotective, sun protective, UVA/UVB protective, UVAI protective, photo stabilizing, and photosensitizing activities and action mechanisms of the topical composition on various skin color types. Also provided is an artificial skin apparatus and kit for use with the in vitro method.

A method for controlling electrical power consumption for a first group of functional which can be used for operational management during operation of the bioreactor components during operation comprises, inter alia, adjusting a present power control signal for one or more of the functional components from the first group in order to optimise power consumption, when a comparison shows for the first group of functional components that the currently required total electrical power consumption is greater than a predefined total electrical power consumption, such that, for the first group of functional components, an adjusted total electrical power consumption is not greater than the predefined total electrical power consumption. A biotechnological apparatus comprises a bioreactor, a reactor vessel formed in the bioreactor and having a cultivation chamber, and a temperature control device provided with a heat pump and configured to control the temperature of the cultivation chamber.

The invention provides a depressive disorder biomarker. The depressive disorder biomarker comprises Clostridium saccharolyticum and Heliobacterium modesticaldum, and also comprises Capnocytophaga, Saccharomonospora viridis, Helicobacter felis, Ruminococcus, Acidaminococcus Rogosa, Acidaminococcus and Acidaminococcus fermentans. The invention further provides a kit for diagnosis of depressive disorder, a composition for preventing or treating depressive disorder and a system for screening a biological sample susceptible to depressive disorder. The system can screen a biological sample susceptible to depressive disorder. The invention further provides a screening method of the biomarker for diagnosis of depressive disorder. The method can screen the biomarker for diagnosis of depressive disorder.

A simple and accurate separation purification method for object biomolecules or bio-associated substance through a particulate aggregative reaction is provided. A microfluidic circuit utilizing a particulate aggregate as a detection marker in a nonfluorescence method such as an electrochemical method or surface plasmon resonance is provided. The separation purification method comprises the steps of forming a particulate aggregate by a reaction between particulates modified with a labeling substance specifically reacting with a label object substance and biomolecules or a bio-associated substance containing the label object substance and separation-purifying the particulate aggregate. The microfluidic circuit utilizes this separation purification method.

Fibrous protein fibers such as keratin fibers can be used to detect chemicals and radiation. One aspect of the invention is a method of detecting an analyte in a sample comprising the steps of: (1) providing a fiber of fibrous protein; (2) contacting the fiber of fibrous protein with an sample that may contain an analyte; (3) measuring the conductivity of the fiber of fibrous protein in the absence of contact with the sample and in the presence of contact with the sample; and (4) correlating the conductivity of the fiber of fibrous protein in the presence of contact with the sample with the conductivity of the fiber of fibrous protein in the presence of contact with a reference sample containing a known concentration of analyte to detect or determine the analyte in the sample. Another aspect of the invention is a method of detecting radiation in a sample using a fibrous protein fiber functionalized with CdTe.

The embodiment of the invention provides a biogas fermentation gas supply engineering control system, comprising a master control sub-system, a programmable logic controller (PLC) control sub-system, a local control sub-system and a controlled sub-system, wherein the PLC control sub-system is respectively connected with the master control sub-system and the local control sub-system; the controlled sub-system is connected with the local control sub-system; the controlled sub-system comprises a material collecting unit, a degritting blending unit, a biogas generating unit, an air storage unit, a mud-water separating unit and a biogas power generation unit; the master control sub-system is used for automatically controlling the controlled sub-system through the PLC control sub-system; and the local control sub-system is used for switching the controlled sub-system between a local manual control state and a remote control state. By adopting the system, the instantaneity, the stability and the reliability of a biogas fermentation gas supply system are significantly improved; information management is facilitated; the operating cost is reduced; the working efficiency is improved; an accident is prevented; and the biogas fermentation gas supply engineering control system has high practical application value.

This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.

Disclosed is a method for calculating a ratio of glycosylated hemoglobin with high accuracy by electrochemically detecting the concentration of fructosyl valine or fructosyl valyl-histidine in a sample. Also disclosed is an apparatus for assaying glucose and glycosylated hemoglobin simultaneously. Further disclosed are a method and an apparatus for removing hydrogen peroxide in a sample.

The invention concerns a microfluidic system (1) comprising:

• • at least one channel (2) for the flow of fluid having an inlet (4), an outlet (5) and a longitudinal axis (7) extending between the inlet (4) and the outlet (5), said channel (2) comprising a capture zone (3), and the cross section of the channel (2) orthogonal to the longitudinal axis (7) of the channel (2) increasing in size in the capture zone (3), from the inlet (4) towards the outlet (5) of the channel (2); and
  • means (6) for applying a magnetic field having a decreasing intensity in the capture zone (3) of the channel (2), from the inlet (4) towards the outlet (5) of the channel.

The invention also concerns a method for treating a sample that can be implemented with this system.

The present invention relates to apparatus, systems and methods for generating a subpopulation of spermatozoa enriched for capacitated spermatozoa, by exposure of a population of spermatozoa to a suitable temperature gradient, and retrieving the enriched subpopulation of spermatozoa for further applications, such as, for diagnosis or fertility treatments.

The invention discloses an intelligent biogas material reaction box, which comprises a pool body. A conveyor belt is arranged at the bottom of the pool body. The conveyor belt is driven by rollers. The surface of the conveyor belt is provided with a plurality of baffles. The operating port is equipped with a sealing cover. The intelligent biogas material reaction box of the present invention is controlled by a single-chip microcomputer, combined with sensor technology, to realize the intelligent operation control of the entire device, which can automatically judge the reaction of the material. The reaction parameters intelligently control the operation of each electrical component, and the reaction in this design is carried out in the liquid. In order to speed up the reaction efficiency, rotating blades and scrapers are added, which can not only make the upper and lower liquids move with each other, but also make the inner wall of the box The reactants will not stick to the surface, and the sundries after the reaction are automatically removed through the conveyor belt and the lifting motor, which has the advantages of saving manpower, high intelligence and simple operation.