Microfluidic chip system for culture and multiplication behavior research of marine microalgae

[0027] Using the microfluidic chip system designed and produced by the laboratory, the configuration is as follows: figure 1 and figure 2 Shown, inoculate microalgae, present embodiment selects subcardiac, Chlorophyta, common dominant species in China's coast, inoculation density is at 10 4 above cell / mL. The culture method is batch culture, that is, after one-time addition of the culture medium, the nutrient components will not be replaced during the culture period, and the culture period is 9 days. The culture medium adopts the formula of f / 2 nutrient solution, the culture conditions are temperature 20℃±1℃, light-dark cycle 12h:12h, the light source is white fluorescent lamp, and the illumination is 60 μmol photon m -2 .s -1 . Every 24 hours, the microalgae in the chip culture room were photographed under the microscope. In order to increase the identification of the microalgae, the laser-induced fluorescence mode was used, and the Image Pro software was used for cell

[0029] Using the microfluidic chip system designed and produced by the laboratory, the configuration is as follows: figure 1 and figure 2 As shown, microalgae are inoculated, and the present embodiment selects Selmaphytum Qingdao, Chlorophyta, common dominant species in China's coastal areas, and the inoculation density is at 10 4 Cell / mL or more, culture in batches, and the culture period is 9 days. The culture medium adopts the formula of f / 2 nutrient solution, the culture conditions are temperature 20℃±1℃, light-dark cycle 12h:12h, the light source is white fluorescent lamp, and the illumination is 60 μmol photon m -2 .s -1 . Every 24 hours, the microalgae in the chip culture room were photographed under the microscope. In order to increase the identification of the microalgae, the laser-induced fluorescence mode was used, and the Image Pro software was used for cell counting analysis, and the population proliferation behavior was observed online. The result is as F

[0031] Using the microfluidic chip system designed and produced by the laboratory, the configuration is as follows: figure 1 and figure 2 Shown, inoculate microalgae, present embodiment selects Phaeodactylum tricornutum for use, diatom door representative species, inoculation density is at 10 4Cell / mL or more, culture in batches, and the culture period is 9 days. The culture medium adopts the formula of f / 2 nutrient solution, the culture conditions are temperature 20℃±1℃, light-dark cycle 12h:12h, the light source is white fluorescent lamp, and the illumination is 60 μmol photon m -2 .s -1 . Every 24 hours, the microalgae in the chip culture room were photographed under the microscope. In order to increase the identification of the microalgae, the laser-induced fluorescence mode was used, and the Image Pro software was used for cell counting analysis, and the population proliferation behavior was observed online. The result is as Figure 5 As shown, the cell appearance