Methods for cultivating cells and propagating viruses

a technology for propagating viruses and cultivating cells, applied in the field of cultivating cells and propagating viruses, can solve the problems of severe problems in scaling up the cultivation other cell lines do not grow well without a support, and the growth of a cell line that requires a support is often limited

Inactive Publication Date: 2005-01-27
SCHERING CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present technology relates to methods used during cell culture processes that involve suspending tiny particles called microspheres or beads inside small containers made up of plastic material like polystyrene foam. These minute objects can help keep other organisms away from them while they are being cultured on these carriers. This helps improve their growth rate compared to traditional suspension techniques where only one type of substance remains fixed within each container.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the method of growing large amounts of genetic material from small isolated colonies of bacteria grown alone onto surfaces such as plastic tubings or glass flasks while also preventing cloning during scaleup due to lack of space at their edges.

Method used

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  • Methods for cultivating cells and propagating viruses
  • Methods for cultivating cells and propagating viruses

Examples

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experimental examples

I. Overview

A. Cell Inoculum Preparation

Each viral fermentation batch is started from a cell line propagated from a vial of 293 cell Manufacturers Working Cell Bank (MWCB). The bioreactors are inoculated with 293 cells (ATCC catalog number CRL 1573) maintained by propagation in T-flasks and CELL FACTORY™ using growth medium (Medium 1), as illustrated in Table 1 below. Each transfer represents a passage. Typically, passage numbers of 4 to 30 are employed for inoculation of a seed bioreactor.

TABLE 1MEDIA COMPOSITIONTypicalComponentTypicalComponentMediumPurposeFunctionComponentRangeMedium 1CellCell GrowthDMEM Powder110-20g / l(GrowthGrowthCell GrowthGlutamine0.1-1.0g / lmedium)Cell GrowthBovine Calf5-15%Serum3BufferSodium2-4g / lBicarbonateMedium 2Prepara-Reduce Ca++DMEM Powder,10-20g / l(Serum-tion forfor tryp-Ca++freefreetrypsin-sinization,Glutamine0.1-1.0g / lwashizationLater washSodium2-4g / lmedium)EDTABicarbonateCellViabilityBufferMedium 3Prepara-Reduce Ca++DMEM Powder,10-20g /

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Abstract

This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.

Description

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Claims

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Application Information

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Owner SCHERING CORP
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