34 results about "Glucose polymers" patented technology

Devices and methods for determining analyte levels are described. The devices and methods allow for the implantation of analyte-monitoring devices, such as glucose monitoring devices, that result in the delivery of a dependable flow of blood to deliver sample to the implanted device. The devices comprise a unique microarchitectural arrangement in the sensor region that allows accurate data to be obtained over long periods of time.

Disclosed is a process for extracting xylose and xylitol from a xylose mother liquor or a xylose digest, which comprises, using xylose hydrolysate or xylose mother liquid as the raw material, removing glucose through saccharomyces cerevisiae fermentation, then imitating moving bed, using water as eluent, completely separating xylose from foreign matter such as arabinose, thus obtaining component containing rich xylose.

It is an object of the present invention to provide a solution for creating an effective method for retarding unhealthy manifestations brought by ageing of human beings (in particular, but not limited to the reduction of sexual activity and fertility, climax, changes in glucose tolerance, reduction of cognitive and mnestic functions, reduction of stress resistance, development of organ and tissue sclerosis) without directly affecting the genetic apparatus of the ageing cells.

According to the invention this task is solved by administration into the blood circulation of the agent which inactivates extracellular blood plasma DNA; the extracellular blood DNA inactivating agent can be embodied in the form of an extracellular blood plasma DNA destroying agent; said extracellular blood plasma DNA destroying agent can be embodied in the form of an DNase enzyme; the extracellular blood plasma DNA inactivating agent can also be embodied in the form of an extracellular blood plasma DNA binding agent; the extracellular blood plasma DNA binding agent can be embodied in the form of anti-DNA antibodies; the extracellular blood plasma DNA inactivating agent can be administered in the form of an enzyme modifying the chemical composition of extracellular blood plasma DNA; the extracellular blood plasma DNA inactivating agent can be embodied in the form of an agent that stimulates synthesis or activity of endogenous deoxyribonuclease, or an agent that stimulates the synthesis of antibodies which capable to bind extracellular blood plasma DNA.

The invention provides a CDs-Pt nanomaterial with catalase catalytic properties. The CDs-Pt nanomaterial shows good enzyme-like catalytic activity in an acetate buffer solution using TMB as a substrate. The CDs-Pt nanomaterial provided by the invention belongs to the field of catalytic material and mimic enzyme research, and the obtained CDs-Pt nanomaterial has the characteristics that carbon quantum dots are modified by precious metal platinum, the synthetic steps are simple, the material scale is small, and the CDs-Pt nanomaterial has good dispersion in a reaction system, and has broad prospect in actual application such as hydrogen peroxide detection and glucose detection.

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention discloses an establishment method for a type 2 diabetes mellitus animal model. The establishment method comprises the following steps: (1) preparing a lipopolysaccharide solution with aconcentration of 100ug/ml by virtue of normal saline; (2) selecting male rats which are 4-6 weeks old, feeding by virtue of a common feed, and simultaneously injecting the lipopolysaccharide solution;(3) weighing the male rats after 28 days; (4) after the weighing, fasting for 8 hours, separating serum, and measuring blood glucose and insulin; (5) evaluating the resistance of the insulin and glucose stimulated insulin secretion capacity; (6) respectively intraperitoneally injecting an STZ solution into the male rats in the 30th day and the 32th day; and (7) determining that the establishmentof the type 2 diabetes mellitus animal model is finished when the random blood sugar is more than 16.8mmol/L. According to the establishment method, lipopolysaccharide in Escherichia coli 0111:B4 subspecies is selected and is matched with the feeding of the common feed and the STZ injection so as to successfully establish the type 2 diabetes mellitus animal mode; according to the establishment method, the operation is easy, the model establishment cost is low, the model establishment rate is high, and the model establishment period is short.

The invention relates to a method for preparing composite yeast nutritious drinks, and belongs to the technical field of nutritious drinks. According to the technical scheme, the method includes the steps that lyceum barbarum extract is prepared; a yeast extract solution is prepared; syrup feed liquid is prepared; the obtained lyceum barbarum extract and the yeast extract solution are added to the syrup feed liquid, the lyceum barbarum extract, the yeast extract solution and the liquid are evenly mixed, sodium erythorbate is added to the mixed solution, and high pressure homogenization is conducted on the mixed solution; the solution after high pressure homogenization processing is sterilized, filtered and clarified through a ceramic membrane, and filled into vessels, and then the composite yeast nutritious drinks are prepared. The prepared lyceum barbarum and yeast nutritious drinks have quite rich nutrient substances, lyceum barbarum contains a lot of nutrients such as amino acids, lyceum barbarum polysaccharides, inorganic salt, glucose, taurine and betaine, and the nutrients are essential to human body; yeast extract contains 18 kinds of amino acids and polypeptides, multiple B vitamins, nucleic acids and multiple mineral elements. The prepared drinks have the functions of nourishing the kidney and tonifying the liver, benefiting qi and promoting the production of body fluid, improving eyesight and soothing nerves, resisting fatigue and improving human body immunity.

The invention discloses a mixed-grain total-nutrition formulation food for patients with inflammatory bowel diseases, and relates to the technical field of healthcare foods. The food is prepared by mixing millet, casein phosphopeptides, protolysate powder, lactalbumin powder, traditional Chinese medicine extraction liquid, glucose, honey and medium-chain triglyceride. Basic nutritional substances, enhanced nutritional elements and Chinese herbal medicinal ingredients are reasonably mixed, generate a synergistic effect in a certain formulation range and have good auxiliary treatment and recovery effects for the inflammatory bowel diseases. The food is good in mouth feel, good in effect, convenient to eat and suitable for auxiliary treatment of the patients with the inflammatory bowel diseases.

Disclosed is a method for calculating a ratio of glycosylated hemoglobin with high accuracy by electrochemically detecting the concentration of fructosyl valine or fructosyl valyl-histidine in a sample. Also disclosed is an apparatus for assaying glucose and glycosylated hemoglobin simultaneously. Further disclosed are a method and an apparatus for removing hydrogen peroxide in a sample.

The invention discloses a preparation method of a paper-based photocathode biosensor for detecting microRNA. A gold nano-particle layer is coated in a working area of a paper-based carbon working electrode; polyhedral cuprous oxide is electrodeposited and lots of silver sulfide quantum dots can be loaded, and a photoelectric sensitized structure is formed, so that the cathode photocurrent is increased substantially; and in the presence of the target microRNA, a double-strand-specific-nuclease-induced target cycle reaction and a cascaded stem-branch hybridization strand reaction are made to obtain a negatively charged dendritic DNA double-helix structure. After assembling of positively charged gold nanoparticles, a gold tree with the good glucose oxidase mimicking activity is formed, so that the glucose oxidation reaction is catalyzed, the dissolved oxygen of the electron acceptor is consumed, the cathode photocurrent signal is reduced, and sensitive detection of the microRNA is realized.

The invention belongs to the field of wine brewing, in particular relates to a brewing method, and more particularly relates to a brewing method of agaricus bisporus and chenopodium quinata wine. According to the brewing method, agaricus bisporus and chenopodium quinata are used as raw materials, novel wine is prepared through fermentation, and the efficacies of reducing blood glucose, lowering blood pressure, reducing blood fat, resisting oxidation, resisting cancer, improving body immunity, relieving cough and reducing sputum, inducing diuresis and relaxing the bowels, expelling toxins and losing weight, and the like are enhanced; due to the adoption of the extrusion puffing method for pretreating chenopodium quinate, the utilization rate of chenopodium quinata is increased, and the costis reduced; the agaricus bisporus and chenopodium quinata wine contains rich nutritional ingredients including amino acids, unsaturated fatty acids, vitamins, minerals and polysaccharides, is convenient to use, is pure and soft in taste, is suitable for wide consumers, and has the broad market and development prospects.

The invention discloses a preparation method of a non-woven fabric, and belongs to the field of spinning. Sodium pyrophosphate, glucose, sodium hydroxide, ferrous sulfate and water are sampled, nitrogen protection is adopted, heating and stirring are performed, and then cumyl hydroperoxide and polybutadiene are added and stirred and mixed; a stirring mixture, allyl benzene, methyl methacrylate, epoxypropyl methacrylate and dibenzyl phthalate are obtained to be added into magnesium sulfate, stirring, mixing and drying are performed, a drying object is obtained to be added into polylactic acid to be mixed, and a mixed matrix is obtained; folium eriohotryae and aloe are obtained to be mixed and homogenized, deionized water is added for digestion and suction filtration, a filtrate is obtainedfor centrifuging, a supernate is obtained to be added into a copper nitrate solution to be mixed, and an antibiotic finish solution is obtained; processed linen fiber, a dispersing solution and the antibiotic finish solution are obtained to be soaked and filtered, the linen fiber is taken to be cleaned with deionized water, and linen fiber is obtained; linen fiber, the mixed matrix, polypropyleneand sodium alginate are obtained to be mixed for opening, carding and lapping, the mixture is placed into a needle machine for needling treatment, and hot rolling is performed to obtain the non-wovenfabric.

The invention discloses an engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its application, specifically a special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase and its use in the production of acetoacetate. The engineering bacterium is obtained by leading the expression vector including the beta-keto thiolase and 3-ketoacid coenzyme A transferase in the Escherichia coli. The present invention also provides a preparation method of acetoacetate, which includes: fermenting the engineering bacterium expressing the beta-keto thiolase and 3-ketoacid coenzyme A transferase to obtain the beta-keto thiolase and 3-ketoacid coenzyme A transferase, and then adding substrate glucose and succinate solution, fermenting to obtain the acetoacetate. The special-purpose expression engineering bacterium of beta-keto thiolase and 3-ketoacid coenzyme A transferase of the present invention will play an important role in the industrialization production and wide application prospects.

The invention provides a solution for evaluating the glucose metabolism based on the sialology method and an evaluation method thereof. The solution comprises the following components: 60-150 mM of pH 7.6 Tris-HCl, 0.12-0.4% of blue chromogenic substances; 1200-2500 IU/L of glucose oxidase; 120-250 IU/L of horse radish peroxidase; and 600-1500 IU/L of antioxidant. The evaluation method comprises the steps of 1) collecting the saliva; 2) mixing the saliva with the above solution; 3) determining the level of glucose metabolism of an organism according to the color depth of the mixed solution of the saliva and the solution. According to the technical scheme of the invention, the level of glucose metabolism is evaluated by adopting the above solution that can change in color depth after reacting with the saliva. In this way, the inconvenience caused by the detection of the blood glucose can be solved. Meanwhile, the evaluation method is visual, convenient, fast, high in efficiency and high in accuracy. At the same time, the antioxidant is added into the above solution, so that the storage life of the solution is prolonged. Moreover, the waste is avoided and the cost is saved.

The invention relates to a selective separation medium for caprine mycoplasmal pneumonia subspecies and a preparation method of the selective separation medium, and belongs to the technical field of veterinary biology. The selective separation medium is prepared from components as follows: lactoalbumin hydrolysate, yeast extract powder, glucose, an MEM medium, a PPLO (pleuropneumonia-like organism) gravy powder, sodium pyruvate, a 1% phenol red solution, specific growth inhibition serum and deionized water. The preparation method comprises steps as follows: the lactoalbumin hydrolysate, the yeast extract powder, glucose, the MEM medium, the PPLO gravy powder, sodium pyruvate and the 1% phenol red solution are weighed in proportion and dissolved in the deionized water. Before use, healthy horse serum and the specific growth inhibition serum are added. The selective separation medium for the caprine mycoplasmal pneumonia subspecies is used for clinically separating and cultivating the caprine mycoplasmal pneumonia subspecies, increases the growth speed of mycoplasma in a separation process and increases the separation rate of the caprine mycoplasmal pneumonia subspecies, and the separation rate is 80% or higher.

The invention discloses honey-baked peanuts and a processing technology thereof and relates to the technical field of food processing. The technology comprises the following steps: weighing flour, starch, cane sugar, honey, water and vegetable oil for blending, stirring a condiment adhesion agent and peanuts, drying, cooling, and screening, thereby obtaining the product. The honey-baked peanuts and the processing technology thereof disclosed by the invention have the beneficial effects that according to the prepared honey-baked peanuts, any preservative is not added, natural food materials are adopted, and the honey in the formula has the effects of strengthening the middle-Jiao, moistening dryness, relieving pain and detoxifying, is a natural food, consists of monosaccharide type glucose and fructose, can be directly absorbed by the human body, does not need enzymatic decomposition, can be absorbed by the human body without digestion and has good health effects on women and children, particularly the old. Moreover, the peanuts contain rich proteins and amino acids, and have the effects of lowering cholesterol levels and moistening the skin and the peanuts and honey are combined to prepare the honey-baked peanuts disclosed by the invention, and the honey-baked peanuts can achieve the effects of beautifying and promoting health.

The invention discloses lotus flower tea with the effects of relieving palpitation and calming nerves. The lotus flower tea is prepared from, by weight, 120-130 parts of mulberry leaves, 30-35 parts of lotus flowers, 5-10 parts of albizia flowers, 6-7 parts of passion fruits, 10-13 parts of lotus roots, 15-20 parts of stevia rebaudiana, 3-4 parts of dandelions, 4-5 parts of mung bean hulls and a proper amount of plum brine, table salt, rhizopus japonicas and water. The purpose of fixation of mulberry leaves is to inactivate enzymes in fresh mulberry leaves, the raw smell is removed, and kneading is promoted. Dry stevia rebaudiana leaves are treated through an ultrasonic method, and dissolution and extraction of rebaudioside are promoted. The effects of reducing blood glucose, strengthening the body, reducing blood pressure, serving as the sweetening agent and the like are achieved. The taste of the mulberry leaf tea can be improved, and healthcare functions are added. As the plum brine is adopted for processing the fresh flowers, on the one hand, the fresh flowers are cleaned once again, floating dust is washed away, gravel settles, and on the other hand, the color and fragrance of the fresh flowers can be improved. The smell of salted sweet-scented osmanthus flowers is better and more lasting. The scented tea is sweet and mellow in aroma, sweet and soft, and has the effects of clearing internal heat, achieving detoxication, achieving beautifying, relieving palpitation and calming nerves, benefiting the liver and tonifying the spleen, stopping bleeding and benefiting the eyes and the ears, and removing ozostomia.

The invention belongs to the technical field of polydextrose and particularly relates to a production method of the polydextrose. The production process is greatly simplified, materials are not in contact with the outside world and accordingly the secondary pollution to the finished product and the introduction of impurities due to artificial factors are avoided and reduced and the quality of the product is good, and the production cost is low.

The invention relates to a hydrolysis saccharification process of alternanthera philoxeroides. Metal substance pollutants (iron, cobalt and nickel) in alternanthera philoxeroides raw materials are absorbed by adopting magnetic force, so that subsequent mechanical abrasion and contents of metal ions in treating fluid are reduced, and a good fermentation condition is created for detoxification of selected cupriavidus metallidurans; then solid-liquid separation is realized by adopting an extrusion method, hydrolysis on lipids and protein contained in liquid-state materials is carried out by adopting an NaOH solution, and then hydrolysis treatment is further carried out by adopting trypsase, so that the hydrolysis is more completely carried out; meanwhile, extruded solids are dried and crushedand then dropped into a sulfuric acid solution to carry out acidolysis; through electrorheological fluid shear stress effect treatment, cellulase is then added and enzymatic treatment is carried out,so that the effect is good and energy consumption is low. According to the hydrolysis saccharification process of the alternanthera philoxeroides disclosed by the invention, the raw material treatment efficiency is improved to the maximum extent, and finally, saccharified hydrolytic solutions rich in nutrient substances such as glucose, amino acid, small molecule chain lipids and the like are obtained, so that the glucose content is high, and the glucose can be conveniently supplied for further fermentation, and the production efficiency is improved.

The invention discloses a preparation method of an anti-mildew agent for feeds, and belongs to the technical field of feed additives. The preparation method of the anti-mildew agent for feeds comprises the following steps of ball-milling vermiculite, water and betaine so as to obtain a ball-milled material; performing ultrasonic oscillation on the ball-milled material, a hydrochloric acid solutionand cysteine sulfonate, and carrying out freeze-drying so as to obtain a dried material; carrying out fermentation by using distilled water, the dried material, glucose, a yeast extract, edible maggot, fungus powder, disodium hydrogen phosphate and sodium dihydrogen phosphate, and carrying out drying, crushing and sieving so as to obtain sieved granules; putting acetone, ethanol, the sieved granules, diacylglycerol and an additive into a reactor, discharging gas by using trimethylamine, adding an catalyst, carrying out heated stirring, carrying out cooling to room temperature, and carrying out standing; after standing, discharging materials, carrying out filtration, conducting washing by using petroleum ether, diethyl ether, acetone and ethanol, and carrying out drying so as to obtain a dried material; grinding the dried material, a nutrient supplement agent and sodium humate, and collecting the ground material so as obtain the anti-mildew agent for feeds. The anti-mildew agent for feeds solves the problems of the existing anti-mildew agents for feeds, namely liable causing of anorexia and refusal to eat, and slow growth caused by reduced feed intake caused by abdominal fullness of livestock after eating the existing anti-mildew agents for feeds.

The invention discloses nutritional soybean milk containing dark plums. The soybean milk is prepared from raw materials as follows: the dark plums, soybeans, spinach, broad beans, water, sunflower seed oil, yams, sweet osmanthus flowers, Wedelia chinensis, leaves of rough-haired holly, rosemary, glucose and coconut powder. A preparation method of the soybean milk comprises steps as follows: (1) dark plum pulp is prepared; (2) mixed pulp is prepared; (3) broad bean powder is prepared; (4) a nutritional solution is prepared; (5) soybean pulp is prepared; (6) the mixed pulp, the broad bean powder, the nutritional solution, the soybean pulp and the coconut powder are mixed, glucose is added, and the soybean milk is obtained through sterilization after homogenization. Nutritional ingredients such as the dark plums, the spinach and the like are introduced to the soybean milk, meanwhile, health-care and beneficial ingredients such as the yams, the coconut powder and the like are added, the soybean milk has a smooth taste and a unique flavor, contains rich nutritional ingredients such as protein, amino acid, microelements and the like, is easy to digest and absorb and has the relatively good dietary therapy and health-care effects.

The invention provides genetically engineered bacteria synthesizing styrene taking biomass hydrolysate as a raw material and a construction method and application thereof, and relates to the technicalfields of genetic engineering and fermentation engineering. According to the genetically engineered bacteria, L-phenylalanine ammonia-lyase gene, cinnamate decarboxylase gene, 3-deoxy-D-arabinoheptose-7-phosphate synthase gene and bifunctional enzymatic branching acid mutase-prebenzoic acid dehydratase gene are expressed. The construction comprises the steps that the L-phenylalanine ammonia-lyasegene and the cinnamate decarboxylase gene are cloned to be connected to plasmid; the 3-deoxy-D-arabinoheptose-7-phosphate synthase gene and the bifunctional enzyme are cloned to be connected to the plasmid; the plasmid is guided to colibacillus competence to obtain the genetically engineered bacteria. The genetically engineered bacteria can be used for synthesizing the styrene taking the biomasshydrolysate as the raw material and is green and sustainable, the yield of the styrene is greatly higher than the yield of the styrene which is produced by taking the glucose as a carbon source, and the breakthrough which cannot be made all the time is achieved.

The invention belongs to the technical field of animal breeding, and particularly relates to animal fresh semen low-temperature diluent as well as a preparation method and application thereof. The animal fresh semen low-temperature diluent is prepared from the following ingredients: glucose, sodium citrate, penicillin, streptomycin, enrofloxacin, yolk, defatted milk, vitamin A, vitamin D, vitaminE and water. The diluent has the advantages that Australian white sheep semen is stored for 1 to 2 days in a liquid state form under the condition of low temperature being 0 to 5 DEG C; the artificialinsemination requirement can be reached; the fresh semen storage quality is improved, so that the utilization rate of excellent rams is improved; high nanny goat conception rate is obtained; the problem of long-distance transportation after excellent ram semen collection is solved; the manufacturing method of the diluent is simple; the raw material cost is low; the operability is high; the large-scale popularization and application in the production is convenient.

The invention discloses a method for obtaining high-purity glucose by a chromatography process. Starch is used as raw materials; through the hydrolysis processes of liquidation, saccharification and the like, the glucose purity in the saccharification liquid is 95 percent; after the work procedures of impurity removal, decoloring, desalting and the like, evaporation and concentration are performed; the materials enter a simulated moving bed chromatography separation system; a high-purity glucose extraction solution with the purity being 99.5 percent or higher is obtained. The chromatography process is used for producing the glucose; the problems of low yield and long production period of crystalline dextrose are solved; the mother liquid discharging quantity is reduced; the economic valueis improved.