Antigen-specific T lymphocyte freezing medium and preparation method and application thereof
一种淋巴细胞、冻存液的技术,应用在抗原特异性T淋巴细胞冻存液及其制备领域,能够解决制约冻存细胞临床应用、细胞杀伤功能低下、细胞增殖数量不足等问题,达到保持肿瘤杀伤功能、保持复苏成活率、提升细胞活率的效果
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[0066] The second aspect of the present invention provides a method for preparing an antigen-specific T lymphocyte cryopreservation solution, comprising:
[0067] Bomaili Electrolyte Injection, Glucose Sodium Chloride Injection, Dextran Glucose Injection and Human Serum Albumin Solution are 30%-40%, 30%-40%, 5%-15% and 15%- Mix at a ratio of 25% to prepare freezing solution A;
[0068] Bomaili Electrolyte Injection, Glucose Sodium Chloride Injection, Dextran Glucose Injection, Human Serum Albumin Solution and Dimethyl Sulfoxide are 20%-30%, 20%-30%, 5%- 15%, 15%-25% and 10%-20% were mixed to prepare freezing solution B;
[0069] Freezing solution A and freezing solution B are stored separately. When used, the freezing solution A and freezing solution B are mixed according to the volume ratio of 1:0.5-2 to obtain the freezing solution.
[0070] In the present invention, cryopreservation solution A and cryopreservation solution B are mixed according to a volume ratio of 1:1. ...
Embodiment 1
[0094] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, where the cryopreservation solution A includes the following volume fraction components:
[0095] 35% Bomaili Electrolyte Injection, 35% Glucose Sodium Chloride Injection, 10% Dextran Glucose Injection and 20% Human Serum Albumin Solution;
[0096] Freezing solution B includes the following components by volume fraction:
[0097] 27.5% Bomaili Electrolyte Injection, 27.5% Glucose Sodium Chloride Injection, 10% Dextran Glucose Injection, 20% Human Serum Albumin Solution and 15% Dimethyl Sulfoxide;
[0098] Below are manufacturer's brand and article number of each raw material that adopts in the embodiment of the present invention:
[0099] Table 1 Raw material list
[0100]
[0101] The glucose sodium chloride injection contains 5% dextrose and 0.45% sodium chloride, and the dextran glucose injection contains 10% low molecular weight...
Embodiment 2
[0104] A cryopreservation solution for antigen-specific T lymphocytes, including cryopreservation solution A and cryopreservation solution B, where the cryopreservation solution A includes the following volume fraction components:
[0105] 30% Bomaili Electrolyte Injection, 40% Glucose Sodium Chloride Injection, 5% Dextran Glucose Injection and 25% Human Serum Albumin Solution;
[0106] Freezing solution B includes the following components by volume fraction:
[0107] 30% Bomaili Electrolyte Injection, 20% Glucose Sodium Chloride Injection, 5% Dextran Glucose Injection, 25% Human Serum Albumin Solution and 20% Dimethyl Sulfoxide;
[0108] The glucose sodium chloride injection contains 5% dextrose and 0.45% sodium chloride, and the dextran glucose injection contains 10% low molecular weight dextran and 5% dextrose;
[0109] Cryopreservation solution A and cryopreservation solution B are stored separately. When in use, cryopreservation solution A and cryopreservation solution B...
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