31 results about "In vitro" patented technology

The present invention relates to an aqueous alcohol buffer solution for substantially ambient, in vitro preservation of mammalian cells for a selected duration.

Disclosed are libraries of DNA sequences encoding zinc finger binding motifs for display on a particle, together with methods of designing zinc finger binding polypeptides for binding to a particular target sequence and, inter alia, use of designed zinc finger polypeptides for various in vitro or in vivo applications.

The invention provides a polyethylene glycol-polyamide-amine-polyamino acid linear-dendritic block polymer with a structure shown as a formula I, II, III or IV. The polyethylene glycol in the block polymer can be used for improving the biocompatibility of the block polymer and prolonging the circulation time of the block polymer in the blood, and a polyamino acid block can be used for ensuring the favorable degradation performance of a product and can be discharged in vitro after being degraded in vivo. The block polymer provided by the invention is simple in preparation method and safe and nontoxic in product. The block polymer is used as a carrier and assembled in water to form nanoparticles, and a model drug adriamycin is carried, so that drug-carried nanoparticles are obtained, and the drug-carried nanoparticles have a certain controlled-release function and can be used for effectively inhibiting the proliferation of cancer cells in cell experiments. Therefore, the block polymer has the potential application as a nano-drug carrier.

The invention discloses a culture method and a culture container for an in-vitro sterile host plant-mycorrhizal edible fungus intergrowth seedling. The culture method comprises the steps of (1) preparing an in-vitro sterile host plant; (2) sterilely and accurately inoculating; and (3) culturing the host plant-mycorrhizal edible fungus intergrowth seedling. The culture container comprises a transparent container, a container cover and a triangular fixing frame. A filtration ventilating window is arranged on the container cover; the triangular fixing frame is arranged on the bottom part of the container; a circular ring with a notch is arranged on the upper part of the triangular fixing frame; and a plurality of outwards hooks for fixing an elastic rubber ring are arranged on the upper part of the circular ring. According to the culture method and the culture container for the in-vitro sterile host plant-mycorrhizal edible fungus intergrowth seedling provided by the invention, a host plant system can be cultured under the controlled environment conditions of temperature, humidity and illumination, so that the intergrowth seedling can be bred quickly and largely; and compared with the prior art, the breeding speed is improved by 3 to 12 times, the inoculation success rate is improved by 1.6 to 2.5 times, and the production rate of the inoculated host plant-mycorrhizal edible fungus intergrowth seedling is improved by 30 percent to 50 percent.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The present invention relates to in vitro methods for determining efficacy of topical compositions on various skin color types. These methods involve providing an artificial skin apparatus configured to approximate the color and diffuse reflectance characteristics of a predetermined human skin color type. The artificial skin apparatus includes an artificial skin substrate combined with a color background, with the color background correlating to the human skin color type. A topical composition of interest is applied to the artificial skin substrate of the artificial skin apparatus and then pre-irradiated. The pre-irradiated topical composition is then analyzed using relevant experimental techniques or assays for at least one efficacy parameter, including, for example, anti-ageing, photoprotective, sun protective, UVA/UVB protective, UVAI protective, photo stabilizing, and photosensitizing activities and action mechanisms of the topical composition on various skin color types. Also provided is an artificial skin apparatus and kit for use with the in vitro method.

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

The present invention relates to a layered cell sorted tissue that is formed in vitro. The tissue is generated by the spontaneous sorting of cells from a homogenous cell mixture into discrete layers by cell type. Connective tissue components, such as fibronectin, may be used to manipulate orientation of the layers during the cell sorting process. The layered cell sorted tissue may be used as a skin graft for bums, wounds, and ulcers. The tissue may also be used in assays to determine effects of chemicals or drugs on human tissue in vitro as well as provide an in vitro assay for tumor cell metastasis.

The invention provides a wheat scab resistance identification method. Wheat scab resistance is identified in a short time at high throughput in case of controllable environmental conditions by utilizing in-vitro ears of wheat. The method disclosed by the invention has the advantages that a novel scab resistance evaluation standard is discovered, and the wheat scab resistance is evaluated accordingto whether black dense necrotic spots are produced at inoculation positions. By utilizing the standard, limitations of external environmental conditions can be avoided, and the wheat scab resistancecan be simply and rapidly identified within 4-5 days. The method is intuitive and reliable in phenotype identification and excellent in repeatability, is capable of completing resistance identification of batches of materials in a short time, and has significances on genetics and breeding researches of wheat scab resistance all over the world.

The invention discloses lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance and belongs to the technical field of food. The lactobacillus plantarum CGMCC12436disclosed by the invention is amplification cultured under assistance of skimmed milk, trehalose and saccharose; after being frozen and dried, the lactobacillus plantarum is prepared into a biologicalpreparation; a viable bacterial content of the lactobacillus plantarum CGMCC12436 in the preparation is larger than 109CFU/g. The lactobacillus plantarum CGMCC12436 disclosed by the invention has theadvantages of lower growth generation time, high survival rate in in-vitro simulated gastric fluid and intestinal juice, good adhesion on human HT-29 cells and relatively lower drug resistance in drug allergy testing. The biological preparation prepared from the lactobacillus plantarum has the advantages that ampicillin induced intestinal flora disturbance in a mouse body can be obviously recovered, intestinal functions can be improved and has good market application prospect.

The invention specifically relates to a method for estimating in-vitro detoxification effect of a mycotoxin detoxification agent by using liquid chromatography, belonging to the technical field of biological detection. The method comprises the following steps: (1) early stage preparation: I, drafting of a standard curve of mycotoxin; II, preparation of a sample of the detoxification agent; and III, acquisition of a reaction medium; (2) detoxification: I, evaluation of in-vitro adsorption detoxification; and II, evaluation of simulated in-vivo adsorption detoxification; and (3) liquid chromatographic detection. The method for estimating in-vitro detoxification effect of the mycotoxin detoxification agent by using liquid chromatography can simulate gastrointestinal environments, adopts supernatant of chyme in the alimentary canal of an animal as a reaction medium, completely reproduce the gastrointestinal environment in the animal and more authentically evaluate the adsorption effect of an adsorbent.

The invention relates to the field of biomedicines, in particular to a method for inducing differentiation of stem cells into islet-like cells in vitro and use of islet-like cells. The invention aims to provide a method for obtaining a large amount of islet-like cells in vitro and the use of the islet-like cells differentiated under induction as seed cells in transplantation of islet cells and preparation of microencapsulated stem cell preparation and as an in-vitro pharmacokinetic model in cell medicine screening. For realizing the aim, the invention adopts a technical scheme that: 1) constructing an NRSF interfering carrier; 2) separating, culturing and amplifying stem cells; 3) inducing stem cells to differentiate into islet-like cells by two stages; and 4) identifying islet-like cells. The construction of the method has a very bright application prospect in regenerative medicine taking gene engineering, cell engineering, tissue engineering and the like as a basis and is expected to create huge social and economic benefit.

Disclosed are compositions and methods of making stable electrically active adult neurons from adult neural tissue. The disclosed compositions can be used with microelectrode arrays in vitro to represent in vivo neural function for drug discovery and for studying neuronal degenerative diseases, neuronal development, and neuronal regeneration.

The invention provides a phosphatidyl nanometer prodrug released by enzymatic response and a preparation method and application thereof. The phosphatidyl nanometer prodrug is formed by a hydrophilia antitumor drug containing primary alcohol and a phospholipid compound through bonding of a phosphorus oxygen bond under the catalytic action of phospholipase D. The invention further provides application of the phosphatidyl nanometer prodrug released by enzymatic response for releasing the antitumor drug to tumor tissue in a targeting mode. The hydrolysis/transesterification two-way reaction characteristic of the phospholipase D highly-expressed in tumor tissue is utilized creatively, and the amphipathicity phosphatidyl nanometer prodrug is obtained through biological catalytic synthesis of the phospholipase D in vitro; when the prodrug is accumulated to tumors in a targeting mode for responding to the phospholipase D to trigger hydrolysis, the antitumor drug is specifically released, and meanwhile the purposes of safety, stability and effective release of the targeting medicine are achieved.

The invention relates to a beta-carboline compound and a synthesizing method and application thereof, and belongs to the technical field of medicines. The structure of the compound is shown in a formula I. Being proved by preliminary experiment, the beta-carboline compound has the advantages that the activity of resisting renal fibrosis in vitro is obvious, and the good potential medical value is realized; the beta-carboline compound can be applied to prepare various types of medicines for resisting renal fibrosis and chronic renal diseases. The formula I is shown in the description.

The present invention relates to methods for obtaining transgenic embryos, animals, and plants. Also encompassed in the present invention are transposase-mediated trangenesis methods including transposase-mediated intracytoplasmic sperm injection (TN:ICSI), transposase-mediated intracytoplasmic round spermatid injection (TN:ROSI), and transposase-mediated in vitro fertilization (TN:IVF).

The invention discloses an in-vitro heart constant pressure/constant flow perfusion experiment device for cardiological mammals. The device comprises a fixing and supporting device, a perfusion device and a monitoring device. The fixing and supporting device comprises a bottom platform. A fixed supporting plate and a movable supporting plate are arranged at the rear side of the bottom platform. A hydraulic lifter is arranged below the movable supporting plate. The perfusion device comprises a baffling perfusion column, and the baffling perfusion column is fixedly arranged on the front face of the movable supporting plate. The bottom of the baffling perfusion column is connected with the heart, and a conical incubator and a blood filter are sequentially arranged below the heat. The right side of the blood filter is connected with an oxygenator, and the oxygenator is connected with a gas storage bottle, a liquid storage bottle and a peristaltic pump through a gas conveying pipe, a liquid replenishing pipe and a liquid conveying pipe, respectively. The tail end of the liquid conveying pipe is connected with a liquid inlet of the baffling perfusion column. The baffling perfusion column and the conical incubator are connected with a temperature controller by means of a manifold. The monitoring device collects and records the test data through an electrode, a balloon, a heart sound transducer and a pressure transducer.

The invention belongs to the technical field of biological preparation and in particular relates to an RXRalpha (Homo Sapiens Retinoid X Receptor alpha) gene knockout cell system with stable and low expression of an RXRalpha protein and a preparation method of the RXRalpha gene knockout cell system. The cell system is an RXRalpha gene knockout cell system; the knockout base number is an integral multiple of a number except 3; RXRalpha protein expression in a cell is remarkably reduced, the genotype is stable and can be passed to next generations, the stability of the RXRalpha low-expression cell system is greatly improved, and stable generation passage and continuous culture of the cell system can be facilitated. The invention provides the preparation method of the cell system. The preparation method comprises the following steps: according to RXRalpha gene sequence information, designing a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) knockout gRNA (Guide Ribonucleic Acid) sequence, establishing a gRNA expression vector, and carrying out in-vitro cell level detection on gRNA shearing activity; by using a nuclear transferring method, carrying out cotransfectionon an immortalized cell of human neuroblastoma, that is, an SK-N-SH cell, by using cas9 and the gRNA expression vector, carrying out G418 medicine screening so as to obtain stable cell cloning, carrying out a PCR (Polymerase Chain Reaction) and gene sequencing to identify that the cell is cell cloning of which the RXRalpha gene is knocked out for an integral multiple of a number except 3, therebyobtaining the RXRalpha gene knockout cell system with stable and low expression of the RXRalpha protein.

The invention discloses a panax notoginseng reverse osmosis protein gene PnOLP1. The gene PnOLP1 has a nucleotide sequence shown as SEQ ID NO:1, and encodes protein having an amino acid sequence shownas SEQ ID NO:2. It is verified by functional genomics related technical research that the gene PnOLP1 has the function of improving the antifungal property of plants; after the antifungal gene PnOLP1is constructed onto a plant expression vector and transferred into nicotiana tabacum for overexpression, the transgenic nicotiana tabacum plant has quite high in-vitro antifungal activity as a result, and the experimental result shows that the transgenic nicotiana tabacum overexpressing PnOLP1 has a significant inhibiting effect on the growth of four fungi including colletotrichum gloeosporioides, fusarium solani, fusarium oxysporum and nigrospora oryzae.

The invention discloses an ailanthus altissima efficient in-vitro culture plant regeneration method. The method comprises the procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture, and comprises the following specific steps: collecting a semi-lignified robustly-growing annual ailanthus altissima shoot to serve as an explant; trimming the explant, sterilizing and disinfecting the trimmed explant, and inoculating the sterilized and disinfected explant into an initial bud induction culture medium to obtain an initial bud; inoculating the initial bud into a multiplication culture medium, and performing multiplication culture for 3 to 4 periods to form cluster buds; inoculating the cluster buds into a bud strengthening culture medium for culturing; lastly, inserting single buds which are more than or equal to 2cm in heights into the rooting culture medium to induce rooting. By adopting the method, the ailanthus altissima seedling period is shortened, the seedling material is saved, the problems of need of a large quantity of seedling materials, long period and the like in the conventional seedling method are solved, the production cost is lowered greatly, the production efficiency is increased, and good economical benefit, social benefit and ecological benefit are achieved.

The invention discloses a hydrophilic hollow silicon sphere as well as a preparation method and application thereof. The hydrophilic hollow silicon sphere is prepared by modifying the surface of a silicon-oxygen structured double-bond silicon sphere, which is formed by performing catalytic reaction on vinyltriethoxysilane and is used as a core, with glutathione, and etching a sphere center with hydrofluoric acid. Drug encapsulation and in-vitro simulated releasing experimental results show that compared with a hollow silicon sphere of a contrast group, the hollow silicon sphere prepared with the preparation method disclosed by the invention has a significant difference in drug loading and in-vitro releasing of camptothecin, so that the hydrophilic hollow silicon sphere prepared with the preparation method is relatively high in loading capacity and relatively good in controlled release effect. The hydrophilic hollow silicon sphere is simple in design; no reaction conditions such as high temperature and high pressure are needed; the particle size distribution of the silicon sphere is uniform; feasibility is provided for industrial production of the hydrophilic hollow silicon sphere; a new way is provided for researches on drug slow release carriers; the hydrophilic hollow silicon sphere has good social and economical benefits.

The invention discloses medical equipment for platelet-rich plasma gel (PRG) in-vitro negative pressure cutting treatment, which comprises a box body, the box body is connected with a box cover through screws, the box body is provided with a sealing groove, the box cover is adhered with a silica gel ring, the sealing groove is internally and slidably connected with the silica gel ring, the box cover is provided with a lifting stirring mechanism, and the lifting stirring mechanism is connected with the box cover through screws. A high-speed cutting mechanism is arranged on the box body, an ultraviolet lamp is fixedly installed on the box cover, the side wall of the box body is fixedly sleeved with a PRP guide port, the side wall of the box body is fixedly sleeved with an activator guide port, a suction pump is fixedly installed on the side wall of the box body, and four supporting feet are welded to the box body and evenly distributed on the box body. The invention relates to medical equipment for PRP (Platelet Rich Plasma Gel) in-vitro negative pressure cutting treatment. The medical equipment has the characteristics that a relatively high sterile level can be kept when PRP is cut in vitro, and the cut PRP can be subjected to proper form cutting treatment aiming at tissues of different injured parts.

Identification and use of compounds which inhibit the expression or activity of micro-RNAs for preventing and/or attenuating ageing. An in vitro method for screening for candidate compounds for preventing and/or attenuating ageing of the skin including (a) bringing at least one test compound in contact with a sample of fibroblasts, (b) measuring the expression or the activity of at least one microRNA chosen from miR-134 and miR-152 in said fibroblasts, and (c) selecting the compounds for which an inhibition of at least 20%, preferably at least 30%, preferably at least 40% of the expression or an inhibition of at least 20%, preferably at least 30%, preferably at least 40% of the activity of at least one microRNA is measured in the fibroblasts treated in (a) compared with the untreated fibroblasts.

Provided are a kit and method of in vitro auxiliary diagnosis of pancreatic cancer. Particularly, provided is an application of human serum miR-25 as a test target in preparing a pancreatic cancer diagnostic reagent. Also provided is a kit for in vitro auxiliary diagnosis of pancreatic cancer, the kit comprising primers and probes for real-time fluorescent quantitative PCR detection of human serum miR-25.

The invention discloses application of LF/DHA-SPI/DHA micro-aggregate in foods for infants and crowds with gastrointestinal tract fragility. According to the application disclosed by the invention, the micro-aggregate having favorable DHA in vitro simulation digestion characteristics is constructed around a special-shaped aggregation effect, an effective construction way of the DHA micro-aggregate is explored, the DHA in vitro simulation digestion is improved, and a bran-new solution is provided for DHA steady state in application, so that a guiding effect is provided for the application of the LF/DHA-SPI/DHA micro-aggregate.

The invention relates to the technical field of epilepsy model construction, in particular to a new method for inducing epilepsy models through drugs, namely a new application method of a pharmacological tool drug. The epilepsy models are induced in vivo and in vitro. KN93 serves as the pharmacological tool drug to be applied to building and inducing the epilepsy animal model and the epilepsy cellmodel. The KN93 in the method can simultaneously induce the epilepsy animal model and the epilepsy cell model, and an epilepsy model caused by drugs in the prior art is one of an animal model (in vivo) and a cell model (in vitro), and therefore the KN93 can induce the epilepsy models in vivo and in vitro; then an epilepsy disease mechanism is deeply discussed and related anti-epileptic drugs arescreened.