Ailanthus altissima efficient in-vitro culture plant regeneration method

A technique for in vitro culture and Ailanthus sinensis tree is applied in the field of plant reproduction, which can solve the problems of slow growth and low bud proliferation coefficient, and achieve the effects of large yield of sub-generation buds, simple operation process and excellent quality.

Inactive Publication Date: 2017-05-17
唐春艳 +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of stronger plants from seeds collected by cutting them down off trees without damaging their roots during growth periods. By inserting new shoots called 'Ailsanches' instead of older ones, these plantlets have better chance of developing roots compared to previous methods like graftings. Additionally, this technique helps save money while producing consistently higher yields of young crops. Overall, this innovative approach simplifies the way we grow crop varieties and increases productivity.

Problems solved by technology

This patented technical problem addressed by this patents relates to creation of forest trees that have superior properties such as fire retardancy, moisture tolerances, durable welds, ease at different levels of temperature, adaptation to varying types of terrain, resistance against harmful chemical substances like pesticides, prevention from damage caused by rainwater, saltiness, polluted environments, and extreme environmental factors including cold winters and dry summers.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Ailanthus ailanthus in the same year as explants;

[0026] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0027] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM

Embodiment 2

[0033] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Ailanthus ailanthus in the same year as explants;

[0034] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 mod

Embodiment 3

[0041] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Ailanthus ailanthus in the same year as explants;

[0042] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 imp

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ailanthus altissima efficient in-vitro culture plant regeneration method. The method comprises the procedures of explant selection, explant treatment, initial bud induction culture, multiplication culture, strong bud culture and rooting culture, and comprises the following specific steps: collecting a semi-lignified robustly-growing annual ailanthus altissima shoot to serve as an explant; trimming the explant, sterilizing and disinfecting the trimmed explant, and inoculating the sterilized and disinfected explant into an initial bud induction culture medium to obtain an initial bud; inoculating the initial bud into a multiplication culture medium, and performing multiplication culture for 3 to 4 periods to form cluster buds; inoculating the cluster buds into a bud strengthening culture medium for culturing; lastly, inserting single buds which are more than or equal to 2cm in heights into the rooting culture medium to induce rooting. By adopting the method, the ailanthus altissima seedling period is shortened, the seedling material is saved, the problems of need of a large quantity of seedling materials, long period and the like in the conventional seedling method are solved, the production cost is lowered greatly, the production efficiency is increased, and good economical benefit, social benefit and ecological benefit are achieved.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner 唐春艳
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap