Culture method and culture container for in-vitro sterile host plant-mycorrhizal edible fungus intergrowth seedling

[0019] A culture container for isolated aseptic host plant-mycorrhizal edible fungus symbiotic seedlings, comprising a transparent container 1, a container cover 2 and a triangular fixing frame 3. Described triangular fixed mount 3 is arranged on the bottom of container 1, and the top of triangular fixed mount 3 is provided with a circular ring 4 with breach, and the circular ring 4 of breach is provided with some outwards, fixed elastic rubber ring 6 Small hook 5. The center of the container cover 2 is provided with a filter ventilation window 7 .

[0021] A method for cultivating an isolated aseptic symbiotic seedling of oak and truffle, comprising the steps of:

[0022] (1) Preparation of host material Cut the branches of oak (Quercus liaotungensis or Quercus cork) into 1-inch long sections, each section of which contains an axillary bud, remove the petiole of the leaves, wash them with clean water, and then use 70% alcohol for surface sterilization. Sterilize for 30 seconds, then wash 3 times with sterilized water, then sterilize with 0.1% sodium hypochlorite for 3-5 minutes, pour off the sodium hypochlorite, rinse 3 times with sterilized water, and set aside.

[0023] (2) Culture medium preparation Select the WPM medium suitable for the cultivation of oak shoot tips as the basic medium, add 0.2-0.5% 6-BA, 3% sucrose and 15g / L agar powder, and adjust the pH value with NaOH or HCl 6.5-7.0. The culture medium is divided into 150-180mm×180mm test tubes, each test tube is divided into 10ml of medium, and sterilized in an a

[0029] A method for cultivating the isolated aseptic symbiotic seedlings of poplar and boletus deliciosa, comprising the steps of:

[0030] (1) Preparation of host plant material Aspen is one of the host plants of the delicious boletus. Select the branches of the aspen with leaf buds, and arrange the branches according to the method of step (1) in Example 1, and wash the branches. Clean, sterilize the surface, and reserve.

[0031] (2) Medium preparation The medium suitable for the host plant is the key. The suitable medium for poplar is MS+0.3-0.5%6-BA+3% sucrose+15g / L agar powder, with a pH value of about 6. It is prepared according to the preparation method in step (2) of Example 1, and it is sterilized for later use.

[0032] (3) In vitro culture of the host plant Take the small sections of poplar branches of the above (1), under the microscope in a sterile ultra-clean workbench, pick out the stem tip of about 0.5 mm, put it into (2) medium, and put it in the sterile ultra