6 results about "Aptamer" patented technology

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

The invention discloses a magnetic MOF@aptamer and a method for high-sensitivity fluorescence detection of food-borne pathogenic bacteria by the same, which mainly comprises the following steps: preparing Fe3O4@ZIF-8 by solvothermal and layer-by-layer self-assembly method, modifying the Fe3O4@ZIF-8 by using the aptamer of target bacteria to prepare Fe3O4@ZIF-8@aptamer, capturing and separating the target bacteria, furthermore, using the FITC fluorescence labeled antibody for identifying the target bacteria, so that the fluorescence resonance energy transfer between the FITC fluorescence molecule and the surface of the Fe3O4@ZIF-8 metal group is realized, and the fluorescence enhancement of the FITC is realized. The research method disclosed by the invention has the advantages of high detection sensitivity, strong specificity, short detection time and the like, and is suitable for rapid detection of food-borne pathogenic bacteria in food samples.

The invention discloses a cross-correlation detection system based on up-conversion and fluorescence resonance energy transfer nanoparticles. The system comprises a fluorescence cross-correlation spectroscopy detection module, an aptamer, up-conversion particles and fluorescence resonance energy transfer nanoparticles. The two ends of the aptamer are modified with the up-conversion particles and fluorescence resonance energy transfer nanoparticles. The fluorescence cross-correlation spectroscopy detection module excites the up-conversion particles and fluorescence resonance energy transfer nanoparticles to emit radiation spectrum separation light and detects fluorescence signals of the two kinds of light respectively. A computer carries out processing to obtain autocorrelation and cross-correlation spectra of the two kinds of light; and the autocorrelation and cross-correlation spectra are analyzed precisely to obtain parameters like the aptamer concentration. According to the invention, the selected up-conversion particles and fluorescence resonance energy transfer nanoparticles have the large anti-Stokes shift, so that the signal light, excited light, and environment noises are separated conveniently and the interference of background noises is reduced. Meanwhile, because of the up-conversion particles and fluorescence resonance energy transfer nanoparticles with large spectrum intervals, the interference between channels is reduced and the detection accuracy and result accuracy are improved.