Detection method of tetracycline

[0034] Example 1: Next, MOS 2 The nanoflakes are described in a detailed description of the above synthesis.

[0035] Preparation of a piece of MOS in 6 layers 2 Nanoflane, dissolved in Tris-HCl buffer solution;

[0036] A surface modified CDSE quantum dot is modified;

[0037] Preparation of a tetracycline adapter single-strand DNA: -HOOC-CGT ACG ATG CAG-COOH;

[0038] -HOOC-CGT ACG ATG CAG-COOH is dissolved in pH = 6.0, Tris-HCl buffer solution, and stirred for 2 hours, resulting in quantum dots - adapter solution;

[0039] Wrap the laminate MOS 2 The solution is added to the quantum dot - adapter solution, quench the fluorescence of the quantum dots - adapter;

[0040] Further, a tetracycline solution was added to the above solution, and the fluorescence intensity of the system was measured after 10 minutes of incubation for 30 minutes, and the system was fluorescence.

[0041] Example 2: The following is VTE 2 The nanoflakes are described in a detailed description of the above synthesis.

[0042] Preparation of a piece of VTE for 10 layers 2 Nanoflane, dissolved in phosphate buffered solution;

[0043] A surface modified INASP quantum dot is modified;

[0044] Preparation of a tetracycline adapter single-stranded RNA: -HOOC-CGT ACG CUA UAG CGU CAG-COOH;

[0045] Plus -HOOC-CGT ACG ATG CAG-COOH and the surface modified an amino-based INASP quantum dot is dissolved in pH = 7.0, phosphate buffered solution, stirred for 20 hours;

[0046] Wrap the sheet-like VTE 2 The solution is added to the quantum dot - adapter solution, quench the fluorescence of the quantum dots - adapter;

[0047] Then, a tetracycline solution was added to the above solution, and the fluorescence intensity of the system was measured after incubation at room temperature, and the detection was fluorescence.

[0048] In summary, the method of detecting a tetracycline provided by t...