16 results about "Buffer solution" patented technology

The present invention relates to an aqueous alcohol buffer solution for substantially ambient, in vitro preservation of mammalian cells for a selected duration.

The invention provides a CDs-Pt nanomaterial with catalase catalytic properties. The CDs-Pt nanomaterial shows good enzyme-like catalytic activity in an acetate buffer solution using TMB as a substrate. The CDs-Pt nanomaterial provided by the invention belongs to the field of catalytic material and mimic enzyme research, and the obtained CDs-Pt nanomaterial has the characteristics that carbon quantum dots are modified by precious metal platinum, the synthetic steps are simple, the material scale is small, and the CDs-Pt nanomaterial has good dispersion in a reaction system, and has broad prospect in actual application such as hydrogen peroxide detection and glucose detection.

The invention discloses a method for quickly detecting aflatoxin B1 by PbS quantum dot. The method is characterized by comprising the steps of: adding Pb(NO3)2 solution into mercaptoacetic acid and Na2S solution to prepare PbS quantum dot; preparing 10mug/mL QD-Ab solution by coupling of PbS quantum dot and aflatoxin B1 monoclonal antibody; adding aflatoxin B1-bovine serum albumin conjugate in an elisa plate to coat the elisa plate, and simultaneously adding AFB1 solution and QD-Ab solution, after incubation digestion, adding Hg2Cl solution, getting constant volume by adding acetic acid buffer solution, then preparing the to-be-detected solution; deoxidizing the to-be-detected solution, adding the deoxidized to-be-detected solution into a pretreated glassy carbon electrode for detection, and calculating the content of the aflatoxin B1 according to a standard curve relation between the dissolving peak size of Pb<2+> and the concentration of aflatoxin B1 solution. The method has the advantages of high sensitivity, strong selectivity, excellent stability, high accuracy and quick detection speed.

The invention relates to a novel orthobunyavirus fluorescence quantitative RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) detection kit and a novel orthobunyavirus detection method, and aims to provide the kit which is convenient to use and accurate in detection and the detection method which is high in accuracy and high in detection efficiency. The technical scheme is that: the novel orthobunyavirus fluorescence quantitative RT-PCR detection kit comprises a specific primer, a fluorescent probe, a PCR buffer solution, dideoxyribonucleotide triphosphate and a mixed preparation, wherein an upstream primer has a sequence of FP:5'-TGGCCCTGGCTCTATAAACAT-3'; a downstream primer has a sequence of RP:5'-AATGGCAGCCCAAATGAATC-3'; the specific probe has a sequence of P:5'-FAM-TCCAATGAYGCCAAGAAGTGGAA-BHQ1-3'; FAM is a fluorescent reporter group; and BHQ1 is a fluorescent quenching group.

The invention relates to an electrochemical method for detecting banned additive urotropin in food, which comprises the following steps of: respectively and simultaneously inserting a working electrode carbon paste electrode, a reference electrode saturated calomel electrode and a counter electrode platinum sheet into 0.1mol.L-1PBS (Phosphate Buffer Solution) buffer solution to carry out differential pulse voltammetry scanning to obtain the oxidation peak current value of the urotropin, wherein the pH of the buffer solution is 6, the buffer solution contains a series of urotropin standard solution in different concentrations, and the oxidation peak current value and the concentration of the urotropin have a good linear relationship; with the same method, the oxidation peak current value of the sample solution is obtained; and according to a standard curve and a corresponding linear regression equation, the density of the urotropin in the sample is obtained. The method is simple in operation, is convenient and quick and has a low detection cost, and the urotropin can be directly detected.

The invention discloses a kit for detecting the content of a KAPPA light chain.The kit comprises a reagent R1, a reagent R2 and a KAPPA light chain calibration material, wherein the reagent R1 comprises a first buffer solution, a first electrolyte, a surface active agent, a first stabilizer, a macromolecule accelerant and a first preservative, the reagent R2 comprises a second buffer solution, an anti-human KAPPA light chain antibody, a second electrolyte, a second stabilizer and a second preservative, and the C1 inhibitor calibration material comprises a third buffer solution, a third stabilizer, a third preservative, an antioxidant and a KAPPA light chain antigen.The invention further discloses the application of the kit for detecting the content of the KAPPA light chain and a method for detecting the content of the KAPPA light chain using the kit.By the adoption of the kit and the detection method, the content of the KAPPA light chain can be detected easily and quickly.

The invention relates to a kit for quantitatively detecting angiotensin converting enzyme. The kit contains the following components: 1.0-2.0 mmol/L furan acryloyl phenylalanyl glycyl glycine (FAPGG),80-100 mM buffer solution (pH 6.5-8.5), 0.5-1% stabilizer (W/V), 0.01-0.05% preservative (W/V), wherein a solvent is deionized water. The kit can be used for rapid quantitative detection of the concentration of angiotensin converting enzyme in blood, and has the characteristics of simple operation, high accuracy, wide applicability and long effective period.

The invention relates to a preparation method of dendrobium officinale fermentation liquid. The method comprises the following steps of (1) preparing food enzymatic hydrolysate; (2) preparing a dendrobium officinale extraction solution; (3) preparing a dendrobium officinale nutrition solution; (4) performing fermentation; (5) performing emulsification. The dendrobium officinale, moringa oleifera powder and various medical and edible materials are matched in a proper proportion, and are sufficiently mixed; then, lactobacilli are added for fermentation; single strains, i.e., the lactobacilli are used in the fermentation process; a purpose-made culture solution is used as the breeding ground; the fermentation efficiency is more sufficiently realized; the combination of sodium chloride and acetic acid is used as a buffer solution, so that the pH value of the solution is enabled to be maintained in the pre-control range in the fermentation process.

This invention provides a sort of suspension that can cure the liver transplant rejection, comprising bone marrow mesenchymal stem cells receptor, buffer solutions, bone marrow hematopoietic stem cells receptor in addition. The preparation of the said suspension is separate bone marrow mesenchymal stem cells and bone marrow hematopoietic stem cells from bone marrow receptor first, then adjust the cell concentration of bone marrow mesenchymal stem cells receptor to 0.25 to 1*106 per milliliter or adjust both cell concentration to 0.25 to 1*106 per milliliter, then mix them in proportion to 1 to 1. As the stem cells in the said suspension that can cure the liver transplant rejection are from the receptor itself, it can not only cure the liver transplant rejection, but also very safe, and almost no adverse reaction, no more exogenous pollution and disease transmission problems.

The invention discloses application of sucrose phosphorylase in preparation of 2-O-alpha-D-glucosyl-L-ascorbic acid. According to the application, a reaction system is formed by taking fermentation liquor, obtained through fermenting sucrose phosphorylase gene containing engineering bacteria, or broken liquor, obtained through resuspending wet thalli obtained after centrifuging the fermentation liquor with a buffer solution, as a catalyst, taking L-ascorbic acid as a substrate, taking sucrose as a cosubstrate and taking a buffer solution with a pH of 4.8 to 6.0 as a reaction medium, and a reaction is carried out at the temperature of 30 DEG C to 55 DEG C, thereby obtaining AA-2G. According to the application, the AA-2G is efficiently produced by employing the sucrose phosphorylase, the yield is 3.47g/L/h and is increased by 34.5% compared with that employing the existing reported B. longum derived sucrose phosphorylase, and the maximum concentration of the AA-2G can reach 178g/L; and recombinant bacillus subtilis can efficiently secrete and express the sucrose phosphorylase, and the fermentation liquor is directly applied to preparation of food-grade AA-2G.

The invention discloses a method for colorimetric detection of hydrogen peroxide by utilizing metal organic complexes. The method comprises the following steps of: (1) preparing inorganic salt solution of valence-variable metals into solution I; (2) preparing 2-methylimidazole solution or 4,4-dipyridyl into solution II; (3) mixing the solution I with the solution II, so as to prepare metal organic complex solution; and (4) mixing dimethyl sulfoxide solution of peroxidase color source substrates and the metal organic complex solution with a to-be-tested hydrogen peroxide sample, diluting the solution by utilizing buffer solution to obtain solution A, standing, testing the absorbance value of the solution A in a visible light region, and detecting the concentration of the hydrogen peroxide by utilizing the colorimetric detection. According to the method disclosed by the invention, the detection time can be greatly shortened, the operation is simple, the cost is low, and the disadvantages that the cost is high when metal nanoparticles are utilized and the reaction time is long when mimic enzymes such as metallic oxide are utilized are avoided.

The invention relates to the technical field of cosmetics, and provides a toning lotion which comprises the following components in percentage by weight: 79-99.8% of an acidic buffer solution, 0.001-20% of an alcohol substance and 0.001-1% of agar, wherein the acidic buffer solution comprises hydroxy acid and water, and the acidic buffer solution further comprises at least one of hydroxy acid salt and a pH regulator. According to the toning lotion provided by the invention, hydroxy acid contained in the acidic buffer solution has moisture retention, oxidation resistance and film-forming property, can form an acidified film on the skin surface and maintain the acidity of the skin surface, and meanwhile, the hydroxy acid can form the acidic buffer solution with at least one of the hydroxy acid salt and the pH regulator, so that the toning lotion has an acid-base buffering capability, and the toning lotion has the advantages that the toning lotion has a good moisturizing effect; the pH value change of the skin surface in the skin metabolism process or under the acid-base stress can be buffered, so that the pH value deviation of the skin surface is corrected, the acid environment of the skin is recovered, and the skin barrier function is reset.

The invention discloses a novel method for purifying an arsenic chelating type immune complex. The method comprises steps as follows: S1, a chelating agent solution is prepared firstly through dissolution of a chelating agent in a solvent; S2, a proper amount of carrier protein is taken and added to a borate buffer solution, and a carrier protein solution can be prepared; S3, the prepared chelating agent solution is added to the carrier protein solution, the carrier protein solution is put in a constant-temperature water bath and stirred uniformly for constant-temperature water bathing, and amixed solution can be obtained; S4, a semipermeable membrane is homemade; S5, arsenic ions and other heteroions which are not chelated are removed through pre-purification; S6, unbound carrier proteinis removed. Part of articles are homemade in the purification process, so that the purification cost can be notably reduced, the requirements for working environments are lower, the applicable rangeis wider, the purification time is effectively shortened by use of a combined chromatographic column, and the cost is reduced to a certain extent.