7 results about "High-performance liquid chromatography" patented technology

The invention specifically discloses a tea tree MYB transcription factor CsAN1 and an application thereof in regulation of anthocyanin metabolism. A nucleotide sequence of the tea tree MYB transcription factor CsAN1 is represented as SEQ ID NO:1. CsAN1 is connected with a 35S promoter and then is connected to a pBI121 carrier, meditated transformation of tobaccos is realized by agrobacteria GV3101, meanwhile, the gene as well as genes CsGL3 and CsTTG1 capable of forming compounds with the gene is used for transforming the tobaccos respectively, leaves of overexpressed tobacco plants turn red, anthocyanin content detected by a spectrophotometer is remarkably increased, and two main pigments including cyanidine and delphinidin are detected through HPLC (high performance liquid chromatography).

The invention discloses a sample pre-treatment method for detecting squalene contained in cigarette smoke through a high performance liquid chromatography method. The sample pre-treatment method aims at a tobacco leaf sample, trichloromethane is adopted as extraction solvent, soxhlet extraction is combined and adopted, and high extraction efficiency is guaranteed. Based on the sample pre-treatment method, the high performance liquid chromatography detection method for detecting squalene in smoke can be established, the chromatographic peak is good in separation, and the sample pre-treatment method has the advantages of being accurate, rapid, high in sensitivity, good in reproducibility, high in recovery rate and the like.

The invention provides a method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues. The taxifolin active compound is separated and purified from ampelopsis grossedentata tissues by using silica gel column chromatography and semi-preparative high performance liquid chromatography; the structure of the taxifolin is identified as taxifolin (4.6 mg) through LC-HR-ESI MS, 1D and 2D NMR spectrum data analysis, and the purity of taxifolin is analyzed to be larger than or equal to 98% through HPLC detection. The method is adopted for efficiently and economicallyseparating, purifying and identifying the taxifolin (1) monomeric compound from the old ampelopsis grossedentata leaves and the defective ampelopsis grossedentata leaves, the old ampelopsis grossedentata leaves and the defective ampelopsis grossedentata leaves are fully utilized, ampelopsis grossedentata resource waste is avoided, huge economic benefits are created, and ampelopsis grossedentata industry development is promoted.

The present invention belongs to the field of biology technology, and is method of increasing the TIAs content in hairy root of catharantus roseus. The present invention includes cloning str and g10h genes from catharantus roseus, constructing plant expression vector of str and g10h genes, genetically transforming to catharantus roseus to obtain transgenic catharantus roseus hairy root, determining the TIAs content in hairy root of catharantus roseus in high efficiency liquid chromatography process, and determining the expression of biological synthesized gene of TIAs in hairy root of catharantus roseus via fluorometric quantitative PCR. The transgenic catharantus roseus hairy root has obviously raised TIAs content, especially 234 times raisedVinblastine content. The present invention provides one new method of raising TIAs content in hairy root of catharantus roseus and producing Vinblastine and Vincristine as important clinical anticancer medicine.

The invention discloses a preparation method of halogenated aromatic sulfonyl chloride. The preparation method specifically comprises the following steps of: 1, preparing a SO2 water saturated solution; 2, preparing a heavy ammonium salt; and 3, preparing sulfonyl chloride, finally desolventizing the filtrate at 30-38 DEG C under reduced pressure by using a water pump until no liquid is discharged to obtain 6.0 g of brownish red liquid, controlling the purity to be 93.2% by using HPLC (High Performance Liquid Chromatography), and standing overnight to obtain a solid on the second day. The thionyl chloride used in the method is used as a raw material, the method is economical, cheap and easy to operate, the whole reaction process is safe and stable, the reaction efficiency is high, the pure product rate of the obtained product is high, and industrial large-scale production is easy.

The invention discloses a purification and impurity removal method of a lotus plumule extraction solution. The purification and impurity removal method comprises the following steps: 1) taking a proper amount of lotus plumule and extracting with an ethanol water solution; filtering an extraction solution and recycling ethanol from filtrate; adding a proper amount of purified water to prepare a medicine extraction solution; 2) taking a proper amount of chitosan and preparing into a chitosan clarifying agent; 3) adding a proper amount of the chitosan clarifying agent into the medicine extractionsolution and purifying and removing impurities; filtering to obtain a clarified solution; 4) carrying out further purification on the clarified solution and separating out an alkaloid type substance;detecting the content of an effective component neferine by utilizing high performance liquid chromatography, so as to calculate the retention rate of the effective component. Under the technologicalconditions that the ratio of the medicine extraction solution of the lotus plumule is 1 to 5, the adding amount of the chitosan is 1.4g/ml, the acting temperature is 60 DEG C and the pH (Potential ofHydrogen) value is 6.0, the retention rate of the neferine is maximum. The purification and impurity removal method has the advantages of simple technology, simple steps and convenience for operation; a toxic organic solvent is not used so that the purification and impurity removal method has no dangers to environment and human bodies and no solvent residues, and is suitable for large-scale industrial production.