Method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues

A taxifolin, separation and purification technology, applied in the field of research, development and utilization of vine tea resources, can solve the problems of waste of vine tea resources, underutilization of flavonoid active ingredients, etc., to create economic benefits, promote the development of vine tea industry, The effect of low economic cost

Pending Publication Date: 2019-12-03
湖北省农业科学院中药材研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology helps crush down large amounts of green teas that are discarded after being used over time without any useful properties like taste. It also allows us to isolate specific chemical components within these materials while maintain their original quality through extraction techniques such as solvent evaporation technique. Overall this innovation improves the efficiency and economy of producing new types of teacups with improved nutritional value compared to older methods involving burning them away.

Problems solved by technology

This patented technical problem addressed in this patents is how to efficiently use or extract valuable compounds contained within vinnettea leafs during processing into products like beverage called wagerings made up primarily of these essential nutrients.

Method used

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  • Method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues
  • Method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues
  • Method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues

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Embodiment 1

[0038] A method for isolating and purifying taxifolin from rattan tea tissue, characterized in that the steps are as follows:

[0039] a. Collect the old leaves and defective leaves of vine tea, and extract the total flavonoids crudely by ultrasonication with ethanol;

[0040] b. Obtain the crude flavonoid extract according to step a and separate it by normal phase silica gel column chromatography to obtain a component containing the target active ingredient;

[0041] c. Separating and purifying the component containing the target active ingredient by semi-preparative high performance liquid phase to obtain the monomer of the active compound;

[0042] d. Analyze the monomer structure of the target compound based on LC-HR-ESI MS, 1D and 2D NMR spectral data.

[0043] In step b, mix the total flavonoids extraction extract with 100-200 mesh normal phase silica gel and mix the sample in equal amounts, pack 5 times the mass of 300-400 mesh normal phase silica gel into a column, numbe

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Abstract

The invention provides a method for separating, purifying and identifying taxifolin from ampelopsis grossedentata tissues. The taxifolin active compound is separated and purified from ampelopsis grossedentata tissues by using silica gel column chromatography and semi-preparative high performance liquid chromatography; the structure of the taxifolin is identified as taxifolin (4.6 mg) through LC-HR-ESI MS, 1D and 2D NMR spectrum data analysis, and the purity of taxifolin is analyzed to be larger than or equal to 98% through HPLC detection. The method is adopted for efficiently and economicallyseparating, purifying and identifying the taxifolin (1) monomeric compound from the old ampelopsis grossedentata leaves and the defective ampelopsis grossedentata leaves, the old ampelopsis grossedentata leaves and the defective ampelopsis grossedentata leaves are fully utilized, ampelopsis grossedentata resource waste is avoided, huge economic benefits are created, and ampelopsis grossedentata industry development is promoted.

Description

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Claims

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Application Information

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Owner 湖北省农业科学院中药材研究所
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