16 results about "Chromatographic column" patented technology

The invention discloses a PR-HPLC (phase reversing-high performance liquid chromatography) determination method for simultaneously determining contents of five amino acids in earthworm injection. The method is characterized by comprising the following steps: preparing chromatographic conditions: a C18 or C8 phase reversing chromatographic column, column temperature: 37 DEG C, a detection wavelength: 248nm, gradient elution with flow rate of 0.8ml/min, a flowing phase A is 0.7% trifluoroacetic acid aqueous solution of 5.0mmol/L heptafluorobutyric acid, a flowing phase B is a 60% acetonitrile aqueous solution, and the flow rate of elution solution is as follows: 0 min 100%A, 0.5 min 98.5%A, 10min 90%A, 15min 67%A, 20min 20%A and 25min 0%A; preparing a reference solution and a test solution; and respectively measuring 10 microliters of reference solution and 10 microliters of test solution, respectively injecting the reference solution and the test solution into a phase reversing liquid chromatography, and carrying out the phase reversing liquid chromatographic determination and analysis. The determination method is simple and rapid in operation, and no derivating agent is needed; moreover, the stability is high, the repeatability is good, and the active ingredients and quality of the earthworm injection can be better controlled.

The invention relates to a preparation technology of a high-purity crude drug in pharmaceutics. The preparation technology comprises the steps of firstly, adding water into a breviscapinun crude product with more than 85% of scutellarin sold in the market for wetting, adding basic amino acid water solution to prepare water solution which has the pH value being 7.5-9.0 and includes 10mg-150mg/ml of breviscapinun; secondly, centrifugally clearing and filtering, taking filtrate to a macroporous resin chromatographic column taking polyacrylate as a substrate; and thirdly, adding a converting agent organic acid into collecting liquid, reducing scutellarin salt into scutellarin, naturally settling, discharging supernatant liquor, drying to obtain the high-purity scutellarin crude drug with the purity being over 99%. According to the preparation technology, as the organic solvent is not needed for recrystallization, the technology is continuous, automatic and large-scale; and more importantly, the organic solvents easy to burn and explode are avoided, and the high-purity scutellarin crude drug prepared by the preparation technology conforms to the industry policy of the national safety production.

The invention relates to a method for continuously preparing oligomeric proanthocyanidins. The method comprises the following steps: (1) preparing a catalytic degradation solution; (2) catalytic degradation: loading the degradation solution on a polyamide chromatographic column, and collecting effluent I; (3) membrane separation: enabling the effluent to pass through a membrane, and respectively collecting trapped fluid and permeate; (4) enriching: loading the permeate on the column to macroporous adsorption resin until saturation, replacing macroporous adsorption resin with the same specification and type to continue loading on the column, circularly and alternately using more than two resins, driving and removing impurities by using water, then performing desorbing by using alcohol water, and respectively collecting and combining the eluate II on the column and the desorption solution; (5) recycling: combining the effluent II in the step (4) with the trapped fluid in the step (3), adding a raw material containing high polymer proanthocyanidins for dissolving, recycling dissolved material as a supplement of the degradation solution in the step (1), and continuing to add on the column according to the method in the step (2); and (6) concentrating and drying: concentrating and drying the desorption solution obtained in the step (4) to obtain an oligomeric proanthocyanidins product.

The invention provides a method for extracting ethyl vanillate from peony. According to the method, peony petals are used as a raw material. Through heating reflux, an active component containing ethyl vanillate are extracted from peony petals; and through extraction, macroporous resin column chromatography, silica gel column chromatography and recrystallization, ethyl vanillate is obtained. By the method for extracting ethyl vanillate from peony, ethyl vanillate with high purity can be obtained, and the purity can reach 95% and above. During the extraction process of ethyl vanillate, medicines used in the process are all common medicines, and production cost is low. Meanwhile, organic solvents such as ethanol and macroporous resin in the chromatographic column can be repeatedly used. Thus, the environment is not polluted, and production cost is further reduced. The method of the invention is suitable for large-scale promotion and application. The ethyl vanillate obtained by the method has stronger activity than orlistat and can be applied in preparation of diet pills or a medicine with inhibitory effect on lipase activity.

The invention provides a determination method of polyethylene glycol 4000 content in compound polyethylene glycol electrolyte powder. According to the method, a high-performance liquid chromatographicmethod and a differential refractive index detector are adopted, a hydrogel chromatographic column, namely a Plaquagel-OH column is used as the chromatographic column, an appropriate salty solution is selected to serve as a mobile phase, and an isocratic elution mode is adopted. The method is convenient and feasible to use, high in precision, accurate in detection result and good in repeatabilityand recovery rate; and through verification, the method can be used for detecting the polyethylene glycol 4000 content in the compound polyethylene glycol electrolyte powder.

The invention discloses a chromatographic column device capable of rapidly increasing and reducing temperature. The device comprises a thermal insulation container (1), a front door (2), a heating isolation cover (10), a chromatographic column heating wire (9), a chromatographic column (8), and a heat-resistant support (5); the thermal insulation container (1) is a cubic and is provided with an openable front door (2); the heating isolation cover (10) is detachably attached to an inner surface of the heat insulation container (1), the heat-resistant support (5) is arranged inside the heating isolation cover (10), and fans are arranged at the top and the bottom of the heat-resistant support (5) to cool the chromatographic column (8); the chromatographic column (8) is spirally wound outsidethe heat-resistant support (5), and the chromatographic column heating wire (9) is spirally wound outside the chromatographic column (8) to heat the chromatographic column. Through the chromatographiccolumn device disclosed by the invention, the uniform and rapid temperature rise and temperature drop of the chromatographic column can be realized, and the device is suitable for gas chromatographyinstruments for rapid detection.

The invention provides a determination method of L-anserine, the detection method mainly comprises optimization of mass spectrum conditions, selection of a chromatographic column, optimization of a mobile phase, and preparation of a standard solution and a test solution, the determination method is a liquid chromatography and mass spectrum combined method, and formic acid and acetonitrile are used as the mobile phase. According to the determination method, through optimization of a test sample preparation method and improvement of test conditions, compared with an existing determination method, the determination method is higher in precision, durability and accuracy, the content of the L-anserine in the skipjack inulin tablet candies can be rapidly and accurately obtained, and the quality of the candies is further guaranteed.

The invention belongs to the technical field of biology and particularly relates to a method for purifying virus antigens, which is characterized in that the purification method comprises the step of anion exchange chromatographic column purification. The purification method has the advantages of realizing the purification of the virus antigens, greatly reducing the content of impurity protein and residual DNA of a finished product of a vaccine and producing virus antigens having good immunogenicity and high recovery rate and greatly improving the safety of the finished product of the vaccine under a production scale, along with mild process condition, good repetitiveness and suitability for large-scale industrial production.

The present invention is hygroplasm combining detection method for detecting several kinds of drugs in urine. The present invention features that based on practical sample condition and required detection sensitivity, the sample is first pre-treated via solid phase extraction, direct deposition or direct sample feeding, and then gradient eluted in linked chromatographic column with SKF525A as internal reference and water or methanol containing formic acid as flow phase. The method is simple and reliable in the quantitative detection of 19 kinds of drugs and has its methodologic indexes meeting the practical requirement.

The invention discloses a novel method for purifying an arsenic chelating type immune complex. The method comprises steps as follows: S1, a chelating agent solution is prepared firstly through dissolution of a chelating agent in a solvent; S2, a proper amount of carrier protein is taken and added to a borate buffer solution, and a carrier protein solution can be prepared; S3, the prepared chelating agent solution is added to the carrier protein solution, the carrier protein solution is put in a constant-temperature water bath and stirred uniformly for constant-temperature water bathing, and amixed solution can be obtained; S4, a semipermeable membrane is homemade; S5, arsenic ions and other heteroions which are not chelated are removed through pre-purification; S6, unbound carrier proteinis removed. Part of articles are homemade in the purification process, so that the purification cost can be notably reduced, the requirements for working environments are lower, the applicable rangeis wider, the purification time is effectively shortened by use of a combined chromatographic column, and the cost is reduced to a certain extent.