The invention discloses a method for measuring the residue amount of pyraclostrobin in grapefruits through a high performance liquid chromatograph. The method includes the steps that a
standard solution is prepared, and by adjusting detecting conditions of the high performance liquid chromatograph, a linear standard working equation of the pyraclostrobin is obtained; then
grapefruit peel, grapefruit pulp and whole grapefruit samples are
cut up respectively and subjected to homogenate to be extracted with
acetonitrile, and the product is subjected to
salting out and florisil-chromatographic-column purification, and is eluded with mixed liquid of
acetone and normal
hexane, wherein the volume ratio of the
acetone to the normal
hexane is 1:9; eluded liquid is concentrated to fix the volume with
chromatography acetonitrile, filtering is carried out, and the product is measured through an
ultraviolet detector of the high performance liquid chromatograph, and is quantified with the base-material external standard method. Experiments confirm that the sensitivity,
accuracy and precision of the method meet the technical requirement of
pesticide residue analysis, and the method has the advantages of being easy to operate, easy to popularize and grasp, low in cost, good in
repeatability and accurate in qualitative and quantitative effect.