Method for measuring residue amount of pyraclostrobin in grapefruits through high performance liquid chromatograph

A technology of high performance liquid chromatography and pyraclostrobin, which is applied in measurement devices, instruments, scientific instruments and other directions to achieve the effects of good repeatability, simple operation, and easy popularization and mastery.

Inactive Publication Date: 2018-09-28
HUNAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports on the determination method of pyraclostrobin residues in pomelo at home and abroad.

Method used

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Experimental program
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Effect test

Embodiment 1

[0039] The method for determining the residual amount of pyraclostrobin in grapefruit based on liquid chromatography-ultraviolet detector provided by the present invention comprises the following steps:

[0040]Step 1: Select high-performance liquid chromatography according to the properties of pyraclostrobin. The detection conditions are as follows: the detector adopts ultraviolet detector; 18 Chromatographic column, the column temperature is 30°C, the flow rate is 0.6mL / min, the injection volume is 20uL, the mobile phase is acetonitrile and water, the volume ratio of acetonitrile and aqueous solution is 70:30, the wavelength is 278nm, and the retention time is 12.6min;

[0041] Step 2: Preparation of standard solution

[0042] Accurately weigh 0.1030 g of pyraclostrobin standard substance with a mass concentration of 97.0% in a 100 mL volumetric flask, dissolve it with chromatographic acetonitrile and prepare a standard mother solution with a mass concentration of pyraclostr...

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Abstract

The invention discloses a method for measuring the residue amount of pyraclostrobin in grapefruits through a high performance liquid chromatograph. The method includes the steps that a standard solution is prepared, and by adjusting detecting conditions of the high performance liquid chromatograph, a linear standard working equation of the pyraclostrobin is obtained; then grapefruit peel, grapefruit pulp and whole grapefruit samples are cut up respectively and subjected to homogenate to be extracted with acetonitrile, and the product is subjected to salting out and florisil-chromatographic-column purification, and is eluded with mixed liquid of acetone and normal hexane, wherein the volume ratio of the acetone to the normal hexane is 1:9; eluded liquid is concentrated to fix the volume with chromatography acetonitrile, filtering is carried out, and the product is measured through an ultraviolet detector of the high performance liquid chromatograph, and is quantified with the base-material external standard method. Experiments confirm that the sensitivity, accuracy and precision of the method meet the technical requirement of pesticide residue analysis, and the method has the advantages of being easy to operate, easy to popularize and grasp, low in cost, good in repeatability and accurate in qualitative and quantitative effect.

Description

technical field [0001] The invention relates to the detection of pesticide residues, in particular to a method for measuring pyraclostrobin residues in pomelo by using high performance liquid chromatography (HPLC). Background technique [0002] Pyraclostrobin English common name: pyraclostrobin, its chemical name is N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxy Methyl carbamate, the chemical structure is as figure 1 Shown, chemical molecular formula: C 19 h 18 C l N 3 o 4 , relative molecular mass: 387.82, ADI value 0.03mg / kg bw, developed by BASF, Germany, is a methoxyacrylate fungicide with a pyrazole structure, and is a mitochondrial respiration inhibitor, that is, through the cell Prevents electron transfer during pigment synthesis. It has the functions of protection, treatment and leaf penetration. It has a wide bactericidal spectrum, has good activity on ascomycetes, basidiomycetes, half-knowledge fungi and oomycetes, and has a certain regul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 杨丽华熊峰龚道新罗海峰肖灿岭曾新颜
Owner HUNAN AGRI UNIV
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