27 results about "Cell" patented technology

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

The invention discloses a fluorescent probe responsive to both peroxynitroso anion and viscosity as well as preparation and application thereof. The structural formula of the fluorescent probe is as shown in a formula I which is described in the specification. The preparation method of the fluorescent probe provided by the invention is simple, and the fluorescent probe can be obtained only by a short reaction route. The fluorescence of the probe is weak, ONOO<-> and viscosity in a solution and a cell environment can be respectively detected under different excitation/emission channels, and the probe can be used for preparing ONOO<-> concentration and viscosity detection reagents. The probe is good in detection effect and high in anti-interference capability, and a novel potential powerful analysis tool is provided for researching the biological relevance between ONOO <-> and viscosity.

The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

The invention discloses an application of GSK1838705a in ALK + ALCL and crizotinib-resistant ALK + ALCL. GSK1838705a is used for treating tumors, GSK1838705a is used for treating ALK + ALCL, and GSK1838705a is used for treating ALK + ALCL of crizotinib-resistant patients. GSK1838705a can inhibit the proliferation of ALK + ALCL cell line and crizotinib-resistant ALK + ALCL cell line, and induces the apoptosis of the cell line. Compared with the blank control group, GSK1838705a significantly inhibits the expression of p-IGF, p-ALK, p-AKT, p-STATT3 in the ALK + ALCL cell line and the crizotinib-resistant ALK + ALCL cell line, and the expression of activated apoptosis-related proteins is increased significantly. GSK1838705a significantly inhibits the growth of transplanted tumors in mice, andimproves the survival time of transplanted tumor mice, so that GSK1838705a shows anti-tumor effects in the in vivo and in vitro experiments, and a new use for ALK + ALCL and crizotinib-resistant ALK +ALCL is provided.

The invention discloses a kit and a manufacturing method thereof. The kit is used for extracting genome DNA and comprises cell lysis buffer, nano magnetic beads and cleaning fluid, wherein the nano magnetic beads are Fe3O4 of silicon dioxide, the diameter of the nano magnetic beads is 200-500nm, and the cell lysis buffer comprises red blood cell lysis buffer and white blood cell lysis buffer. Thekit and the manufacturing method thereof are simple to use, easy to operate, safe, non-toxic, few in components, low in using cost, applicable to extraction of various animal tissues, high in DNA yield and quite wide in application prospect.

Real-time data from cells is recorded during operation of an electrolyzer. Synthetic data is generated based on historical data of the electrolyzer and the cells, the synthetic data comprising synthetic cell voltages and synthetic product output flow, synthetic anolyte pH, feed brine pH, or oxygen in chlorine gas concentration of the electrolyzer. Cell-specific k-factors or U₀ are determined from the historical data. A slow contamination is detected when a difference between the synthetic product output flow, synthetic anolyte pH, feed brine pH, or oxygen in chlorine gas concentration and a real-time product output flow, anolyte pH, feed brine pH, or oxygen in chlorine gas concentration exceeds a first threshold. A fast contamination is detected when cell-specific k-factors or U₀ exceed a second threshold and a trend of a difference between the synthetic cell voltages and real-time cell voltages or a derivative of the difference meets or exceeds a conditional logic rule.

A treatment tool may generate and transmit a predetermined bioactive frequency and/or waveform of electromagnetic energy and/or acoustic energy into a given region of treatment for inducing a particular cellular outcome/behavior of at least one targeted cell selected from the region of treatment. The region of treatment may be at least a single organism or portion thereof. The region of treatment may include other different types of cells in addition to the at least one targeted cell. The treatment tool may be used in vivo, non-invasively, and selectively, wherein the particular cellular outcome/behavior occurs in the at least one targeted cell but not other cells. The treatment tool may have a transmitter assembly, an antenna tuner, an attenuator, a broadband power amplifier, and a frequency/waveform generator; and the treatment tool may also have a computer. The treatment tool may be used to treat cancers and/or other conditions.

A carrier includes a supporting part on which a specific number of cells A are supported. The cells A contain a specific number of copies of a nucleic acid, and the supporting part is made of a water-decomposable material.

The invention provides a cow interdigital skin explant model construction method, and belongs to the technical field of skin explant models. The model construction method comprises the following steps: immersing the interdigital skin of the dairy cow into a transportation culture medium for transportation, cleaning the interdigital skin of the dairy cow by using a washing culture medium after transportation is completed, and culturing the cleaned interdigital skin of the dairy cow in a cell chamber containing a tissue culture medium to complete model construction; and finally, carrying out feasibility evaluation on the cow interdigital skin explant model. The constructed 3D dairy cow interdigital skin model conforms to experimental animal 3R principles of reduction, substitution, optimization and the like, meanwhile, the constructed model can maintain good tissue structure integrity, the dairy cow interdigital skin in-vivo environment is fully simulated, and the method has great significance in deep research of dairy cow interdigital skin pathogenic microorganism invasion action mechanism.

The invention relates to establishment and improvement of a preoperative evaluation and prediction model for satisfactory tumor cell deduction of advanced ovarian cancer. Herein, improved modeling is carried out on the basis of the Sduidan score table; and serological HE4 and ROMA indexes are added, the HE4 calculated according to the data is larger than 264.7 pmol/L, the CA125 calculated according to the data is larger than 545.6 U/ml, the ROMA indexes calculated according to the data are larger than 90.0%, and the prediction scores are given to be one score respectively; and then two different radiology department high-age doctors perform secondary radiology reading scoring to calculate predicted scores of all cases; and finally, an ROC curve is drawn according to a prediction score and an operation result to obtain an area AUC under a subject working curve, and evaluation of the model is carried out to predict the clinical value of the unsatisfactory tumor cell deduction operation of the advanced ovarian cancer patient. The prediction value of the prediction model is higher than that of a Suidan prediction model. When the model is used for predicting the dissatisfactory tumor cell deficit operation, the sensitivity is obviously higher than that of a Suidan score table, which provides a basis with a certain prediction value for clinicians to judge whether the dissatisfactory tumor cell deficit operation can be performed or not.

The invention provides monocytes expressing CD16 and CD163 and experimental system for drug screening or evaluating drug candidates where the modulation of CD16 and CD163 is desired.

The invention discloses a cell tracer with low toxicity and a high cell uptake rate as well as a preparation method and application of the cell tracer. The cell tracer is a PAA and PEG coated ultra-small superparamagnetic iron oxide nanoparticle. The cell tracer is obtained by synthesizing an ultra-small superparamagnetic iron oxide nanoparticle through a thermal decomposition method and then sequentially coating PAA and PEG. The cell tracer with the low toxicity and the high cell uptake rate is obtained by coating the ultra-small superparamagnetic iron oxide nanoparticles with the PAA and thePEG, and can be used as a cell tracer applied to nuclear magnetic resonance imaging; the cell tracer can be used for efficiently marking human adipose tissue-derived stromal cells without using a transfection reagent, has no obvious toxicity to the human adipose tissue-derived stromal cells and induced pluripotent stem cells, and can be used for tracing the human umbilical cord mesenchymal stem cells transplanted into a brain of a rat.