Application of FXYD3 protein or coding gene thereof as target spot in preparation of medicine for preventing and treating psoriasis

[0029](1) Immunofluorescence detection of the expression of FXYD3 protein (GeneBank number: NP_032583.1) in the skin

[0030] Selected from outpatients of the Department of Dermatology of the Second Affiliated Hospital of Zhejiang University, with typical clinical manifestations of psoriasis patients (29 cases) (Human Research Ethics Committee of the Second Affiliated Hospital of Zhejiang University School of Medicine, project acceptance number: Research 2021-0268, event acceptance No.: IR2021001150), in which 2 cases of skin lesions were taken by conventional method and placed in normal saline, the samples were processed on the same day, and pathological sections were prepared; after paraffin sections were dewaxed, antigen retrieval was carried out with 0.01M (pH=6.0) sodium citrate; 3%H 2 o 2 Endogenous peroxidase was blocked, non-specific proteins were blocked with blocking solution; primary antibody (FXYD3 antibody, abcam, ab205534) was incubated overnight at 4°C, fluores...

[0035] (1) Preparation of skin lesion samples from patients with psoriasis

[0036] Selected from outpatients of the Department of Dermatology of the Second Affiliated Hospital of Zhejiang University, with typical clinical manifestations of psoriasis patients (29 cases) (Human Research Ethics Committee of the Second Affiliated Hospital of Zhejiang University School of Medicine, project acceptance number: Research 2021-0268, event acceptance No.: IR2021001150), wherein 29 cases of skin lesions and 16 cases of normal skin samples at the edge of skin lesions were placed in normal saline by conventional methods, and the samples were processed on the same day to prepare pathological sections. ).

[0037] (2) Immunohistochemical staining of human and mouse skin tissues

[0038] Human and mouse skin tissues were obtained, fixed in 10% formalin, embedded in paraffin within one week, and sectioned. The paraffin sections were placed in an oven at 67°C for 2 hours, dewaxed to water, an...

[0054] Construct transgenic mice with keratinocyte-specific knockout of the FXYD3 gene (as shown in SEQ ID No.21) through the Cre / loxp recombination system: FXYD3 flox / flox Mice with K14 cre The transgenic mice were cross-breeded, and after identification, the K14 cre FXYD3 flox / flox Mice were used as cKO experimental mice, littermate FXYD3 flox / flox Mice were used as WT experimental mice. Among them, FXYD3 flox / flox Mice and K14 cre The mice were all of C57BL / 6 background, purchased from Nanjing University-Nanjing Model Animal Center, and bred in the Experimental Animal Center of Zhejiang University.

[0055] (1) After IMQ was applied to the back skin of mice, back imaging was performed on 3 and 5 days. Psoriasis severity was assessed by mouse skin thickness, erythema, and scaling. Measure the mouse ear thickness with a caliper. Scale and erythema were scored on a scale of 0-4: 0, none; 1, mild; 2, moderate; 3, marked; 4, very marked.

[0056] (2) Skin sections were...