4 results about "Experimental animal" patented technology

The invention relates to a method for establishing an animal model for senile dementia, a special liquid medicine and a dosing device, and belongs to the technical field of neurosciences. The method comprises the following steps: obtaining positioning coordinates of paraceles according to brain atlases or/and magnetic resonance image data of an experimental animal; respectively burying a conduit of the dosing device in left and right paraceles of the animal, and injecting the special liquid medicine to the paraceles at a constant speed in 14-16 minutes under a sterile environment within 2-3 weeks, wherein the liquid medicine amount is 100-250 ml methanol per day; alternately dosing the left and right paraceles, wherein the dosing frequency is 18-30 hours at an interval; and continuously dosing for 2-10 months to obtain a non-human primate animal model for senile dementia. With the adoption of a novel dosing mode: dosing to the paraceles at the constant speed, medicine can circulate to whole brain through the cerebrospinal fluid to exert the toxic effect, so that peripheral damage by the medicine is avoided. The AD model is established by injecting methanol or formaldehyde to the paraceles, so that behaviors and pathological symptoms are typical, the basic physiological status is good and the individual difference is relatively smaller.

The invention discloses an application of amomum tsao-ko essential oil, an application of the amomum tsao-ko essential oil in preparing drugs for the treatment or prevention of brain dysfunction and other disorder disease and an application of the amomum tsao-ko essential oil in preparing health products for improving brain function and disorder diseases. For the first time, the amomum tsao-ko essential oil is found to have a new use in medicine or large health products that show activity in brain function and disorder diseases of experimental animals, and for the first time, the amommum tsao-ko essential oil or arbitrary components containing the amommum tsao-ko essential oil can improve brain function such as Alzheimer's disease, depression and anxiety in animal body models and the symptoms of disordered animals; compared with traditional medicine for treating the brain function and disorders, the amomum tsao-ko essential oil or the arbitrary components thereof has the mechanism of inhibiting the action of multi-molecular synergy of the cholinesterase and regulating the synergistic action of multiple molecules, such as neurotransmitters, on multiple targets, and is good in drug substitution and easy to penetrate the blood-brain barrier, and the advantages of toxicological safety, stable metabolism, long half-life period and small side effects are achieved.

The invention discloses a multifunctional experimental animal cage which comprises a cage body, a cage door, an object-placing groove, a feeding trough opening, a feeding trough, a water bottle, a fish tank and carrying bands, wherein the object-placing groove is formed in the top of the cage body, the cage door and the feeding trough opening are formed in the upper portion of the cage body, the feeding trough is formed in the feeding trough opening, the water bottle is arranged in the feeding trough, the fish tank is arranged in the object-placing groove, and the carrying bands are arranged on the cage body and the fish tank. Through the multifunctional experimental animal cage, various animals can be fed, the number of devices is reduced, space is saved, and feeding and use are convenient.

The invention provides a cow interdigital skin explant model construction method, and belongs to the technical field of skin explant models. The model construction method comprises the following steps: immersing the interdigital skin of the dairy cow into a transportation culture medium for transportation, cleaning the interdigital skin of the dairy cow by using a washing culture medium after transportation is completed, and culturing the cleaned interdigital skin of the dairy cow in a cell chamber containing a tissue culture medium to complete model construction; and finally, carrying out feasibility evaluation on the cow interdigital skin explant model. The constructed 3D dairy cow interdigital skin model conforms to experimental animal 3R principles of reduction, substitution, optimization and the like, meanwhile, the constructed model can maintain good tissue structure integrity, the dairy cow interdigital skin in-vivo environment is fully simulated, and the method has great significance in deep research of dairy cow interdigital skin pathogenic microorganism invasion action mechanism.