The invention provides an application of a 24R-Pyxinol compound as a mitochondrial
fluorescence probe. The fluorescent probe can quickly target mitochondria of living cells. Compared with other
mitochondrial targeting agents, the dye is not influenced by the mitochondrial
membrane potential, and can still dye mitochondria when the mitochondrial
membrane potential is reduced or disappears; and meanwhile, the compound has
high selectivity on myocardial cells. Compared with MitoRed, the dye can dye mitochondria more quickly, and the
dyeing time is shorter. In addition, the probe also has the advantages of good
solubility, strong color development, good bio-compatibility and the like, and has a wide application prospect in the fields of fluorescent biomarkers and life science detection.