Application of 24R-Pyxinol compound as mitochondrial fluorescent probe

[0039] Example 1: Fluorescence imaging of probe 1 in cardiomyocytes

[0040] The H9C2 cardiomyocytes in the logarithmic growth phase were digested and counted, and the number was 8×10 3 Inoculate in 35mm laser confocal culture dish per 100 μL well, and culture in a cell incubator for 24 h; add fluorescent probe 1 to make the final concentration 1 μM, and continue to culture in the original culture environment for 1 h; discard the culture medium, wash 3 times with PBS, Observations were made under a confocal laser microscope. like figure 1 As shown, probe 1 emits bright green fluorescence in the cell, the aggregation point is extremely obvious, and the linear mitochondria in the cell are clearly visible.

[0041] Example 2: Fluorescence imaging of probe 2 in cardiomyocytes

[0042] The operation method is the same as in Example 1, only the corresponding probe 1 in Example 1 needs to be changed to probe 2.

[0043] like figure 2 As shown, probe 2 also emits bright green fluorescence in the cell, the aggregation point is extremely obvious, and the linear mitochondria in the cell are clearly visible.

[0044] Example 3: Fluorescence imaging of probe 3 in cardiomyocytes

[0045] The operation method is the same as in Example 1, only the corresponding probe 1 in Example 1 needs to be changed to probe 3.

[0046] like image 3 As shown, probe 3 also emits bright green fluorescence in the cell, the aggregation point is extremely obvious, and the linear mitochondria in the cell are clearly visible.