101 results about "Biological organism" patented technology

The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.

The invention belongs to the technical field of biology, and particularly relates to a characteristic polypeptide for identifying the deer-horn glue of Cervus nippon or Cervus elaphus, and application of the characteristic polypeptide. The characteristic polypeptide provided by the invention is as follows: a peptide fragment 1 is as shown in SEQ ID No. 1: ASTGAALVVR; and a peptide fragment 2 is as shown in SEQ ID No. 2: TPVGQGPSVPVPNR. The characteristic polypeptide and the detection method provided by the invention provide reference for searching a characteristic peptide fragment in related species lacking in a database. The characteristic polypeptide provided by the invention has excellent specificity and stability for the deer-horn glue of the Cervus nippon or the Cervus elaphus, is high in specificity, can be used for identifying deer-horn glue medicinal materials and Chinese patent medicines added with the the deer-horn glue medicinal materials, and has a good application prospect.

The invention provides a culture method for pleurotus eryngii, and relates to a technology of applying the stems of shiitake mushroom to pleurotus eryngii culture. Compared with the prior art, the method has the advantages that 1, the shiitake mushroom stems soaking solution is a pure natural nutritional solution containing various types of bioactive substances; the culture materials and the shiitake mushroom stems soaking solution are stacked and fermented to prompt the growth of hypha of pleurotus eryngii, namely, hypha grows well and is high in deformity resistance rate; 2, the shiitake mushroom stems soaking solution can rejuvenate the hypha after picking the first flush, which prompts the formation of primordium of pleurotus eryngii as well as the growth of the entity; the second flush and the second flush are large in size, high in quality and high in biological efficiency; 3, the problem that the quality of pleurotus eryngii is lowered down due to chemical synthesized fertilizer used for a long term is solved, thus the quality of pleurotus eryngii is improved, and meanwhile the output is increased; the total biological efficiency is raised by 40% by being compared with that of the control group; 4, fuel used for sterilizing at high temperature is saved, so that the energy is saved, and the shortage that the emission of carbon dioxide generated in traditional production of edible mushrooms is increased can be removed.

Methods and devices for microfluidic detection of a biological maker in a biospecimen collected from a subject are disclosed. The microfluidic devices include nanoparticle-based nanosensors comprising supramolecular recognition sequences, protease consensus sequences, post-translationally modifiable sequences, or sterically hindered benzylether bonds for specific interaction with a biological marker. Also disclosed are particular nanosensors for detecting cytokines, and other proteins based upon supramolecular recognition without chemical modification or enzymatic cleavage.

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.

The invention relates to a pulmonary tuberculosis variation activity marker, a kit, a method and a model construction method, and the pulmonary tuberculosis variation activity marker is characterized in that the pulmonary tuberculosis variation activity marker is a plasma protein biomarker in a blood sample, and the plasma protein biomarker is a combined marker comprising one or more of C1QB protein and CCL19 (MIP3B) protein; a combined marker of C1QB and CCL19 (MIP3B) is used as a biomarker for detecting the activity of pulmonary tuberculosis for the first time, a rapid diagnosis model for detecting the activity of pulmonary tuberculosis is constructed, a new direction is provided for clinical diagnosis of tuberculosis, and as a technical conception for diagnosing the activity of pulmonary tuberculosis, the biomarker provides a new target spot for the diagnosis of the active tuberculosis; therefore, the invention overcomes the defects of low detection rate, long time consumption and the like of the existing active tuberculosis diagnosis, has the characteristics of good specificity and high sensitivity, and has good clinical application value for auxiliary diagnosis of tuberculosis activity.

The invention discloses lactobacillus plantarum capable of adjusting ampicillin induced intestinal flora disturbance and belongs to the technical field of food. The lactobacillus plantarum CGMCC12436disclosed by the invention is amplification cultured under assistance of skimmed milk, trehalose and saccharose; after being frozen and dried, the lactobacillus plantarum is prepared into a biologicalpreparation; a viable bacterial content of the lactobacillus plantarum CGMCC12436 in the preparation is larger than 109CFU/g. The lactobacillus plantarum CGMCC12436 disclosed by the invention has theadvantages of lower growth generation time, high survival rate in in-vitro simulated gastric fluid and intestinal juice, good adhesion on human HT-29 cells and relatively lower drug resistance in drug allergy testing. The biological preparation prepared from the lactobacillus plantarum has the advantages that ampicillin induced intestinal flora disturbance in a mouse body can be obviously recovered, intestinal functions can be improved and has good market application prospect.

The invention discloses a preparation method of traditional Chinese medicinal submicron powder for animals. The method comprises the following steps: 1, cleaning ; 2, slicing: cutting crisp and frangible traditional Chinese medicines into thick slices or long segments, and cutting solid or high-toughness traditional Chinese medicines into slices or short segments; 3, medicinal material division: dividing the above sliced medicinal materials into high temperature resistant traditional Chinese medicinal materials and traditional Chinese medicinal materials not resistant to high temperatures; 4, drying; 5, preliminary crushing: crushing the dried high temperature resistant traditional Chinese medicinal materials by adopting a hammer type crusher, and crushing the traditional Chinese medicinal materials not resistant to high temperatures by adopting an air-cooled universal crusher; and 6, micronization: adding the preliminarily-crushed traditional Chinese medicinal material particles into an ultrasonic airflow crusher, and further crushing to prepare the traditional Chinese medicinal submicron powder for animals. The method is in favor of reserving the bioactive components of the traditional Chinese medicines, and the prepared submicron powder for animals has the characteristics of high mixing uniformity, use convenience, high bioavailability and good palatability; and the method has the advantages of simple operation, low energy consumption, low emission, and low production cost, and is more suitable for the large scale production.

The invention discloses application of an Mg/Fe oxide modified biochar nano composite material in removing antibiotics, the Mg/Fe oxide modified biochar nano composite material takes biochar as a matrix, and Mg/Fe oxide is loaded on the surface of the biochar; the application method comprises the following steps: mixing the Mg/Fe oxide modified biochar nano composite material with ammonium persulfate and an antibiotic solution, and reacting to complete the removal of the antibiotics. The Mg/Fe oxide modified biochar nano composite material has the advantages of low price, high efficiency, large-scale production, high adsorption capacity, capability of quickly realizing solid-liquid separation, environmental friendliness and the like, is applied to activating persulfate to degrade antibiotic pollution in a water body, and is good in removal effect and high in reaction speed.

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.

The invention relates to a preparation method of a glowing nanometer fibre with the biocompatibility. The preparation method comprises the following steps of dissolving carbon nanometer particles in a solvent, adding a high molecular material under a stirring condition in the mass ratio of the high molecular material to the carbon nanometer particles being (0.5:1)-(1:2), and stirring for 1-3 hours so as to obtain a uniform spinning fluid ultimately; injecting a spinning liquid into a needle cylinder, regulating an electrostatic spinning technology, collecting a layer of uniform white fibres which are collected from a receiving aluminum foil; and putting the white fibres into a high-temperature pipe-shaped atmosphere furnace, inputting nitrogen, heating to 600-1000 DEG C, carrying out heat preservation for 1-5 hours, and finally cooling to the room temperature so as to obtain the glowing nanometer fibre containing the carbon nanometer particles. The obtained fibre has the good biocompatibility, meanwhile, the fibres have a glowing property, the glowing nanometer fibre is suitable for being used in a biomedical field, and the glowing nanometer fibre is particularly utilized as a bracket material and is convenient to anaphase observation. The preparation method provided by the invention is simple, the production cost is low, and the preparation method can further meet the production and application requirements.

The invention discloses a method for rapidly removing nitrate in a water body by using biochar immobilized denitrifying bacteria. The preparation method comprises the following specific steps: firstly, screening high-efficiency denitrifying bacteria to obtain a strain of pseudomonas stutzeri N3; and co-culturing the bacterial suspension of N3 with biochar, carrying out adsorption immobilization, and cleaning with normal saline to obtain the biochar immobilized denitrifying bacteria. The biochar is used as a carrier for immobilizing denitrifying bacteria, so that the method has the advantages of wide material source, simplicity in preparation, low cost, good treatment effect and the like, and resource utilization of wastes is realized. The biochar is used for immobilizing denitrifying bacteria, it is found that the removal effect on nitrate after immobilization is significantly better than simple biochar adsorption and denitrifying bacteria degradation. The method is of great significance for the rapid removal of nitrate in water bodies.

The invention discloses an insect-resistant fusion gene mCryAb-VIP3A for coding insecticidal protein and an expression vector and application thereof, and aims at solving the technical problem that the drug resistance trend of insects to an insecticidal preparation cannot be restrained. According to the invention, an insect-resistant gene and the insect-resistant fusion gene mCryAb-VIP3A are designed, the protein encoded by the insect-resistant fusion gene is also provided, and an expression vector constructed by the insect-resistant fusion gene and recombinant bacteria constructed by the expression vector are designed. The insect-resistant fusion gene is applied to preparation of insect-resistant plant cells. The protein encoded by the insect-resistant fusion gene is applied to preparation of an insect-resistant preparation. The insect-resistant fusion gene disclosed by the invention can be stably expressed in corn, so that the biotoxicity generated by singly transferring the originalVIP3A (a) gene into a plant is avoided, the gene silencing phenomenon existing in exogenous gene expression is overcome, more choices are provided for plant pest control, and a new method is providedfor restraining the drug resistance trend of insects to an insecticidal preparation.

The invention relates to the technical field of biological information, in particular to a method for analyzing and mining functional genes by combining genome three-dimensional structure difference identification and transcriptome gene expression level difference. The invention provides a genome three-dimensional structure difference identification method, which comprises identification of difference AB Compartment, identification of difference TAD and identification of difference Loop on the basis of HiC data, and can realize efficient identification of different levels of genome three-dimensional structure differences of animals and plants by using in-situ HiC data. The invention provides a method for analyzing and excavating functional genes by utilizing the genome three-dimensional structure difference identification method in combination with transcriptome gene expression level difference, and by utilizing the combined analysis method, difference analysis can be performed from two aspects of a genome three-dimensional structure and a transcriptome, so that efficient excavation of animal and plant functional genes is realized.

The invention discloses cell membrane-simulated surface-modified bacterial cellulose and a preparation method and application thereof. The method comprises the following steps: oxidizing bacterial cellulose with a periodate to obtain aldehyde-based bacterial cellulose; and reacting the aldehyde-based bacterial cellulose with a disubstituted choline phosphonate, adding a reaction product into an alkaline solution for hydrolysis, and performing washing with water and drying to obtain the cell membrane-simulated surface-modified bacterial cellulose. The phosphorylcholine-based bacterial celluloseprepared by the invention is non-toxic to cells under physiological environment, and has good anti-protein adhesion and anti-cell adhesion. The preparation method has mild conditions, is easy to operate, can obtain a cell membrane-simulated modified bacterial cellulose material with anti-bio adhesion on the surface, and is suitable for preparing anti-bio adhesion and adhesion-proof biomaterials.

The invention discloses application of miRNA 408 in regulating and controlling cadmium accumulation of crops, and belongs to the technical field of biology. The MIR408 precursor gene overexpression vector is constructed, receptor rice is transformed to obtain transgenic rice with the MIR408 function, on one hand, cadmium content accumulation of the rice can be remarkably inhibited, and on the other hand, other agronomic traits are not fundamentally influenced, so that an important genetic material can be provided for cultivating low-cadmium rice, and the application prospect is wide. The miRNA 408 can also be used for genetic transformation of other monocotyledonous crops, and provides reference for other crops planted in cadmium-contaminated soil to reduce heavy metal hazards such as cadmium accumulation.

The invention belongs to the technical field of forestry biology, and discloses a Plant growth promoter for promoting restoring, growing and regeneration of barks and a preparing method and application thereof. Through implementation of methods such as spraying, smearing and injection, the Plant growth promoter can promote length and thickness of the original barks and the regeneration of new barks, can restore damaged barks and maintain and grow old and new barks, and can prevent the damaged parts of the barks from forming scars, tree burls and hollow cavities. The Plant growth promoter is particularly applicable to landscapes, pavements, gardens and municipal administration.

The invention discloses a tetracycline (TC) degrading strain as well as a preparation method and application thereof. The TC degrading strain is Kluyvera intermedia, is preserved in the China GeneralMicrobiological Culture Collection Center (CGMCC) on July 15, 2020, and has a preservation number of CGMCC No.20117. The strain can be applied to preparation of a TC degrading microbial inoculum, is beneficial to enrichment of a strain resource library of TC degrading bacteria, and provides an effective biodegradation method for treatment of TC pollution. The used strain has high efficiency of degrading minocycline, and the degradation rate of the strain reaches 90% or above within 72 h under laboratory conditions and under the condition that the initial content of minocycline is 50 mg/L. An effective biological approach is provided for removing the minocycline in environmental water bodies.