The invention relates to the field of microorganisms and especially relates to a
bacterial strain, a construction method and an application thereof. The invention provides the
bacterial strain for high-yield
campesterol. An exogenous functional
gene DHCR7 is guided into a
chassis bacterial strain. Compared with
plant extraction, the method for utilizing two
yeast chassis bacterial strains including recombined brewer
yeast and lipid
yeast solution to produce
campesterol has the advantages of low cost, few side products and low
pollution; a feasible method is supplied for the industrial production of the
campesterol. The basis is established for the
biosynthesis of steride
hormone drugs including downstream progesterone, 4AD and the like. The bacterial strain has guidance significance in acquiring high-yield campesterol through other microorganisms.