38 results about "Toxin" patented technology

The invention relates to a method of preparing a vine tea mask capsule. The method comprises the steps of preparing vine tea powder, degreasing, extracting vine tea flavonoid, crystallizing and removing impurities, crushing and disinfecting, preparing mask capsule powder and finally filling the powder into a capsule. The vine tea mask capsule is composed of pure plant components, is antiseptic-free and additive-free, and causes no irritation and harm to skins. When a user is using the capsule, the user opens the capsule, fetches 80ML hot water with 80 DEG C, and slowly and uniformly pours thepowder in the capsule into the hot water while stirring. The user stirs the capsule powder to make the power fully dissolved and then pours into a mold plate. When the dissolved powder is cooled to be a mask, the user applies the mask onto the face for 20 to 30 minutes. The vine tea mask is prepared from pure natural konjac gum andxanthan gum, is crystal clear in appearance, and is fast in penetration and fine in absorption. No non-woven fabric needs to be a medium, and most essences are not liable to be absorbed by mask paper. Furthermore, various masks with beauty treatment efficacies can be prepared based on needs of skin whitening, skin dullness removal, rosy cheek improvement, acne clearance, toxin expelling or the like.

The invention discloses vegetable insecticide of soluble matrine special for killing plant louses and a preparation method thereof, which aims at providing the vegetable insecticide which adopts Chinese herbal medicine as raw material, has high matrine content and low toxin but high efficiency, is harmless to human and livestock as well as special for killing plant louses and the preparation method thereof. The invention comprises 11-13 portions of Chinese herbal medicine extract containing the matrine, 10-20 portions of emulsifier, 4-8 portions of penetrating agent, 0.1 portions of antioxidant and 57-64 portions of deionized water; the Chinese herbal medicine extract containing the matrine is the extract of the mixture by weight of 15-25 portions of sophora flavescens ait, 10-17 portions of chinaberry tree, 3-4 portions of argy wormwood leaf, 6-8 portions of celastrus angulatus maxim, 4-8 portions of derris, 2-4 portions of gardenia, 7-9 portions of herba artemisiae, 10-14 portions of gelsemium elegans, 5-7 portions of orange seed and 7-10 portions of Chinese wingnut leaf. The insecticide of the invention comprises the matrine with super high content about 1.3 percent and is special for killing the plant louses through scientific preparation of a plurality of Chinese herbal medicines and has powerful penetrating performance, high efficiency and insignificant toxin and is in favor of absorption by plants.

The invention discloses a medicament for treating cattle/pig constipation, and a preparation method thereof. The medicament is prepared from rhubarb, immature bitter oranges, magnolia officinalis, fructus cannabis, bunge cherry seeds, mirabilite, figwort roots and radix rehmanniae according to a certain mixture ratio by weight. The medicament has the efficacy in clearing heat, releasing toxin, purging pathogenic fire, dispelling fire stasis in large intestines, promoting blood circulation, removing blood stasis, relieving constipation, invigorating gallbladder and eliminating abdominal distension/pain, has the advantages of short treatment course, remarkable therapeutic effects, low cost, high cure rate and no relapse, and is widely used for treating cattle/pig constipation.

The invention discloses an O type foot and mouth disease virus-like particle vaccine as well as a preparation method and an application thereof, and belongs to the fields of genetic engineering and immunology, aiming at solving the problems of an existing inactivated foot and mouth disease vaccine which is poor in safety and unsatisfying in use effect. The amino acid sequence of the vaccine is shown as SEQ ID NO: 1 in a sequence list, and a nucleotide sequence for encoding the amino acid sequence is shown as SEQ ID NO: 2 in the sequence list. After being immunized, the vaccine can induce an animal to simultaneously generate an O type foot and mouth disease resistant antibody; the vaccine exists in the form of virus-like particles, and foot and mouth disease virus VP1 epitope locates on the surface of the virus-like particles. According to the invention, the amino acid sequence constituting the virus-like particle vaccine and the nucleotide sequence for encoding the amino acid sequence are provided, and the nucleotide sequence contains all genes of coat protein which can be self-assembled as the virus-like particles in expression. The preparation of the O type foot and mouth disease virus-like particle vaccine disclosed by the invention is good in safety and free from the probability of reversion or toxin dispersion; and the vaccine is long in immunizing validity period and convenient in transportation and storage.

The invention belongs to the technical field of prevention and treatment of plant diseases and relates to a bacillus amyloliquefaciens strain capable of preventing and treating wheat scab and application thereof. The preservation number of the separated bacillus amyloliquefaciens S76-3 is CCTCC NO:M2014315, and nucleotide of 16S rDNA of the separated bacillus amyloliquefaciens S76-3 is shown in SEQ ID NO:1. The invention discloses separation, identification and broad-spectrum bacteriostasis of the bacillus amyloliquefaciens, spore yield fermentation optimization of the bacillus amyloliquefaciens and field application of the bacillus amyloliquefaciens in prevention and treatment of wheat scab. The liquid inoculant of the bacillus amyloliquefaciens fermentation diluent can be used for effectively inhibiting the wheat scab and harm in the field and reducing toxins in wheat ears. The bacillus amyloliquefaciens strain has strong stress resistance and does less harm to the environment, so that the bacillus amyloliquefaciens strain can be taken as an efficient antibiological inoculant; and the bacillus amyloliquefaciens is broad in antibacterial spectrum and can be used for inhibiting growth of ten plant pathogenic fungi including fusarium graminearum, so that the bacillus amyloliquefaciens strain has a good development and application prospect.

The invention specifically relates to a method for estimating in-vitro detoxification effect of a mycotoxin detoxification agent by using liquid chromatography, belonging to the technical field of biological detection. The method comprises the following steps: (1) early stage preparation: I, drafting of a standard curve of mycotoxin; II, preparation of a sample of the detoxification agent; and III, acquisition of a reaction medium; (2) detoxification: I, evaluation of in-vitro adsorption detoxification; and II, evaluation of simulated in-vivo adsorption detoxification; and (3) liquid chromatographic detection. The method for estimating in-vitro detoxification effect of the mycotoxin detoxification agent by using liquid chromatography can simulate gastrointestinal environments, adopts supernatant of chyme in the alimentary canal of an animal as a reaction medium, completely reproduce the gastrointestinal environment in the animal and more authentically evaluate the adsorption effect of an adsorbent.

A method for the detection or determination of a target comprising a plurality of target compounds, or derivatised target compounds, said method comprising: immobilising said target on a carrier, directing radiation at said target, said radiation being selected to cause said target to emit a relevant radiation, detecting said relevant radiation emitted by said target, and analysing said detected radiation to identify and/or quantify the plurality of target compounds in said target.

The invention discloses a multifunctional organic fertilizer and a preparation method thereof. The multifunctional organic fertilizer comprises the following raw materials in parts by weight: 30-50 parts of silkworm litter, 3-8 parts of mulberry twig, 2-8 parts of pond sludge, 10-30 parts of lignite, 5-20 parts of grass carbon, 5-20 parts of rice bran, 3-10 parts of saccharomycete, 3-10 parts of Bacillus licheniformis and 50-300 parts of purified water. The organic fertilizer adopts a biological fermentation technology, and has the advantages of multiple functions, simple production process, low production cost, high fertilizer effect and quick action; the finished product contains a large amount of Bacillus licheniformis, Bacillus subtilis, Bacillus mucilaginosus, Bacillus megaterium, Bacillus amyloliquefaciens and actinomycete, has strong aerobic fermentation and decomposition capabilities, activates microorganism propagation, and can loosen the soil, enhance the soil productivity and adsorb residual heavy metal and other harmful toxin in the soil; and the organic fertilizer can be applied to various vegetables, fruit trees, Chinese medicinal herbs, tea, melons, fruits and the like.

The invention relates to a toxin-removed animal liver food which includes a complete and fresh toxin-removed animal liver, seasonings and spices. A complete and fresh liver is rapidly taken out of an animal body, the gall bladder is cut off, the first porta hepatis of the liver is rapidly dissected, a disinfected medical catheter is inserted into the portal vein, biliary tract and hepatic artery blood vessel; perfusate is filled, macrophages and other immune cells containing a large amount of toxins, blood, harmful substances and bile of the liver are constantly discharged out; when the fresh liver changes from blood red to white, liver processing is completed. The toxin-removed animal liver is made into the food, nutrient substances in the liver are supplemented, meanwhile toxic substance ingestion is avoided, and the liver food is safer and healthy. In addition, the animal liver subjected to toxin removal processing has not poor smell and dry and hard taste existing in the common liver. Compared with common liver foods, the product has a more elastic, softer and unique taste and is more delicious.

Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

The invention discloses a formula and production method of a pesticide for preventing and controlling icerya purchasi. The pesticide is prepared by adding tobacco powder and artemisia annua powder to a certain amount of boiled water in a heating container in proportion to be sufficiently stirred and decocted, naturally cooling and filtering the decocted liquid, packaging and sealing clear liquid obtained after filtering, and adding water to dilute the stock solution for spraying when the stock solution is used. The formula and preparation method of the pesticide are unique, the pesticide is a biological pesticide, the prevention and control effect on icerya purchasi damage can reach 93%, and the pest killing effect is remarkable. The pesticide is free of toxin and harm to humans, animals and trees and is safe to use. The production raw materials of the pesticide are wide in source, tobacco powder is screen residue waste in tobacco production, artemisia annua is field weed, waste is used, and cost is low.

The invention relates to construction and expression for campylobacter jejuni cytolethaldistending toxin expression vectors, and preparation for a monoclonal antibody. The campylobacter jejuni cytolethaldistending toxin expression vectors are pET-cdtC and pGEX-6p-1-cdtC. A preparation method for an anti-CdtC monoclonal antibody comprises the following steps of: (1) performing prokaryotic expression and purification on the proteins of his-CdtC and GST-CdtC; (2) immunizing BABL/c mice by GST-CdtC, fusing the splenocytes of the mice with high titers with SP2/0 cells, and culturing and screening cell strains secreting a specific antibody; (3) filling the screened cell strains in the abdominal cavities of the BABL/c mice, so as to obtain high-concentration monoclonal antibody, namely, to finish preparation for the monoclonal antibody; and (4) determining the titers of the supernatant and the ascites of the monoclonal antibody.

The invention discloses a method for measuring surface oil of natural protein fibers and belongs to the field of natural textile fiber production and detection. The method comprises the steps of measuring the surface oil contents of four standard samples, which serve as references, by a conventional extraction method in order to shorten the time of detecting the surface oil content of fibers such as furs and wools and reduce the use of an organic solvent, and obtaining a blank sample with zero oil content; putting the blank sample and a non-extracted standard sample under certain constant temperature, and maintaining the temperature for 30 minutes; measuring in sequence the blank sample and the non-extracted standard sample through a micro nuclear magnetic resonance spectrometer, and constructing a detection equation according to a difference value between nuclear magnetic resonance signals of the standard sample and the blank sample and the corresponding oil content; and measuring the oil rate of a sample to be detected through the detection equation in reference to the blank sample. According to the method, the influence, which is caused by impurities in the sample oil, on a result is alleviated, so that the test time is shortened from 2 working days to 30 minutes; the repetitiveness is high, damage is avoided, and toxins and harms are avoided.

The invention provides a cucumber wilt resistance breeding method. The breeding method comprises the following steps: S1 selecting a breeding main parent and a resistant source parent; S2 performing cross breeding; S3 preparing a crude toxin extracting solution; S4 respectively performing anther induction culture on the cucumber breeding main parent and the resistant source parent; S5 determiningthe crude toxin screening concentration; S6 performing anther culture on a hybrid F1 generation; S7 transplanting flower culture seedlings after rooting; S8 performing wilt resistance identification and offspring screening. Hybrid breeding and a haploid breeding technology are combined to significantly improve the cucumber wilt resistance breeding efficiency and shorten the breeding period, and anewly developed cucumber wilt resistant line is good in resistance and further excellent in agronomic trait; while the yield is ensured, the application amount of pesticides and reduce environmental pollution can be reduced; the method is an effective way to rapidly breed new varieties resistant to cucumber wilt.

The invention belongs to the field of wine brewing, in particular relates to a brewing method, and more particularly relates to a brewing method of agaricus bisporus and chenopodium quinata wine. According to the brewing method, agaricus bisporus and chenopodium quinata are used as raw materials, novel wine is prepared through fermentation, and the efficacies of reducing blood glucose, lowering blood pressure, reducing blood fat, resisting oxidation, resisting cancer, improving body immunity, relieving cough and reducing sputum, inducing diuresis and relaxing the bowels, expelling toxins and losing weight, and the like are enhanced; due to the adoption of the extrusion puffing method for pretreating chenopodium quinate, the utilization rate of chenopodium quinata is increased, and the costis reduced; the agaricus bisporus and chenopodium quinata wine contains rich nutritional ingredients including amino acids, unsaturated fatty acids, vitamins, minerals and polysaccharides, is convenient to use, is pure and soft in taste, is suitable for wide consumers, and has the broad market and development prospects.

The invention discloses a feed additive with coated sodium humate and a preparation method and processing apparatus thereof. The feed additive comprises, by weight, 85-95 parts of sodium humate, 2-8 parts of 52-degree palm oil, and 2-8 parts of montmorillonite. The sodium humate provided herein is gray white uniform granules which can be added to a feed without affecting the appearance of the feed; the montmorillonite covering the outside can adsorb mildews and toxins; the palm oil ensures that the sodium humate gains no contact with gastric juice in the stomach and is decomposed released without affecting the action of the sodium humate; lipids required for the growth of an animal can be provided. In addition, the feed additive made via the preparation method has excellent effect, can increase evidently the body weight, and can reduce the diarrhea rate significantly. The processing apparatus herein has high processing efficiency and brings great conveniences of production to people.

The invention relates to heatstroke prevention health tea. The heatstroke prevention health tea is prepared from, by weight, 8-9 parts of radix ophiopogonis, 6-7 parts of roots of straight ladybell, 3-5 parts of fructus schisandrae chinensis, 15-20 parts of chrysanthemum flowers, 17-19 parts of herba menthae flowers, 3-5 parts of herba dendrobii, 11-13 parts of radix glycyrrhizae, 30-35 parts of green tea, 3-4 parts of ginger, 6-9 parts of plumula nelumbinis, 9-11 parts of herba lophatheri, 7-9 parts of honeysuckle flowers, 10-15 parts of radix puerariae, 3-8 parts of purple perilla, 5-8 parts of hawthorn fruits, 4-6 parts of red dates, 3-7 parts of fructus lycii, 15-20 parts of honey, 11-16 parts of cape jasmine flower, 7-11 parts of lemon, 3-6 parts of corn stigma, 2-4 parts of balsam pears, 3-4 parts of towel gourds, 5-6 parts of watermelon peel, 7-11 parts of green oranges, 13-15 parts of cablin potchouli herb leaves, 1-3 parts of wax gourds, 1-3 parts of semen coicis and 2-4 parts of olive. The heatstroke prevention health tea has the effects of clearing heat, expelling damp, perspiring, dispelling wind, dissipating heat, preventing heatstroke, resolving toxin with clearing coolness, calming the mind, clearing bothers, enriching the blood, cultivating qi, nourishing the liver to improve visual acuity and the like, is abundant in nutrition, has no any toxic or side effects and is suitable for different people to drink in summer.

The invention discloses a microemulsion for prevention constipation of diabetics and a preparation method thereof, and relates to the field of healthcare food. The microemulsion comprises: 20-30% of camellia oil, 30-40% of surfactant, 0.1-3.0% of fructo-oligose, 0.1-1.0% of stachyose, 0.01-0.05% of preservative, and 0.5-1.0% of acidulant, with the balance being glycerin and distilled water. The preparation method comprises steps of dissolving the fructo-oligose, the stachyose, the preservative and the acidulant into the distilled water, then uniformly mixing them with the glycerin, adding Tween-80 to obtain a clear transparent solution; mixing the camellia oil with Span-80 to obtain a yellow clear solution; adding the clear transparent solution into the yellow clear solution to obtain a clear orange-yellow microemulsion. Comparing to prior arts, the microemulsion for preventing constipation of diabetics does not contain sugar, so it is not only of a good effect, but also is free of toxin and side effects. With a good taste and an attractive appearance, the microemulsion for preventing constipation of diabetics is an ideal functional food for diabetics to coordinate intestines and stomach as well as prevent constipation.

The invention discloses a traditional Chinese medicine preparation having weight-losing toxin-expelling efficacies; the traditional Chinese medicine preparation is prepared by adding water into the following raw materials in parts by weight and decocting: 15-25 parts of poria cocos, 5-15 parts of cassia twig, 5-15 parts of honey-fried liquorice, 5-15 parts of red ginseng, 25-35 parts of raw ginger, 5-15 parts of rhizoma atractylodis macrocephalae, 5-15 parts of Chinese ephedra, 10-20 parts of ossa draconis, and 10-20 parts of oyster. According to the drug, 9 kinds of traditional Chinese medicines are selected for scientific compatibility, the formula is simple, the pharmaceutical raw materials cooperatively act for achieving the effects of replenishing blood, tonifying qi, expelling toxin and lowering lipid, and the preparation is quick to become effective, treats both symptoms and root causes, and has no toxic or side effect; and moreover, the traditional Chinese medicine preparation is simple in preparing method, convenient to take and low in cost, and has the effective rate reaching more than or equal to 97%.

Organic compounds showing the ability to inhibit effector toxin secretion or translocation mediated by bacterial type III secretion systems are disclosed. The disclosed type III secretion system inhibitor compounds are useful for combating infections by Gram-negative bacteria such as Salmonella spp., Shigella flexneri, Psendomonas spp., Yersinia spp., en tero pathogenic and enteroinvasive Escherichia coli, and Chlamydia spp. having such type III secretion systems.

The invention discloses a wild ganoderma product for resisting cancer and tumor and removing liver toxin and a preparation technology. According to the invention, wild ganoderma lucidum is adopted asraw material, and the wild ganoderma product for resisting cancer and tumor and removing liver toxin is finally prepared by the steps of washing, sterilizing, drying and dicing and the like. The product has the advantages of inhibiting cancer cell growth, attending acute and chronic hepatitis, early cirrhosis, esophageal cancer, lung cancer, liver cancer, nasopharyngeal carcinoma, pancreatic cancer, neurasthenia, intestinal diarrhea, rheumatoid tuberculosis,diabetes, gastrointestinal diseases, nourishing physical, lowering blood sugar, moistening the lungs and kidneys, maintaining beauty and keeping young, enhancing immunity, and enriches in ergosterol, ganoderic acid, ganoderma lucidum polysaccharides, amino acids, polypeptides, adenosine, triterpenoids and various trace elements, whereinthe organic germanium content is as high as 12380 ppm and the organic selenium content is also high. The product can be made into five-color ganoderma lucidum tea, ganoderma lucidum beverage, ganoderma lucidum oral liquid, five-color ganoderma lucidum wine, and other homologous products of medicines and foods. The product provided by the invention can also be made into traditional Chinese medicine prescriptions such as paste, pill and pellet.

The invention relates to a ground face washing soap with traditional Chinese medicines and effects of removing acne and diminishing inflammation. The ground face washing soap mainly comprises a mature cold soap and traditional Chinese medicine liquid decoction of the mature cold soap. The mature cold soap comprises, by weight, 75 parts of coconut oil, 75 parts of palm oil, 350 parts of camellia oil, 66 parts of sodium hydroxide, 365 parts of traditional Chinese medicine liquid and 15 parts of honey, and the traditional Chinese medicine liquid comprises, by weight, 10 parts of fresh herba portulacae, 10 parts of herba taraxaci, 10 parts of flos chrysanthemi, 10 parts of nidus vespae, 15 parts of nervilia fordii and 20 parts of Diqingpi. The ground face washing soap has the advantages that the traditional Chinese medicine liquid with the effects of removing the acne and diminishing inflammation is not heated in integral procedures and cannot be easily destroyed by strong alkali, and accordingly functions of the traditional Chinese medicine liquid can be reserved; the ground face washing soap is manually sufficiently ground, accordingly, the finished soap is fine and smooth and is soft in wash feel, effects of tightening pores, replenishing water, preserving moisture, expelling toxin, controlling oil and removing acne marks can be realized while skins are cleaned, and the ground face washing soap has functions of removing the acne, diminishing inflammation and moistening the skins and is suitable for the skins with the acne.

The invention relates to a toxin-producing culture medium of AFG1 and a toxin-producing fermentation method of the AFG1 of aspergillus parasiticus. The culture medium contains 1-3% of a yeast extract; and the fermentation medium is an enrichment culture medium and a solid fermentation medium. According to the toxin-producing culture medium of the AFG1 provided by the invention, the problems that existing AFG1 in grains naturally infected by mycetes is low in concentration, uneven, long in elapsed time and the like are solved.

The invention discloses a chemical treatment method for cottonseed protein detoxification processing, comprising the following steps: adding a hydrogen peroxide solution to cottonseed meal powder forfirst immersion, and collecting a solid matter after solid-liquid separation; performing washing and drying treatment on the solid matter to obtain detoxified cottonseed meal. The technical scheme provided by the invention is to utilize the oxidizing property of hydrogen peroxide to oxidize the aromatic aldehyde group of free gossypol in cottonseed meal into a carboxyl group, thereby completely removing cotton toxin in the cottonseed meal, and the residual hydrogen peroxide in the cottonseed meal is easy to separate and remove after being washed with clear water, so that the cottonseed meal subjected to detoxification treatment is free of solvent residue, and further has the advantages of high protein recovery rate and high dephenolization rate, and the process is simple and easy to operate, and can meet the industrialization scale production needs.

The invention discloses a detoxifying formula prepared by using plant animal essence and a preparation process thereof. The formula comprises: field thistle herb, Chinese yams, purslane, heep seeds, gingko, cassia seeds, poria cocos, gordon euryale seeds, inner membrane of chicken gizzard, Chinese dates, Chinese wolfberry fruits, longan aril, cinnamon, rice beans, dried ginger, lily bulbs, cape jasmine fruits, sharp-leaf glangal fruits, red-skin lotus seeds, solomonseal rhizomes, chrysanthemums, black-tail snakes, radish seeds, black sesame, black beans, black fungus, kudzuvine roots and kelp.The preparation process includes the following steps: step 1, raw material selection; step 2, raw material quantification; step 3, raw material cleaning; step 4, raw material drying; step 5, raw material crushing; step 6, raw material mixing; and step 7, inspection of packaging. The invention builds a complete novel food production chain, adopts high-tech quantitative measures to remove toxin residues in all foods, achieves food safety and reliability after production, prevents people from getting sick after being eaten, and respectively prepares different foods to achieve the purposes of conditioning different diseases of human beings and achieving rehabilitation.