Cucumber wilt resistance breeding method

[0032] From 2011 to 2014, the cucumber wilt resistance breeding was carried out according to the technical scheme of the present invention, which specifically included the following steps:

[0033] S1 Selection of main parent and resistant parent for breeding: In 2011, the cucumber variety 'Jufeng No.8' mainly cultivated in Shouguang City, Shandong Province was selected as the main parent for breeding. 'Jufeng No.8' has strong growth potential and medium-sized leaves. Mainly vine fruiting melons, fruiting melons are continuous, the melons are straight and stick-shaped, the color of the melons is dark green and shiny, the flesh is thick and green, the handles are short, the white thorns, the thorns are moderate, no edges, the melons are about 40cm long, single The melon weighs about 200 grams, and the yield per mu is about 20,000 kilograms, but it is susceptible to Fusarium wilt resistance. Cucumber variety 'Jinlian No. 1' was selected as the parent of fusarium wilt resistance. Th

[0043] Test the influence of the induction medium in the step S2 of the present invention on the induction effect of the anther callus of the two parents of cucumber: only the induction medium in the step S2 is replaced by B5+0.25mg / L6-BA+1.0mg / L2, 4- D+1.0mg / L AgNO 3 (From the literature "Research on Induction and Regeneration of Callus in Cucumber Anther Culture"), the callus induction rate was counted, and the results are shown in Table 1 below.

[0044]

[0045]As can be seen from the results in Table 1, compared with Example 2, using the induction medium of the present invention to cultivate the anthers of the two parents of 'Jufeng No. 8' and 'Jinlian No. 1' can obtain higher induction rate; at first it may be that there is a certain difference in the callus induction rate between different genotypes of cucumbers, and secondly, the induction medium of the present invention has added aminonew fat, lanthanum nitrate, diethyl sulfate, cysteine ​​hydrochloride, Betain

[0047] Test the influence of the differentiation medium of the present invention on the callus differentiation effect of the two parents of cucumber in the case of adding crude toxin: only replace the differentiation medium in step S4 with B5+0.5mg / LNAA+1.5mg / LKT+ 1.0 mg / L AgNO 3 (From the literature "Studies on Induction and Regeneration of Cucumber Anther Culture Callus"), and adding the crude toxin extract with the same concentration gradient, the callus differentiation rate was counted, the statistical results were summarized with Example 1, and integrated into the following table 2 .

[0048]

[0049] As can be seen from the results in Table 2, when the anther callus differentiation rate of 'Jufeng No. 8' in Example 1 was 1.5%-2.5%, the crude toxin concentration added to its differentiation medium was about 5%, and the concentration of 'Jinlian No. 1 When the anther callus differentiation rate of ' is at 1.5%-2.5%, the crude toxin concentration added by its differe