Method for cultivating immature capsicum seeds and rapidly propagating seedlings

[0029] Example 1:

[0030] The invention provides a method for cultivating immature seeds of pepper and rapid propagation of seedlings, which comprises the following steps:

[0031] (A) Obtaining immature seeds: Take the pepper 6421 green fruit 25-30 days after pollination in the field, put it in a ziplock bag, seal the bag tightly, leave it at room temperature for 3 days, take it out, rinse it under running water, and use 75% alcohol Wipe the peel 2-3 times with the cotton ball. Then use a scalpel to cut the fruit in half lengthwise, and finally use tweezers to gently remove the immature seeds for inoculation.

[0032] (B) Immature seed germination culture: Immature seeds are inoculated in a seed germination medium on a clean bench and cultivated until seedlings are grown. The seed germination medium formula is 1 / 2MS medium (see Table 1) + 0.5 mg / L gibberellin + 20 g / L sucrose + 6 g / L agar powder, and the pH value is 5.8. The culture conditions are as follows: the culture te

[0044] Example 2:

[0045] Inoculate the immature seeds in the seed germination medium on the ultra-clean workbench and cultivate until the seedlings grow. The medium formula is 0.7g / L ammonium nitrate+1.5g / L potassium nitrate+0.07g / L potassium dihydrogen phosphate+0.5mg / L gibberellin+20g / L sucrose+6g / L agar Powder, other operations and conditions are the same as in Example 1. Observation after 7 days of inoculation, it was found that the germination rate of seeds in Example 2 and Example 1 were both greater than 95%, indicating that it is feasible to replace 1 / 2MS medium with inorganic salts to cultivate seeds. This method saves experimental costs and reduces experiments. step.

[0046] Example 3:

[0047] The immature seeds of pepper were inoculated into a medium supplemented with 0.5 g / L of streptomycin, and the experiment was not carried out on an ultra-clean workbench. Other operations and conditions were the same as in Example 2. Observation after 3 days of inoculation found that adding low concentration of streptomycin to the culture medium has the same effect as inoculating in a clean bench. The infection rate of seeds in Example 3 and Example 2 is less than 1%; 7 days after inoculation, observe the seed germination rate It was found that the addition of streptomycin did not affect the germination of seeds. The seed germination rates in Example 3 and Example 2 were both greater than 95%, indicating that the method of adding streptomycin can simplify the inoculation process.