42 results about "Germination" patented technology

The present invention relates to the abacterial seeding and tissue culture of Paphiopedilum. The Dendrobium is one kind of national first order protected plant and is hard to germinate naturally owing to its incomplete development of embryo. The present invention provides technology of abacterial seeding and tissue culture of Paphiopedilum to obtain great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding, proliferate small seedlings and obtain complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

The invention provides a high and steep slope vegetation recovery structure. The surface layer of a high and steep slope body is covered with a growth substrate; a reinforced mesh is positioned in the growth substrate; water-blocking grooves are horizontally distributed in the growth substrate; a seed germination substrate is longitudinally embedded in a strip shape into the growth substrate; seed germination holes are distributed in microporous thin films according to the position of the seed germination substrate; the surfaces of the growth substrate and the seed germination substrate are covered with the microporous thin films in a fitted manner; the microporous thin films are clung to the bottoms of the water-blocking grooves at the water-blocking grooves. The slope substrate of the recovery structure is stable; soil or broken stones on the slope surface are effectively prevented from sliding off; the recovery structure is light in self weight, thereby facilitating the fixation of a greening substrate, increasing the germination rate of seeds and increasing the cover degree of vegetation; the water-blocking grooves are capable of prolonging the retaining time of the moisture from natural rainfall or manual watering on the microporous thin films to further enable the moisture to penetrate the growth substrate, thereby avoiding the runoff loss under the high and steep slope condition and the erosion of the runoff on the growth substrate or the seed germination substrate.

The invention relates to new application of melatonin to improving saline-alkaline stress resistance of plant seeds. A product taking the melatonin as an effective ingredient is applied to the plant seeds under saline-alkaline stress, and the melatonin is a compound with the high lipophicity and hydrophilcity and can get in and out of plant cells freely, so that the melatonin can play a role in plant tissues. The melatonin can be applied to the plant seeds under the saline-alkaline stress conveniently, can be used for promoting the germination rate and the germination potential and reducing the salt damage rate of the plant seeds under the saline-alkaline stress, and can be used for promoting the seed germination and seed vigor, so that the purposes of keeping a full stand of seedlings, strengthening the seedlings, and preventing the reduction of yield and quality, caused by the saline-alkaline stress, can be achieved.

The invention discloses a pollution-free paddy rice planting method comprising following steps: seed pretreatment: paddy rice seeds are successively subjected to soaking in a soak solution, voltage application in a corona field and mixing to obtain paddy rice seeds for sowing; 2. land preparation: pig dung layer, three different bio-fertilizers and marsh manure layer are successively laminated and embedded in multiple trenches of soil and fermentation on-site is performed to obtain fertile seedling growing bed; 3. seedling growing and self-made nutrient soil: seedling growing holes of a seedling growing tray is filled with the nutrient soil, the paddy rice seeds for sowing are spread in the seedling growing holes, watering and fertilization management are performed to obtain seedlings to be transplanted; 4. transplanting. According to the method of the invention, through pretreatment of paddy rice seeds and land preparation of rice seedling beds, fertile seedling growing beds which can prevent insect disease are obtained to increase the germination rate and seedling emergency rate of paddy rice, improve the quality of rice seedlings and make the seedlings robust and root well developed; the seedlings are provided with strong insect disease-resistant ability; high quality and pollution-free paddy rice is obtained.

The invention discloses a rapid cultivation method for Chinese rose landscape modelling. The method includes the steps of planting grafted seedlings or cutting seedlings in a cultivation container, and managing water and fertilizers in an initial planting stage; binding and sizing a modelling support on the top of the cultivation container after rust prevention of the modelling support; binding the main branches of limbing roses from bottom to top along the base of the modelling support, and according to 30-70 degrees of a lateral angle on the main branches, binding the main branches at the outer side of the modelling support; binding first-grade branches uniformly on the modelling support by a lateral angle of 30-70 degrees, and binding second-grade branches in the same 30-70 degree lateral mode; when the main braches, the first-grade branches and the second-grade branches of the limbing roses grow and are bound until the modelling support cannot be seen, obtaining the modelled Chinese rose landscape modelling. The method can promote bud germination, growth and flowering, not only the leaf coverage rate is high, the landscape modelling is complete, the seedling-planted rose modelling culture period is short, and the modelling can be formed in the current year, but also the formed modelling can be moved.

The invention discloses an in-vitro rapid propagation method of rare and endangered plant ammopiptanthus mongolicus, belongs to the biological field and aims to solve the problems of severe browning and differentiation difficulty in callus induction in existing methods. The in-vitro rapid propagation method of the ammopiptanthus mongolicus comprises steps of obtaining of ammopiptanthus mongolicus explants, induction culture of cluster buds, extension culture of the cluster buds, rooting culture and expansion propagation. Compared with existing in-vitro culture methods of the ammopiptanthus mongolicus, the method has the advantages that cotyledonary nodes are directly subjected to induction of cluster buds and rooting is performed, callus cannot be produced in the process, thus, the step of callus induction is omitted, the cotyledonary nodes are directly cultured into adventitious buds, the in-vitro culture time is greatly shortened, and the whole process from seed germination, obtaining of cotyledonary nodes, induction and extension of the cluster buds to rooting only takes 79 d.

The invention discloses a method for planting Taxus baccata in Zhejiang, China. The method comprises a Taxus baccata seed temperature-varying germination accelerating treatment method, a Taxus baccata seed seedbed seedling raising method and a Taxus baccata seedling transplanting, field planting and field management method. The transplantation survival rate in the planting method reaches more than 90 percent; by taking branch leaves of 2-3 year-old Taxus baccata and detecting the content of 10-deacetyl baccatin III through high-performance liquid detection, the content of 10-deacetyl baccatin III in trunk branch leaves reaches 0.20-0.30 percent and is higher than content (which is 0.10-0.20 percent) of 10-deacetyl baccatin III in the trunk branch leaves of Taxus baccata in the place of origin (United Kingdom), the problem that the Taxus baccata is difficult to germinate, grow and survive especially in China is effectively solved, the problem of artificial planting of Taxus baccata medicinal seedlings in Zhejiang, China, the effect of introducing excellent Taxus tree resources is achieved and the problem of raw material sources of semi-synthetic taxane anti-cancer raw material medicine in China is solved.

The invention discloses an eggplant planting method. The eggplant planting method comprises the following steps: when immersing seeds with warm water, continuously scrubbing and washing the seeds to remove mucus on planting surfaces, so as to accelerate water absorption and breathing and promote germination; in a germination promoting process, alternately carrying out change-temperature treatment every day so that sprouts are regular, thick and sound; at a fruiting later period of eggplants, applying nitrogen fertilizer, phosphorus fertilizer, calcium fertilizer and potassium fertilizer to the eggplants, wherein the ratio of the nitrogen fertilizer to the phosphorus fertilizer to the calcium fertilizer to the potassium fertilizer is 3 to 1 to 4 to 5. At the fruiting later period of the eggplants, the absorbing capacity on the fertilizers is remarkably improved, and long eggplant fruits are separated from leaves or leaf stalks, so as to prevent a bent fruit phenomenon; 1 percent flower dipping medicine is smeared at the inner side of the bent fruit, so as to correct fruit bending; the eggplants planted by adopting the planting method have the advantages of high germination rate, high yield and good shape, has a huge market potential and has a wide prospect.

The invention provides an indoor artificial photohydroponic method for dianthus chinensis l flower plant. The method comprises the following steps: in seeding germination acceleration period, early seedling cultivation period, later seedling cultivation period and cultivation period, seeding and germination acceleration treatment on dianthus chinensis l. seeds are performed, different illuminationconditions are adopted in the early seedling cultivation period, the later seedling cultivation period and the cultivation period and cooperate with different nutrient solutions for cultivation, thespecific conductance and pH of a nutrient solution and the temperature and humidity environment of all periods are controlled, the corresponding light intensity and illumination time are set accordingto the growth habits of dianthus chinensis l. in different periods, and illumination is alternately carried out in all the periods. The complicated operation of outdoor planting of the dianthus chinensis l. is simplified, the cleanliness of dianthus chinensis l. flowers is ensured, the growth period of the dianthus chinensis l. is shortened, growth is more robust, and the output per unit area isgreatly increased.

The invention discloses a method for separating and purifying 4,8-DHT from pecan exocarp and an application of 4,8-DHT. The method comprises the following steps: performing ultrasonic extraction to obtain a pecan exocarp extracting liquid by virtue of independent excitation type ultrasonic sounding, performing reduced pressure concentration, then passing through X-5 macroporous resin, performing gradient elution by using a mixed solution of water and ethanol, collecting an eluate obtained by eluting with a mixed liquid of water and ethanol in a volume ratio of 4 to 6, performing reduced pressure concentration to obtain a concentrated solution, performing silica-gel column chromatography, performing gradient elution by using a mixed solvent of petroleum ether and ethyl acetate, totally collecting 36 bottles of flow components by collecting a bottle of the flow components per 1/2 column volume, combining the tenth to eighteenth bottles of the flow components, performing reduced pressure concentration, and performing ethyl acetate crystallization to obtain a 4,8-dyhydroxy-1-tetralone crystalline compound. 4,8-DHT disclosed by the invention is of a 1:1 raceme, is less in impurity, has the purity of 99.5-99.8%, and ensures that the extraction rate reaches more than 94%; and the 4,8-DHT raceme has the effects on inhibiting seed germination and seedling growth of plants.

The invention provides a vegetable seed sterilization activation treatment agent, preparation and application thereof. The treatment agent includes a treatment agent A and a treatment agent B, whereinthe treatment agent A comprises the following percentage components: 5-20% of apple cider vinegar, 5-15% of duchesnea indica juice, 2-4% of apigenin, 3-5% of konjac gum and the balance distilled water; and the treatment agent B comprises 10-20% of a plant extract, 2-8% of diethyl aminoethyl hexanoate, 0.3-1.5% of bacillus thuringiensis, 2-5% of a bactericide, and the balance distilled water. According to the invention, through reasonable raw material proportion selection and pretreatment of vegetable seeds before planting, the seed activity and nutrient intake ability are effectively improved, the seeds can be healthy and full, the germination rate is up to 99.7%, also the pest and disease damage rate tends to zero, the overall growth vigor is good, and the yield and quality are obviouslyimproved.

The invention discloses a method for artificial cultivation of Leontopodium smithianum, which mainly includes the steps of seed preparation, germination bed treatment, seeding, land selection and preparation, transplanting and post-transplant management. By the method, large-scale artificial cultivation of Leontopodium smithianum can be realized. The method is easy to master, low in cost, easy toimplement and effective in implementation, and accordingly a solid base for garden promotion is laid.

The invention discloses a plant growth adjusting lamp, which is characterized in that: a mixed spectrum of the lamp comprises a spectral wavelength of 440+/-20nm and a light energy peak of 93+/-7 percent, a spectral wavelength of 550+/-20nm and a light energy peak of 90+/-5 percent, a spectral wavelength of 660+/-30nm and a light energy peak of 95+/-5 percent and a spectral wavelength of 415+/-5nm and a light energy peak of 50+/-15 percent. The lamp is a fluorescent lamp, a high-frequency/low-frequency electroless lamp or a light-emitting diode (LED) lamp. The lamp can greatly shorten plant seed germination time and mature time, and is low in energy consumption and low in cost.

The invention discloses a biological reader capable of rapidly verifying the sterilizing effect. The biological reader mainly comprises an excitation light source, a fluorescent light filtering disc system, an optical signal acquisition system, a fluorescent biological indicator, a temperature control system, an image processing system and an output result; the main principle and the working process of the biological reader are as follows: a biological indicator after sterilization is inserted into the biological reader, spores germinate in a culture solution at appropriate temperature under the action of a germination accelerator, an active enzyme is generated and reacts with an added fluorescent material in a culture medium, the excitation light source emits excitation light with the specific wavelength and irradiates the fluorescent biological indicator, the fluorescent material in the fluorescent biological indicator can emit fluorescent light with the specific wavelength after excitation, the fluorescent light is captured and imaged by a high-sensitivity digitized CCD, and an image processing system can process, compare and analyze acquired image information and judge whether the sterilization is effective within a short time.

The invention discloses a high-flux seed germinator, which relates to the technical fields of agriculture and garden seedling raising and is used for solving the problems of difficulty in water supplementation and control, complex procedures and low seedling raising efficiency in the breeding process in the prior art. The high-flux seed germinator is as follows: a water retention tray is provided with a first number; a germination pot rack is movably arranged in the water retention tray; the germination pot rack is provided with M rows and N columns of first holes; a germination pot is inserted into each first hole; the external diameter of the top of each germination pot is greater than the internal diameter of the corresponding first hole; the external diameter of the bottom of each germination pot is less than the internal diameter of the corresponding first hole; the bottom of each germination pot is provided with a second hole; a germination pot cover is arranged on a top opening of the germination pot; each germination pot is provided with a second number; water-absorbing sponge and filter paper are arranged inside the germination pot; and the filter paper is arranged on the top of the water-absorbing sponge.

The invention provides a method for live broadcast of seedling raising by using nutritional seed bags. Firstly, nutrient soil cakes are put in net bags to form the nutritional seed bags; a seed slot is formed in the top of the nutritional seed bag; 1-3 seeds are put in the seed slot, forest land is prepared as required, and planting holes are dug according to planting requirements; the nutritional seed bags are put in the planting holes; the surfaces of the nutritional seeds are coated with coal ash residues or ash soil, wherein the soil covering thickness is 1-2 cm. According to the scheme, nutritional ingredients are contained in the bags, seed germination and rooting are not limited, rapid and healthy growth can be achieved, and a pesticide in the bags can prevent seeds and samplings from being wormy.

The invention relates to a bergapten endophyte separating and cultivation method and application thereof. Bergapten endophyte is obtained from bergapten stems, bergapten leaves, bergapten roots and abergapten stem, leaf and root mixture, experiment results show that the rooting rate of bergapten cuttage can be increased obviously by the bergapten endophyte, particularly bergapten root endophyte,the rooting time of the bergapten cuttage is shortened, the germination rate of bergapten seeds can be increased remarkably, the time required for germination of the bergapten seeds is shortened, therefore, bergapten seed propagation can be widely applied to artificial cultivation of the bergapten favorably, and rapid development of the bergapten industry is promoted.

The invention belongs to the technical field of seedling cultivation, and particularly relates to a cutting seedling culture method capable of promoting rooting and germination of ascendens mucronatum. The method includes that semi-lignified branches on ascendens mucronatum are used as scion, extract of ascendns mucronatum leaves is used to cooperate with indoleacetic acid or naphthylacetic acid to accelerating rooting of the scion, the scion is inserted into a culture medium, the culture medium is thoroughly irrigated by spraying water and then compacted, and quick germination and rooting ofcultivated ascendens mucronatum can be realized by continuously controlling temperature and relative humidity of the culture medium after the scion is inserted for 30-45 days.

The invention discloses a milk vetch seed germination box which comprises a cultivating groove and a germinating bed arranged in the cultivating groove. The germinating bed further comprises a water storage sponge layer, a wet cloth layer and a seed cultivating layer. The cultivating groove is arranged in a cultivating box larger than the volume of the cultivating groove, and a heat preservation layer is arranged in an interlayer of the cultivating box. The cultivating box is provided with a cover body. The milk vetch seed germination box has the advantages of being simple in structure, good in heat preservation effect and wide in application range and is also applicable to germination tests of small seeds such as rape and tobaccos.

The invention discloses a germination accelerating device for wuta-tsai breeding and a using method. The germination accelerating device comprises a base, a liquid storage barrel, a threaded sleeve and an outer disc, the liquid storage barrel and the threaded sleeve are fixed on the upper surface of the base side by side, a U-shaped hole is formed in the base, and one end of the U-shaped hole is communicated with the liquid storage barrel while the other end of the liquid storage barrel is communicated with the threaded sleeve. After a seed storage barrel is mounted, a proper amount of seeds are poured in, germination accelerating liquid enters the liquid storage barrel, a rubber plug of a liquid adding port is covered, and a motor is started to drive a main shaft to rotate to drive an inner disc and an outer disc to downwards move to push the germination accelerating liquid into the liquid storage barrel; after catalysis is finished by completely soaking the seeds, the rubber plug ofthe liquid adding port is pulled away, the motor reversely rotates to drive the inner disc and the main shaft to ascend, inside gas is extracted and discharged from a seed inlet, liquid in the liquidstorage barrel is withdrawn and slowly flows out from the liquid adding port to complete liquid discharging, and air pressure change is utilized to complete quick adding and extracting of catalytic liquid.

The invention discloses a seedling-raising substrate and a preparation method thereof, and relates to the field of plant seedling raising. The seedling-raising substrate is prepared from, by mass, sandy soil, magnesium sulfate, rotten cow dung, a water-absorbing water-retaining agent, carburizing rice husk, trachycarpus fortunei bark fibers, calcium magnesium phosphate fertilizer, nitrogen-phosphorus-potassium compound fertilizer, ammonium sulfate, monoammonium phosphate, boric acid, polyaspartic acid, glucosamine oligosaccharides, a plant disease prevention agent and activation resistant bacteria. According to the technical scheme, the substrate has the functions of sufficient fertilizer efficiency, nutrition slow release, disease resistance and stress tolerance, comprehensive nutrition, high pertinence and the like, and various indexes reach the industrial standard. By means of batched production and application in multiple seedling-raising factories, the seedling-raising substrate can increase the germination rate of seeds and seedling emergence rate, seedlings are regular, robust growth is achieved, the seedlings are healthy and strong, rampant growth, root rot, dwarfing and rigidifying do not exist, flower bud differentiation of the seedlings is normal, and the property, yield and quality of the crop variety are guaranteed.

The invention relates to a method for cultivating transgenic melanin strawberries, which belongs to the field of planting technology. The method uses black soybean pigment gene, culture medium, sodium thiosulfate, magnesium sulfate, kudzu root dregs, starch, sodium sulfate, hypostone, and water as Raw materials, through gene transfer technology, the black soybean pigment gene extracted from black soybeans is injected into the embryos of strawberry seeds, and a compound organic nutrient solution containing potassium, iron and other trace elements is added to the soil to cultivate a vitamin-rich E. Black strawberries with iron elements. The appearance of the strawberry is black, the particles are large and uniform in size; rich in vitamin E and iron, which are easily absorbed by the human body, have a high biotransformation rate, and have special curative effects on patients with cerebral thrombosis; It has strong resistance to adversity and can be stored for 25 to 30 days at a low temperature of -5 to -10°C.

The Eurya japonica winter seeding method for culturing seedling includes collecting berry in the time from August to November, pounding, washing to eliminate pulp liquid, filtering to obtain seed, seeding to wind sheltering and sunny seedling bed timely, maintaining temperature and humidity for germination to obtain young seedling, transplanting the young seedling to other wind sheltering and sunny seedling bed in the next spring, maintaining temperature and shading for further growth of the seedling. The present invention obtains Eurya japonica seedling for afforestation by means of sexual propagation.