83 results about "Amino acid" patented technology

The present invention relates to molecules, particularly polypeptides, more particularly immunoglobulins (e.g., antibodies), comprising a variant Fc region, wherein said variant Fc region comprises at least one amino acid modification relative to a wild-type Fc region, which variant Fc region binds FcγRIIIA and/or FcγRIIA with a greater affinity, relative to a comparable molecule comprising the wild-type Fc region. The molecules of the invention are particularly useful in preventing, treating, or ameliorating one or more symptoms associated with a disease, disorder, or infection. The molecules of the invention are particularly useful for the treatment or prevention of a disease or disorder where an enhanced efficacy of effector cell function (e.g., ADCC) mediated by FcγR is desired, e.g., cancer, infectious disease, and in enhancing the therapeutic efficacy of therapeutic antibodies the effect of which is mediated by ADCC.

The invention discloses a plant antifreezer and its preparation method. The plant antifreezer is preparegggggd from the following components of: by weight, 0.5-0.97% of glucose, 0.5-0.97% of cane sugar, 0.2-0.5% of potassium dihydrogen phosphate, 0.18-0.21% of novel monomolecular lipid film, 0.06-0.17% of paclobutrazol, 0.2-0.33% of amino acid, 0.02-0.05% of 6-benzylamino-purine, 0.01-0.05% of rapin, 46-48.4% of glycerin and 48.6-51% of water. The plant antifreezer provided by the invention is nontoxic and harmless, doesn't pollute the environment, and can be directly sprinkled on plant leaf surfaces, flowers and flower bus. The preparation method is simple and requires low cost. Effective antifreezing period can reach 15-20 days. By the adoption of the plant antifreezer, lower temperature freeze injury resistance of plants can be enhanced and the freeze injury can be rapidly mitigated after freeze injury. The plant antifreezer provided by the invention can be used to form a protection film on the surface of a plant and protect the plant from cold, and has an effect of preventing diseases.

The invention belongs to the technical field of adsorbing agents and relates to a method for preparing a carbon adsorbing material used for efficiently removing harmful ions from waste water. The method comprises the following step: on the basis of taking the soluble saccharide or furfural as a carbon source and the amino acid or compound containing sulfydryl, carboxyl or amino group as an additive, performing the hydro-thermal reaction on the carbon source and the additive at 160-220 DEG C, thereby generating the carbon solid adsorbing material. The adsorbing material can be used for processing heavy metal ions or transition metal ions in industrial effluent and domestic sewage and the processing effect is excellent. The method has the advantages that the soluble saccharide or furfural is used as a raw material for preparing the carbon solid adsorbing material, the cost of the raw material is low, the preparation process is simple, the solid adsorbing material is synthesized through one step of hydro-thermal reaction, the reaction condition is mild and controllable, and the solid adsorbing material prepared by using the method has excellent stability and contains a large amount of surface groups. The content of the surface groups can be controlled within a large scope without changing the reaction condition. The surface groups have strong interaction with the metal ions, such as sulfydryl and mercury ions, so that the carbon adsorbing material can be used for efficiently removing the harmful ions.

The invention discloses a synthesizing method of sermaglutide. The synthesizing method comprises the following steps of step 1, preparing Fmoc-Gly-Wang resin; step 2, using the Fmoc-Gly-Wang resin, protective amino acids, and octadecandioic acid (OtBu)-gamma-Glu-OtBu-PEG-PEG-OH as the raw materials to prepare full-protective peptide resin which contains Depsipeptide Units; step 3, performing TFA (trifluoroacetic acid) cracking on the peptide resin, so as to obtain crude peptide; step 4, dissolving the crude peptide obtained in step 3, adjusting the pH (potential of hydrogen) value, and converting ester bonds into amide bonds to react, so as to obtain a crude product of the sermaglutide. The synthesizing method has the advantages that by introducing the Depsipeptide Units, the problem of difficulty in sequential synthesizing of the sermaglutide is solved, and the target product is obtained by the reaction of converting the ester bonds into the amide bonds; the purity and yield of the crude peptide are improved, and the synthesizing method is suitable for downstream purifying, and is suitable for industrialized production.

The invention belongs to the field of analytical chemistry and particularly relates to a detecting method for determining various neurotransmitters on the basis of in situ derivation. The method is characterized in that 4'-carbonyl chloride-rhodamine is used as the derivative reagent for the first time, the ultrasonic assistance-in situ derivation-dispersive liquid-liquid microextraction technology is used to derive amino acid and monoamine neurotransmitters, and the ultra-high performance liquid chromatography triple quadrupoles tandem mass spectrum with multiple reaction monitoring modes is used to perform qualitative and quantitative detection; three internal standard substances (isoprenaline, 5-ketoindole-2-carboxylic acid and norleucine) are used for building the linear equations of the neurotransmitters of three chemical structures; the existence of the neurotransmitters is accurately determined by quantification through an internal standard method. The detecting method is simple, fast, high in sensitivity, good in selectivity, good in recovery rate, capable of fast and accurately determining the neurotransmitters, and the like.

The invention relates to anti-tumor activity polypeptide and application thereof.The polypeptide has anti-tumor activity due to the fact that the polypeptide has one of the following amino acid sequences of RMKKRYPTTFVMVV and IGLKRTPMGIV, wherein Y or the second T counting from left to right in RMKKRYPTTFVMVV is modified through phosphorylation, and T in IGLKRTPMGIV is modified through phosphorylation.The polypeptide can be used for preparing an anti-tumor drug.The sequence of the polypeptide is short, and large-scale production is easily achieved; remarkable anti-tumor activity is displayed at a low dosage.The polypeptide has no toxicity to normal tissue cells and has wide application prospects.

The invention discloses a high-protein meal replacement food and a processing method thereof, wherein the meal replacement food is made of the following raw materials by mass ratio: 30 to 42 parts ofprotein powder, 4 to 12 parts of plant source-type powder, 0.1 to 1 part of a taste-improving powder raw material, 0 to 3 parts of other powder raw materials, 20 to 35 parts of a sugar material, 5 to15 parts of an oil material, 2.5 to 5.0 parts of glycerin, 0.1 to 1 part of salt, 0. 3 to 3 parts of drinking water, 0 to 10 parts of other non-greasy liquid or paste materials, 5 to 15 parts of solidparticles, and 0 to 0.5 parts of liquid food essence. The method controls the powder/syrup ratio, and controls the quality problem of the product from the source of the technology; the meal replacement prepared according to the ratio and the process is easier to mold, and the mouthfeel is more comfortable. Different protein sources supplement the needs of various protein/amino acids for the consumer; high-quality dietary fiber ingredients such as chia seeds can be added to increase satiety after eating; and the application scope is wide.

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection. The kit comprises a Seneca valley virus nonstructural protein 3ABC antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, a sample diluent, a chemiluminescence substrate solution, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus nonstructural protein 3ABC is shown as SEQ ID NO.2. The kit provided by the invention uses nonstructural protein 3ABC antigen expressed by a prokaryotic expression system for coating a reaction plate; the antigen consumption is low; whether the Seneca valley virus exists in the pig serum or not can be efficiently detected; in addition, reaction with the swine vesicular disease viruses, hog cholera viruses and porcine circovirus type 2 cannot occur. An enzymatic chemiluminescence reactionsystem is used for judging the result; the detection sensitivity is improved. The kit provided by the invention has the advantages that the specificity is high; sensitivity and high efficiency are realized; good market prospects are realized.

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

The invention relates to the technical field of virus detection, and particularly discloses a novel coronavirus 2019-nCoV double-target antibody detection microsphere complex combination, a preparation method, a kit and a using method of the kit. A microsphere complex combination coupled with an antigen is used; the coupling antigen comprises an S protein of the novel coronavirus 2019-nCoV and anN protein of the novel coronavirus 2019-nCoV, the amino acid sequence of the S protein is as shown in SEQ NO: 1, and the amino acid sequence of the N protein is as shown in SEQ NO: 2. The prepared microsphere complex combination can perform double-target detection on an S antibody and an N antibody, and fully reflects the effect that two main antigens of the novel coronavirus (2019-nCoV) stimulatean organism to generate a specific antibody.

The present invention discloses a black attapulgite medicine bath mud powder. Its composition contains the following components: attapulgite clay, semi-finished product of attapulgite Chine medicinal material composite and distillers' grains. Said black attapulgite medicine bath mud powder contains rich protein, amino acids, vitamins, several minerals, trace elements, organic substances and Chinese medicinal materials. Said invention also provides its application method and its preparation method.

The invention discloses an HCV (hepatitis C virus) core antigen, which is a polypeptide encoded by a DNA (deoxyribonucleic acid) of which the amino acid sequence is shown in SEQ ID NO.1 and the nucleotide sequence is shown in SEQ ID NO.2. The invention also discloses a hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC16-H6 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5074. The invention also discloses another hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC23-G5 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5075. The invention also discloses a monoclonal antibody of an anti-HCV core antigen, which is secreted by the hybridoma cell line (preservation number: CGMCC No. 5074) or the hybridoma cell line (preservation number: CGMCC No. 5075), and has a specificity to the polypeptide shown in SEQ ID No.1.

The invention discloses formamide and isonitrile compounds serving as influenza A virus inhibitors and preparation and application thereof. The structural formulae of the formamide-isonitrile compounds serving as the influenza A virus inhibitors are shown as a formula I and a formula II described in the specification, wherein R is selected from alkyl, naphthenic group, aromatic group, heteroaromatics group, amino acid group and substitutive derivatives of all the groups; the substituent groups of the substitutive derivatives are alkyl, naphthenic group, aromatic group, heteroaromatics group and amino acid group. The formamide and isonitrile compounds are prepared from amine serving as a raw material by means of acylation, dehydration and the like. These formamide and isonitrile compounds and pharmaceutically-acceptable salts thereof can be used for preparing anti-influenza medicaments of which the activities are much higher than that of an existing anti-influenza medicament, namely, amantadine, and the drug tolerance generated by existing compounds can be overcome.

The invention discloses a heat-resistant formate dehydrogenase gene, its coded polypeptide and a preparation method. Based on Bacillussp., the invention clones and isolates the heat-resistant formate dehydrogenase gene, which is useful for the preparation of It is useful to obtain heat-resistant formate dehydrogenase transgenic microorganisms, animals and plants, and recover the enzyme encoded by the gene. In addition, the present invention also provides the amino acid sequence and functional equivalent of the polypeptide having heat-resistant formate dehydrogenase activity. At the same time, the invention also provides a method for preparing, separating and purifying the polypeptide with heat-resistant formate dehydrogenase activity.

The invention provides a biochar-based organic fertilizer and a production method thereof. The biochar-based organic fertilizer is characterized by being prepared from the following components in parts by weight: 20-30 parts of biochar, 35-55 parts of organic matters, 6-9 parts of nitrogen (N), 3-5 parts of phosphorus (P2O5), 5-7 parts of potassium (K2O), 0.5-1 part of amino acids, 0.5-1 part of humic acid, 1.5-2.5 parts of wood vinegar, 1-2 parts of wood tar, 0.05-1 part of a beneficial inoculant, 0.5-1 part of a fertilizer controlled-release agent and 1-3 parts of a forming auxiliary agent.The organic matters are solid animal dung and thoroughly decomposed straws, the water content of which is smaller than or equal to 40%. The production method comprises the following steps: preparing biochar from crop straws after high-temperature pyrolysis; mixing the biochar with the solid organic matters to ferment together for 5-10 days, wherein no biogas slurry is discharged; adding the components in proportion to proportion and granulate; externally spraying the wood tar; and drying the mixture to obtain the biochar-based organic fertilizer. The method solves the problem that the crop straws and the animal dung are accumulated, and does not generate new polluting sources. The organic fertilizer contains various nutritional components and microelements and improves soil, so that wasteresources are utilized effectively to the maximum extent.

The invention relates to a recombinant chimeric antigen of treponema pallidum. The recombinant chimeric antigen is formed by connecting an amino acid sequence as shown in SEQ ID NO.12, a first flexible linker, an amino acid sequence as shown in SEQ ID NO.9, a second flexible linker and an amino acid sequence as shown in SEQ ID NO.13 in series. The recombinant chimeric antigen can be used for serological detection of treponema pallidum antibody, and has improved dissolving expression capability, activity and thermal stability. The invention also relates to a method for preparing the recombinantchimeric antigen.

The invention relates to the field of molecular biology, and in particular to catalpa bungei CabuAP3 protein as well as an encoding gene and application thereof. The amino acid sequence of the catalpa bungei CabuAP3 protein is as shown in SEQ ID No.1; the sequence (CDS) of the encoding gene is as shown in SEQ ID No.2; the whole length of the cDNA sequence of the catalpa bungei CabuAP3 gene is as shown in SEQ ID No.3. The catalpa bungei CabuAP3 gene provided by the invention is only expressed in petals and stamens of catalpa bungei, and is not expressed in leaves, sepal or pistil. A CabuAP3 gene expression vector is transferred into mutants of wild arabidopsis and arabidopsis ap3-3, the result shows that the transgenic wild arabidopsis has one extra petal, which indicates that the CabuAP3 gene has the function of regulating and controlling increase of the number of petals of the transgenic wild arabidopsis, and the transgenic wild arabidopsis ap3-3 mutant has a filament structure. The plant variation material obtained from the catalpa bungei CabuAP3 gene expression vector can be applied to plant breeding and view admire, and has very good application prospect.

An immunological measurement is performed using anti-CTP antibody characterized by recognizing an antigenic determinant included in the polypeptide represented by amino acid numbers 118-132 of SEQ ID NO: 1, and reacting with native Cochlin-tomoprotein (CTP).

The present invention discloses diamondback moth cecropin 3, a preparation method and an application thereof, wherein the diamondback moth cecropin 3 gene has a nucleotide sequence represented by SEQIDNO:1-2 and has a corresponding amino acid sequence represented by SEQIDNO:3, and sequence analysis results show that the mature peptide of the diamondback moth cecropin 3 has an amino acid sequence represented by SEQIDNO:4. According to the present invention, the diamondback moth cecropin 3 gene or the mature peptide sequence thereof is subjected to prokaryotic expression to obtain the diamondback moth cecropin 3, and the obtained diamondback moth cecropin 3 provides strong inhibition effects for gram-negative bacteria and gram-positive bacteria, provides strong inhibition effects for pathogenic fungi, especially provides strong inhibition effects for a lot of pathogenic fungi of melons and fruits, and is expected to be a novel peptide antibiotic.

The present invention belongs to the technical field of feeds for sheep and relates to a paper mulberry silage feed and a preparation method thereof. The paper mulberry silage feed is mainly preparedfrom the following raw materials in parts by weight: 60-75 parts of paper mulberry leaves, 20-35 parts of corn stalks, 18-25 parts of Guicao No. 1 Wangcao (a kind of Chinese pennisetum), 20-25 parts of feed mulberry leaves, 6-10 parts of canterburybells, 2-4 parts of compound minerals, 1-2 parts of amino acids, 0.8-1.5 parts of edible salt, 6-10 parts of sugar and 1-3 parts of a fermenting agent;and the fermenting agent is a mixture of saccharomonospora viridis, neurospora sitophila, clostridium thermocellum, fusariwn oxysporum and chaetomium globosum, wherein the weight ratios of the saccharomonospora viridis, neurospora sitophila, clostridium thermocellum, fusariwn oxysporum and chaetomium globosum are (2-3):(2-3):(3-6):(2-4):(1-3). The paper mulberry silage feed has effects on improving digestion and absorption, improving palatability, proliferating bifidobacteria and enhancing immunity. Besides, the provided preparation method is simple and easy to popularize.