9 results about "A-DNA" patented technology

The invention discloses an HCV (hepatitis C virus) core antigen, which is a polypeptide encoded by a DNA (deoxyribonucleic acid) of which the amino acid sequence is shown in SEQ ID NO.1 and the nucleotide sequence is shown in SEQ ID NO.2. The invention also discloses a hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC16-H6 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5074. The invention also discloses another hybridoma cell line secreting monoclonal antibodies, the hybridoma cell line is named as SC23-G5 and has been preserved in the China General Microbiological Culture Collection Center on July 14th, 2011, and the preservation number is CGMCC No. 5075. The invention also discloses a monoclonal antibody of an anti-HCV core antigen, which is secreted by the hybridoma cell line (preservation number: CGMCC No. 5074) or the hybridoma cell line (preservation number: CGMCC No. 5075), and has a specificity to the polypeptide shown in SEQ ID No.1.

The invention relates to a method for extracting microorganism total DNA from liquid milk. The extraction method comprises a cell lysis process, a DNA purification process, a DNA recovering process and a DNA dissolving process. The method for extracting the microorganism total DNA from the liquid milk has the following beneficial effects: less time is consumed; steps are few; waste of lysate is avoided; the quality of the extracted DNA is purer than that of DNA extracted according to the traditional method, the quality is higher, and the quantity of the extracted DNA is larger than that of thetraditional method.

The invention discloses a DNA (deoxyribonucleic acid) barcode reference gene sequence of Trogia venenata belonging to poisonous mushrooms. The barcode reference detection gene is a TEF gene in a gene sequence shown as SEQ ID NO.1. PCR (polymerase chain reaction) is carried out for amplification to obtain a sample TEF gene, PCR products are verified by agarose gel prior to being sent to a biological sequencing company for sequencing, and after manual checking, sequence assembling and comparison of a sequencing result, a to-be-tested tissue can be determined as T.venenata if homology between the sequencing result and the gene sequence SEQ ID NO.1 is above 99%. By means of reliable DNA molecular identification, time consumption for morphological identification is avoided, and quickness and accuracy in identification of the trogia venenata are realized.

The object of the present invention is to obtain a novel transposon-like element specifically present in the genome of rye and the like. According to the present invention, a DNA sequence of a transposon-like element Revolver comprising 3,041 nucleotide pairs, and DNA sequences of structural mutants thereof were provided. The DNA sequence of the transposon-like element Revolver, the DNA sequences of genes having transcriptional activity encoded by Revolver, and the DNA sequences of structural mutants thereof can be utilized for detection of a genome, development of DNA markers, identification of chromosomes, a probe for study on evolution, an entry point of PCR and the like, in useful resource plants of Poaceae.

The invention discloses an automatic detection device for pine wood nematode DNA. The device comprises a DNA detection fixed homogenizing cylinder assembly, a homogenizing chassis assembly is fixedlyarranged at the bottom of the DNA detection fixed homogenizing cylinder assembly, and a DNA detection movable homogenizing cylinder assembly is arranged at a position opposite to the DNA detection fixed homogenizing cylinder assembly in a sliding manner. The DNA detection fixed homogenizing cylinder assembly and the DNA detection movable homogenizing cylinder assembly are separated by outwards pulling a push-pull ball; then, a beaker or a test tube is inserted between the DNA detection fixed homogenizing cylinder assembly and the DNA detection movable homogenizing cylinder assembly through a homogenizing cavity; a homogenizing motor drives the DNA detection fixed homogenizing cylinder assembly and the DNA detection movable homogenizing cylinder assembly to rotate, so that homogenizing operation during early-stage material preparation and subsequent detection treatment in the beaker or the test tube is realized; and vibration during rotary homogenizing treatment is realized through elastic buffering and bidirectional rotation, and the homogenizing efficiency and effect are ensured.

The invention relates to a DNA library for detecting and diagnosing corneal dystrophy disease-causing genes as well as application of the DNA library. The library comprises 24 disease-causing genes related to corneal dystrophy; 24 corneal dystrophy disease-causing genes are preferably selected, a probe pool is designed, a target area library aiming at the 24 corneal dystrophy disease-causing genesis established, and the library performs sequencing by a high-throughput sequencing technology, looks for disease-cause mutation and provides genetic and molecular biological basis for clinical diagnosis. The library has the characteristics of accuracy, rapidness, flexibility and low cost; 24 gene detection areas can detect various kinds of common corneal dystrophy such as epithelial and subepithelial corneal dystrophy, Bowman's membrane corneal dystrophy, stromal corneal dystrophy, descemet's membrane and corneal endothelium dystrophy; and important significance and clinical value in diagnosis and differential diagnosis on the corneal dystrophy are achieved.