31 results about "Blood serum" patented technology

The present invention relates to a method for processing human placental cell sample, a human placental cell sample obtained according to said method for processing human placental cell sample, a human placental cell bank, a method for banking human placental cells, a method for searching human placental cell sample in said human placental cell bank according to the present invention, a method for preparing human cord blood serum, use of human placental cells obtained by said method for processing human placental cell sample or human placental cell bank established by said method for banking human placental cells in treating human dysfunction and diseases due to cell injury or cell malfunction, as well as a method for treating human dysfunction and diseases due to cell injury or cell malfunction.

The invention relates to the technical field of cell culture, and in particular to a serum-free culture medium, a preparation method thereof and a culture method of mesenchymal stem cells. The culturemedium consists of the following components: non-essential amino acid, glutamine, hydrocortisone, dexamethasone, polyvinyl alcohol, recombinant human insulin, recombinant human transferrin, a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human Wnt-3a protein, a recombinant human fibronectin, recombinant human laminin, L-glutathione, L-ascorbic acid, beta-mercaptoethanol, ethanolamine, Pluronic F-68, Tween 80, cholesterol, adenine, sodium selenite and a basic culture medium. According to the invention, polyvinyl alcohol can replaceserum albumin in a serum-free culture medium in carrier function, so that proliferation capacity of mesenchymal stem cells is effectively improved. Polyvinyl alcohol has more stable performance thanserum albumin, and the problem that proliferation capacity and passage capacity of stem cells are reduced can be effectively solved.

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

The invention discloses a traditional Chinese medicinal composition for treating hepatitis and a preparation method thereof. The traditional Chinese medicinal composition for treating the hepatitis is prepared from the following raw materials: pseudo-ginseng root, golden thread, baical skullcap root, amur corktree bark, rhubarb, spreading hedyotis herb, snake gall, borneol, calculus bovis factitius, aritificial musk, curcuma aromatica and red yeast. The traditional Chinese medicinal composition for treating the hepatitis can be prepared into multiple common formulations for oral administration. The traditional Chinese medicinal preparation treats the hepatitis by clearing heat, detoxicating, removing blood stasis, eliminating stagnation, invigorating blood circulation, dissolving stasis, detoxicating and normalizing gallbladder, has the obvious effects of improving liver function and inhibiting liver injury and hepatitis virus, addresses both symptoms and root causes, requires a short treatment period, realizes a low relapse rate, and has the obvious pharmacological action of protecting the liver and reducing transaminase and serum bilirubin.

The invention relates to a blood sampling auxiliary device for separating a serum sample from whole blood, at least comprising: a blood sampling tube body for storing collected liquids; a blood sampling tube cover assembled on the blood sampling tube body; and a blood sampling and subpackaging assembly capable of controllably conveying a liquid in the blood sampling tube body out of the blood sampling tube body in the mode that one end of the blood sampling and subpackaging assembly is connected with the blood sampling tube cover in a matched mode. The blood sampling and subpackaging assemblyis configured to output a liquid stored in the blood sampling tube body by times in a specified amount in a manner that the gas from the outside of the blood sampling tube body through the blood sampling tube cover does not pass through the liquid stored in the blood sampling tube body.

Methods for administering pressure changes to a user for the treatment and prevention of diseases and conditions are disclosed herein. Methods of administering Cyclic Variations in Altitude Conditioning Sessions (CVAC Session(s)) for the treatment of steroidogenesis, steroid levels, and treatment of serum lipid levels are disclosed herein. Also disclosed herein are methods of administering CVAC Session(s) for the treatment of steroid levels and steroidogenesis associated with HIV infection.

The invention relates to an electrochemical immunosensor of an electroactive substance modified MOF composite material as well as preparation and application of the electrochemical immunosensor. According to the electrochemical immunosensor, a Cu < 2 + > modified metal-organic framework composite material and a [Fe (CN) 6] < 3-> modified metal-organic framework composite material are used as signal tags for simultaneously detecting multiple components; and the immunosensor platform is prepared from the Au/rGO composite material. The electrochemical immunosensor provided by the invention has the advantages of wide linear range, high sensitivity, good selectivity, greenness, simplicity and the like, provides an effective method for simultaneously detecting two biomarkers of acute myocardial infarction (AMI) in clinical serum, has certain application potential in the aspect of early accurate diagnosis of AMI, and has a bright application prospect; and the method has enlightening significance on detection of other related biomarkers.

The invention discloses a detection method of an antiphospholipid syndrome related antibody. The detection method combines a microsphere analysis technology and a flow analysis technology, and performs joint detection on 12 antiphospholipid antibody indexes such as aCL-IgG, aCL-IgM, aCL-IgA, beta2-GPI-IgG, beta2-GPI-IgM, beta2-GPI-IgA, PS-IgM, PS-IgG, PI-IgM, PI-IgG, PI-IgM, PI-IgG and the like. The detection method comprises the following steps of: 1) preparing a serum sample; 2) preparing and activating microspheres; 3) preparing a capture antibody; 4) carrying out coupling; 5) performing antigen-antibody combination; 6) carrying out fluorescence labeling reaction; and 7) performing flow type detection. Compared with an existing conventional clinical detection method, the detection method has the advantages that joint detection of 12 antiphospholipid marker antibody indexes for diagnosing the anti-phospholipid syndrome is realized, the required sample size is smaller, the operation time is shorter, multi-parameter analysis can be performed, meanwhile, the specificity and the sensitivity are further extremely high, and the detection level and the standardization degree of the anti-phospholipid antibody index are further improved.

The invention relates to a pharmaceutical composition for treating serotonergic neurotransmission related disease or condition, including an effective amount of a chlorophenylpiperazine derived compound of Formula (I),

or the pharmaceutically acceptable salt thereof. The pharmaceutical composition is safe and does not have side effect on lethargy.

The invention relates to an RPA-LFD primer, a probe and a kit for jointly detecting an epidemic hemorrhagic disease virus and a Palimer serum group virus, and belongs to the technical field of molecular biological detection of animal viruses. The primer and the nfo probe for jointly detecting EHDV and PALV are designed, the RPA-LFD detection kit capable of jointly detecting the nucleic acids of EHDV and PALV popular in China is constructed on the basis, and the kit has the advantages of specificity, sensitivity, rapidness, high efficiency, isothermal amplification, on-site rapid diagnosis and the like, and is easy to popularize and apply.

The invention discloses a novel serum-free cell cryopreservation liquid formula which comprises the following components: human serum albumin as a main body, 10% of DMSO (dimethylsulfoxide), 13.3% of compound electrolyte injection and 16.70% of dextran. By optimizing the formula of the cryopreservation liquid, the survival rate of the cells is remarkably improved, the pollution source is reduced, the novel serum-free cell cryopreservation liquid is provided, the serum-free cell cryopreservation liquid is easy to prepare and store, the viability of the recovered cells is extremely high, and potential risks existing in serum are avoided.

The invention discloses an application of porphyrin in tin in preparation of medicines for treating thalassemia, which uses porphyrin in tin for intraperitoneal injection of beta thalassemia mice, and finds that porphyrin in tin can enable invalid red systems of thalassemia mice to return to normal, so that alpha/beta globin chain imbalance of red blood cells is effectively relieved, and the curative effect of thalassemia is improved. The red blood cell life, the total red blood cell number and the hemoglobin level are improved; meanwhile, the iron content and splenomegaly of serum and liver are reduced. After porphyrin in tin is used for treatment, invalid hematopoiesis, anemia and iron overload of thalassemia mice are effectively relieved, no obvious toxic or side effect exists, treatment is convenient to implement, and the curative effect can be kept only by maintaining low dosage after high dosage.

The invention provides a method for preparing recombinant protein. The method comprises a growing stage and a production stage, wherein the production stage comprises the following steps: S1, culturing mammalian cells expressing recombinant protein in a serum-free culture medium; S2, collecting supernatant; S3, culturing the supernatant in a serum culture medium for 12-48 hours; and S4, replacingthe serum culture medium with a serum-free culture medium, and repeating S1-S2 for one or more times. The method at least has the following beneficial effects that, the serum-free culture medium is replaced by the serum culture medium after one round of supernatant collection from cells in a bioreactor is completed in the production stage, the state and vitality of cells can be quickly restored byutilizing ingredients beneficial to cell growth in serum, subsequent round of protein production can be constantly performed, and considerable production efficiency can be maintained, so that the cells at the terminal stage of the same round of growth stage can realize production of recombinant protein in multiple rounds and multiple batches in the same bioreactor, and one round of cell output efficiency can be effectively improved.

The invention is suitable for the technical field of medicine and food health care, and provides a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof. The traditional Chinese medicine composition comprises ampelopsis grossedentata, chitosan oligosaccharide, hovenia dulcis thunb and cyclocarya paliurus. The mass ratio of the ampelopsis grossedentata to the chitosan oligosaccharide to the hovenia dulcis thunb to the cyclocarya paliurus is (30-60): (0.5-1): (10-30): (10-30). The traditional Chinese medicine composition has obvious effects of dispelling the effects of alcohol and protecting the liver, can remarkably reduce the drunkenness rate of mice with acute alcoholism, shortens the sleep maintenance time and the sobering time, and prolongs the drunkenness incubation period; meanwhile, abnormal rise of ALT and AST levels in serum of mice can be inhibited, abnormal rise of MDA level in the liver can be inhibited, the ADH and GSH activity in the liver can be enhanced, a remarkable liver protection effect is achieved, and a good relieving effect on acute alcoholism caused by drinking a large amount of alcohol is achieved; the raw materials are wide in source, the preparation process is simple, and the cost is low.

The invention relates to an implant surface activity treatment method capable of promoting healing of a dental implant, the implant surface activity treatment method comprises the following specific operation steps: drawing blood from an implant patient to an additive-free negative pressure tube, then installing the negative pressure tube in a limiting mechanism of a centrifugal machine, centrifuging under the conditions of 15 minutes and 3000 revolutions, and after the centrifugation is finished, enabling the guide mechanism to be matched with the rubber head dropper to take out serum in the negative pressure tube, and taking out the implant and immersing in the serum and then implanting; the serum contains PDGF and VEGF components which can promote rapid vascularization and shorten the healing period, the operation is easy, completion is not needed in a vacuum environment, the surface activation rate is high, the actual activation rate is 83%, and the healing acceleration can be advanced by 1.5-2 months on the aspect of the healing effect.

The serum-free culture of normal human epithelial stem cells is of paramount importance for the in vitro formation of cloned human tissues by means of cell therapy. Several growth promoting agents are disclosed for use in a serum-free culture medium of normal human keratinocytes. Lithium ions, dibutryl-cyclic adenosine monophosphate, and prostaglandin E1 have been found effective as growth enhancing agents to be added singly or in combination, when used in combination with insulin-like growth factor-1. Lithium ions and prostaglandin E1 are disclosed as independent growth enhancing factors, that replace epidermal growth factor as a necessary growth factor required for keratinocyte clonal growth.

The invention discloses a drainage type kit for early diagnosis of diabetic nephropathy, which comprises a kit body, a constant temperature block is fixedly connected in the kit body, the inner wall of the kit body is connected with a plurality of circularly distributed isolation detection components, and the isolation detection components can be separated and detected through the cooperation of the constant temperature block and a negative pressure drainage component. The sealing detection mode can be effectively achieved, serum in the serum storage tank can make contact with the sealed isolation detection assembly for detection under drainage of the negative pressure drainage assembly, it is effectively avoided that the serum and detection test paper are exposed to the external environment in the detection process, the detection error is reduced, and the reliability of the detection result is improved; the detection steps are effectively simplified, the detection difficulty is reduced, the kit is convenient for common personnel to operate and use, and the popularization of the kit body is effectively promoted.

A microfluidic blood serum generator having a first port and a second port, between which there is formed a microfluidic structure, wherein the microfluidic structure has a supply duct communicating with the first port, a clotting region into which the supply duct discharges, and a discharge duct which communicates with the second port and into which the clotting region transitions, wherein the first port is designed to introduce a blood sample at a first pressure and the second port is designed to apply a second pressure that is lower than the first pressure, wherein the clotting region is formed by a single, coherent cavity, and wherein a barrier for retaining a clotted blood sample is arranged in the transition from the clotting region to the discharge duct. A method for separating off blood serum from a blood sample using a blood serum generator of this kind.

The invention belongs to the technical field of cell culture, and particularly relates to a knockout serum replacement for cell culture and a preparation method thereof, a knockout serum replacement composition for cell culture and a cell culture medium. The knockout serum replacement for cell culture comprises, by weight, water, 18.5-67 parts of amino acid, 200-815 parts of protein, 62.5-252 parts of inorganic salt, 1.0-3.8 parts of ethanol amine, 50-200.0 parts of sodium pyruvate, 1.25-5.0 parts of ascorbyl phosphate, 0.5-2.0 parts of glutathione and 5.0-20 parts of insulin. The knockout serum replacement for cell culture can be added into any one cell culture medium, the adding amount of serum is reduced, and therefore, the cost is saved. The knockout serum replacement for cell cultureis suitable for protein recombination, vaccine production and other kinds of cell culture, and high in universality.

The invention belongs to the technical field of biomedicine and particularly relates to virus carrier particles, a construction method and application thereof to preparation of medicine for treating human chronic pain. The virus carrier particles comprise pspax2 particles, pmd2.G particles and pwpi particles, and the pwpi particles comprise promoters, creotoxin enzyme activity areas and internal ribosome entry site sequences. The construction method of the virus carrier particles includes the steps that 1, a cell line lenti-x293T is cultured; 2, a DMEM culture medium is adopted; 3, the pspax2 particles, the pmd2.G particles and the pwpi particles are subjected to transfection into lenti-x293T cells; 4, transfection liquid is washed off, and culture liquid containing 10wt% calf serum is added for culturing; 5, the culture liquid is recycled, filtered and centrifuged, and the virus carrier particles high in drop degree can be obtained. The virus carrier particles are more targeted to treatment of chronic pain and can prevent vicious circle between a nervous system and peripheral cells.

A cell-penetrating peptide has remarkably excellent cell penetration efficiency, and is capable of penetrating 100% into all cells within 1 hour even in an environment including serum or sera, as well as a stable structure. Therefore, it is possible to move a cargo into the cell while maintaining the function of the cargo without damaging the same.