Virus carrier particles and construction method and application thereof

A technology of virus vector and construction method, applied in the field of biomedicine, can solve the problems of no toxin effect, inability to inhibit stimulation well, and achieve the effect of reducing secretion

Active Publication Date: 2016-05-11
郑州可尔利尔生物科技有限公司
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rheumatologists have found Botox not only for tension headaches/migraines but injecting the toxin into the joints is also effective in RA (rheumatoid arthritis) and OA (osteoarthritis) patients who do not respond well to other medications However, because the endothel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Virus carrier particles and construction method and application thereof
  • Virus carrier particles and construction method and application thereof
  • Virus carrier particles and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A lentiviral vector particle includes pspax2, pmd2.G and pwpi plasmids. The pwpi plasmids include a promoter, a botulinum toxin type D enzyme active region and an internal ribosome entry site (IRES) sequence.

[0048] The synthetic botulinum toxin D-type enzyme active region gene is designed with a region for enhanced expression (ACCATGGGC), which is located before its initial coding, and contains PmeI sites for cloning, at the 5'and 3'ends, see the sequence table In the sequence 1. After botulinum toxin type D enzyme active region gene was digested and recovered by PmeI, it was cloned into pwpi plasmid after PmeI digestion and alkaline phosphatase treatment. The correct cloning direction was confirmed by PstI digestion and gene sequence determination.

[0049] The construction method of the above pwpi plasmid includes the following steps:

[0050] (1) First, 1μg of pwpi vector containing the marker gene (GFP or RFP) was digested with 1μLPME1, and digested in a 50μLeppendorf tu

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biomedicine and particularly relates to virus carrier particles, a construction method and application thereof to preparation of medicine for treating human chronic pain. The virus carrier particles comprise pspax2 particles, pmd2.G particles and pwpi particles, and the pwpi particles comprise promoters, creotoxin enzyme activity areas and internal ribosome entry site sequences. The construction method of the virus carrier particles includes the steps that 1, a cell line lenti-x293T is cultured; 2, a DMEM culture medium is adopted; 3, the pspax2 particles, the pmd2.G particles and the pwpi particles are subjected to transfection into lenti-x293T cells; 4, transfection liquid is washed off, and culture liquid containing 10wt% calf serum is added for culturing; 5, the culture liquid is recycled, filtered and centrifuged, and the virus carrier particles high in drop degree can be obtained. The virus carrier particles are more targeted to treatment of chronic pain and can prevent vicious circle between a nervous system and peripheral cells.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner 郑州可尔利尔生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap