The invention relates to a novel application of
small molecule metabolites 2-piperidone and
sphingomyelin (SM) 30: 1 in a serum sample as a combined marker in preparation of a kit for diagnosing children
asthma. The invention further relates to a kit for detecting
asthma in a subject, and whether the subject suffers from
asthma or not is judged by detecting the respective concentrations of the combined markers in serum of the subject, calculating the variable Prob of the combined markers based on a binary
logistic regression equation and judging an intercept point value. The kit has the characteristics of low detection cost and good stability. The kit can be used for assisting
clinical diagnosis of asthma, has the characteristics of high diagnosis specificity and high sensitivity, and has relatively high development and application values.