28 results about "Small molecule" patented technology

The use of porous monolithic matrix for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis on a sample suspected of containing an analyte is herein described. The porous polymer monoliths provide a matrix suitable for use in detecting and analyzing low molecular weight and small molecules. The matrices described herein also facilitate the creation of arrays of monolithic matrices for high-throughput detection and screening using MALDI-TOF mass spectrometry and exhibit a long shelf-life.

The computerized method, software, and product of this invention implements a process for computationally designing proteins and/or associated molecular targets with desired quantitative stability/flexibility relationships (QSFR). QSFR is an innovative, high dimensional metric calculated using an improved Distance Constraint Model (DCM). This invention includes three distinct aspects: (i.) utilizing QSFR in computational molecular design of protein sequences, or variations of molecular structures, to have specific stability and flexibility relationships deemed important for biochemical function for specified thermodynamic and solvent conditions; (ii.) utilizing QSFR to optimize thermodynamic and/or solvent conditions necessary for a specified molecular system to exhibit protein function characteristics, such as improved catalytic efficiency; and (iii.) utilizing QSFR to computationally screen or design small molecules and/or design its putative receptor so as to exhibit a desired affect on protein function.

Methods, reagents, compositions, and kits for in situ interaction determination (ISID) via interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. ISID technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution. ISID is compatible with unpurified targets in biological samples and can be used to evaluate ligand-binding in DNA-encoded chemical libraries in cell lysates. ISID is also useful to screen ligand interactions of proteins or other molecules in their native state, including their native post-translational modifications and any interactions with accessory proteins and metabolites, in ways that better reflect their relevant biological environment. Because ISID is compatible with crude cell lysates, difficult-to-purify, poorly soluble, intrinsically unstable, and aggregation-prone targets may also be compatible with this method, without requiring truncation or other strategies used to promote heterologous expression.

The invention discloses an inorganic sensitive layer based surface enhanced Raman spectrum detection technology and material preparation thereof. According to the detection technology, a surface enhanced Raman spectrum material based on an inorganic sensitive layer is used as an active substrate of a surface enhanced Raman scattering, and is exposed in the small molecule atmosphere; a new solid substance formed by specific reaction of the inorganic sensitive layer and small molecules to be detected by a Raman spectrometer is detected, and the type of the small molecules is obtained according to the Raman spectrum of the new solid substance; according to the preparation method, a colloid electrostatic self-assembly method, a thiourea-induced isotropic shell growth method, a metal halogenation method and a physical deposition method are used for a precious metal nanoparticle colloidal solution, a metal salt solution and a thiourea solution to prepare the material in which precious metalnanoparticles are coated with a metal oxide layer, or the precious metal nanoparticles are coated with a metal sulfide layer, or the precious metal nanoparticles are coated with a metal halide layer.The technology can be used for real-time online detection of hydrogen sulfide, carbon disulfide, hydrogen chloride, mustard gas, nitrogen dioxide and the like.

The invention relates to a production method for preparing polyvinyl chloride fibers for hair by a melting method, which belongs to the production field of polyvinyl chloride fibers. The production method is realized through the following steps: (1) mixing PVC resins, a plasticizer, a stabilizing agent, a lubricant, an antioxygen agent, and organic montmorillonite according to a certain proportion; (2) performing melting and spinning by a screw extruder; (3) stretching fibers spun in the step (2), processing the stretched fibers by an extraction agent, further stretching the processed fibers so as to obtain dimensionally stable PVC fibers for hair; (4) recycling the plasticizer separated by distilling residual treating liquids, and the extraction agent with a low boiling point. The residual volume of the plasticizer in the PVC fibers prepared by the production method disclosed by the invention is small, the migration of small molecules during the use of products is effectively avoided, and the contractility of the fibers is greatly lowed, so that the lustrousness of the fibers is more similar to that of human hair. The technological method is simple, the cost is saved, and continuous high-efficiency production can be realized.

The invention discloses a method for refining a high-temperature wood hydrolyzate. The method comprises the following steps: performing centrifugation on a raw material hydrolyzate; removing the water-soluble macromolecular lignin by a flocculation method; obtaining the hydrolyzate after flocculation, adsorbing the water-soluble small molecule lignin with the resin, and obtaining a purified xylosesolution; removing residual lignin of the purified xylose solution by a filtering device to obtain refined xylose liquid and prepare xylose; and eluting the resin-adsorbed lignin with an eluting solvent, and recovering the eluting solvent and lignin. The recovered ethanol of the invention can be recycled and reused; an integrated vortex reactor is also used to effectively improve and increase theflocculation effect, and the ion exchange resin as an adsorbent has a good adsorption effect on the charged lignin. The hydrolyzate improves the membrane separation effect of a filter membrane and maintains a large flux of the membrane.

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

The invention relates to the field of biological detection and the field of nanomaterial functional application, and discloses a method for preparing a core-shell SERS structure based on the amplification of a nucleic acid strand by terminal deoxynucleotidyl transferases. The method comprises the following steps of: assembling a starting strand on the surface of a nanomaterial, carrying out the DNA amplification reaction on the surface of the terminal deoxynucleotidyl transferase, transferring deoxynucleotide to the starting primer strand assembled on the surface of the nanomaterial so as to form long single-stranded DNA on the surface of the nanomaterial and then obtain a nanomaterial-long-single-stranded-DNA complex; and covering a metal shell layer outside the nanomaterial-long-single-stranded-DNA complex by the chemical reduction method so as to form the core-shell structure. The core-shell SERS structure has long strand DNA which can be used as a Raman reporter small molecule so as to output efficient and uniform SERS signals to enhance the intensity of Raman signals. Bases are located between the core and the shell, so that the number of ''hot spots'' in the core-shell structure is increased, the adsorption of target molecules or antibodies on the surface of the core-shell structure is affected, the sensitivity of the detection can be greatly improved, and the method canbe applied to the field of biological analysis and detection.

The invention discloses a small micelle nano-drug and a preparation method and application thereof. A micromolecule drug and an amphiphilic polymer/targeted amphiphilic polymer are added into polycthylene glycol oilgomers to obtain a mixed solution, then the mixed solution is added into a buffer solution to obtain the small micelle nano-drug; and the molecular weight of the polycthylene glycol oilgomers is 200-600. The invention designs and prepares an efficient paclitaxel PTX-loaded micelle MPTX, a disulfide cross-linked micelle MPTX and three TNBC active targeting micelle nano-drug, the drug loading capacity is up to 23.1 wt.%, the particle size is less than 40 nm, and the micelle nano-drug has good stability and drug release performance of reduction response.

The invention provides a method for detecting polymer impurities in a cefadroxil bulk drug or a preparation thereof, which is based on reversed-phase high-performance liquid chromatography. The method comprises the following steps that chromatographic conditions are established, and a polymer impurity positioning control solution is prepared, wherein the chromatographic conditions are as follows, a stationary phase is octadecyl silane bonded silica gel, a mobile phase is 0.02 mol/L monopotassium phosphate solution with the pH value of 4.8-5.2 as a mobile phase A, methanol is taken as a mobile phase B, and gradient elution is performed; the flow rate is 0.9 to 1.1 ml/min; the column temperature is 28-32 DEG C; the detection wavelength is 220nm. According to the method for detecting the polymer impurities in the cefadroxil bulk drug or the preparation of the cefadroxil bulk drug, multiple polymer impurities can be detected at the same time, the separation degree of all the polymer impurities is good, and the method can eliminate interference of small molecule impurities in the cefadroxil bulk drug or the preparation of the cefadroxil bulk drug.

The invention discloses nutritional noodles added with fish polypeptide powder and a preparation method of the nutritional noodles added with fish polypeptide powder. The nutritional noodles comprisethe fish polypeptide powder and flour, wherein the fish polypeptide powder is obtained by fermenting fish leftovers with bacillus velezensis MRS with the preservation number being CGMCC No.18065 and then performing enzymolysis with alkaline protease. The preparation method of the nutritional noodles is simple, peptides in fishes directly enter cells in the form of small molecule peptides to promote nutrient absorption, and the fish polypeptide powder is added into the noodles, so that the taste of the noodles is enriched, the noodles are endowed with higher nutritional value, the fish leftovers are fully utilized, material waste is avoided, cohesiveness, elasticity and toughness of the noodles are enhanced, and viscoelasticity and strength of the noodles are reduced.

The invention discloses a polynitrogen heterocyclic super molecule sensitizer and an application thereof, wherein the sensitizer uses a polynitrogen heterocyclic derivative as an electron donor of thesensitizer, the polynitrogen heterocyclic super molecule sensitizer is formed by an intermolecular hydrogen bond between-N= group and a carboxylic acid small molecule, the structural formula is as shown in the specification, wherein ''suspension points'' represent the intermolecular hydrogen bond. The polynitrogen heterocyclic super molecule sensitizer has simple molecular assembly and strong stereostructure, and can be used for preparing a solar cell with high photoelectric conversion efficiency.

The invention relates to a hydrolysis saccharification process of alternanthera philoxeroides. Metal substance pollutants (iron, cobalt and nickel) in alternanthera philoxeroides raw materials are absorbed by adopting magnetic force, so that subsequent mechanical abrasion and contents of metal ions in treating fluid are reduced, and a good fermentation condition is created for detoxification of selected cupriavidus metallidurans; then solid-liquid separation is realized by adopting an extrusion method, hydrolysis on lipids and protein contained in liquid-state materials is carried out by adopting an NaOH solution, and then hydrolysis treatment is further carried out by adopting trypsase, so that the hydrolysis is more completely carried out; meanwhile, extruded solids are dried and crushedand then dropped into a sulfuric acid solution to carry out acidolysis; through electrorheological fluid shear stress effect treatment, cellulase is then added and enzymatic treatment is carried out,so that the effect is good and energy consumption is low. According to the hydrolysis saccharification process of the alternanthera philoxeroides disclosed by the invention, the raw material treatment efficiency is improved to the maximum extent, and finally, saccharified hydrolytic solutions rich in nutrient substances such as glucose, amino acid, small molecule chain lipids and the like are obtained, so that the glucose content is high, and the glucose can be conveniently supplied for further fermentation, and the production efficiency is improved.

The invention relates to the technical field of chemical small molecule active group chemical modification and medicines, and discloses a preparation method and application of a fusidic acid chemically modified compound. A natural antibiotic, namely fusidic acid, is chemically modified to obtain dihydro-fusidic acid. The invention also discloses application of fusidic acid-containing derivatives in preparation of anti-inflammation medicinal preparations and medicaments, and application of the fusidic acid-containing derivatives in treating skin inflammation caused by bacterial infection, or application of the fusidic acid-containing derivatives in development of anti-inflammation products in preparation of relevant medical apparatuses and instruments. Meanwhile, a new fusidic acid derivative is also provided for preventing, controlling and treating various inflammations in the fields above.

The invention discloses an immunoadsorption blood purification material and a preparation method thereof. The preparation method comprises the following steps: activating a solid-phase carrier by using CDI, and further covalently coupling ligand molecules with amino groups to obtain the immunoadsorption material. The method is simple in process, compared with an immunoadsorption carrier prepared from the same raw materials in the prior art, the immunoadsorption carrier is higher in pathogenic substance clearance rate, ligand molecules are not prone to falling off, and the immunoadsorption carrier can be used for plasma adsorption and can also be directly used for whole blood adsorption; meanwhile, small and medium molecular pathogenic substances such as urea and creatinine can be removed through hemodialysis or hemodialysis filtration. The amino-containing anticoagulant is introduced into the solid-phase carrier, so that the prepared immunoadsorption material has self-anticoagulant property, in the blood purification process, the additional anticoagulant can be reduced or even completely not used, and the risk of bleeding of a patient is greatly reduced.

The invention discloses a bridged tetraphenylethylene (FTPE)-based supramolecular polymer light capture system as well as preparation and application thereof, and belongs to the technical field of photoluminescence nano materials. A quadruple hydrogen bond supramolecular polymer formed by a diureido pyrimidone compound M derived from an FTPE group is used as an energy donor, a small molecular fluorescent dye NDI is used as an energy receptor, and nanoparticles are prepared in a water phase through a microemulsion method to construct the light capture system. The light capture system provided by the invention is constructed in a water phase, and is green and economical; the light capturing capability is high; an adjustable multicolor luminescence property is realized; the structure is stable, aggregation fluorescence quenching after self-assembly is avoided, and a solution thought is provided for aggregation fluorescence quenching of a light capture system in a water phase.

The invention provides an amphiphilic alpha-helical polypeptide for inhibiting PD-L1 (programmed death-ligand 1) and application of the amphiphilic alpha-helical polypeptide. By investigating the in-vitro PD-1/PD-L1 protein binding inhibition effect, the in-vivo and in-vitro anti-tumor effect and the in-vivo immune activation effect of the polypeptide, it is verified that the polypeptide can block a PD-1/PD-L1 signal channel and activate the immune response of T cells and has the anti-tumor effect. The polypeptide disclosed by the invention can be prepared by adopting a solid-phase synthesis method, and compared with a monoclonal antibody and a small molecule compound, the polypeptide disclosed by the invention solves the problems of insufficient immune-related toxicity, pharmacokinetics and tumor permeation, high cost burden of manufacturers and consumers and the like.

Novel leptin and leptin-fusion protein-small molecule drug conjugates are disclosed, where conjugation takes place through the use of cleavable and non-cleavable linkers. The small molecules chemically linked to the protein carrier are imported into the specific leptin-responsive target tissue or cell through receptor-mediated endocytosis and released from the carrier protein through enzymatic cleavage of the linker-drug moiety or through lysosomal degradation of the carrier protein. Free drug then act to modify leptin receptor-triggered biochemical pathways that are altered in conditions of leptin-resistance. Drug will correct altered pathway allowing resensitization of receptor signaling. This can greatly aid in the resolution of pathologies either initiated at the onset of leptin resistance or exacerbated by it.