Smart phone-based mobile instant microbiological diagnosis instrument
一种智能手机、微生物的技术,应用在仪器、科学仪器、电话机结构等方向,能够解决技术转化壁垒、难以适应环境保护、体积大等问题
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Embodiment 1
[0027] Detection of sulfate-reducing bacteria:
[0028] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.
[0029] 100 µL concentration (10 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.
[0030] 100 µL concentration (10 2 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light sour...
Embodiment 2
[0039] E. coli detection
[0040] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.
[0041] 100 µL concentration (10 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.
[0042] 100 µL concentration (10 2 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrum...
Embodiment 3
[0051] Staphylococcus aureus detection
[0052] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.
[0053] 100 µL concentration (10 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.
[0054] 100 µL concentration (10 2 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to...
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