Smart phone-based mobile instant microbiological diagnosis instrument

[0027] Detection of sulfate-reducing bacteria:

[0028] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0029] 100 µL concentration (10 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0030] 100 µL concentration (10 2 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light sour...

[0039] E. coli detection

[0040] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0041] 100 µL concentration (10 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0042] 100 µL concentration (10 2 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrum...

[0051] Staphylococcus aureus detection

[0052] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0053] 100 µL concentration (10 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0054] 100 µL concentration (10 2 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to...