Detecting method for cholesterol metabolites in brain tissue

A detection method, brain tissue technology, applied in the fields of biomedicine and analytical chemistry, to achieve the effect of less sample consumption and high sensitivity

Inactive Publication Date: 2019-01-15
上海博云生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to easily isolate specific chemical compounds from different sources such as blood or cerebrospinal fluid without affecting their healthy cells by analyzing them with high sensitivity techniques like liquid chromato graphic analysis.

Problems solved by technology

This patented technical problem addressed in this patent relates to accurately measuring both choline esters (ChE), carboxylesters (CEL2), nitrosothiocyanine receptor-2(NTRR2), glucoside triene pathway (GTP)-binding protein family member 3a-5b). Current techniques have limitations due to lack sensitivity and accuracy issues associated with these molecules alone.

Method used

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  • Detecting method for cholesterol metabolites in brain tissue
  • Detecting method for cholesterol metabolites in brain tissue
  • Detecting method for cholesterol metabolites in brain tissue

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with the examples. It should be noted that the following descriptions are only for explaining the present invention and not limiting its content.

[0049] 1. Materials

[0050] 1.1 Brain tissue

[0051] Brain Tissue Sampling Methods

[0052] 1.2 Required main reagents

[0053] Methanol

[0054] water

[0055] Chloroform

[0056] 1.3 The main instruments required

[0057] 4℃ Refrigerator: Haier Company

[0058] -80℃ ultra-low temperature refrigerator:

[0059] Analytical Balances:

[0060] Pipette:

[0061] Multi-sample tissue grinder (model Tissuelyser-192): Shanghai Jingxin Technology Co., Ltd.

[0062] Ultrasonic cleaner:

[0063] Ultracentrifuge:

[0064] Centrifugal Concentration Dryer:

[0065] Liquid Chromatography Mass Spectrometry

[0066] Liquid chromatography system (model Waters ACQUITY UPLC): American Waters Corporation

[0067] Mass Spectrometry System (Model AB Sciex Triple Qu

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Abstract

The invention discloses a detecting method for cholesterol metabolites in brain tissue. The detecting method comprises the steps that firstly, a cryopreserved brain tissue sample is accurately weighed; then a first reagent is added to conduct first-time extraction, a first extracting solution is collected, then a second reagent is added to conduct second-time extraction, a second extracting solution is collected, and the two-time extracting solutions are combined; and finally, after concentrating and volatilizing drying, a third reagent is added to conduct redissolving, and the extracting result is detected through a liquid chromatograph-mass spectrometer.

Description

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Claims

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Application Information

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Owner 上海博云生物科技有限公司
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