The invention provides preparation of a novel fluorescent imprinting sensor based on a magnetic
metal organic framework (MOF), and the novel fluorescent imprinting sensor is successfully used for detection of
hepatitis virus a (HAV). In the invention, light emitting MOF (MIL-101-NH2) is introduced to replace
quantum dots and organic dyes to serve as output signals. The light emitting MOF materialcan not only be used as the output signals, but also can be used as a carrier. Meanwhile, the
metal organic framework has a larger specific surface area, and thus can provide more imprinting sites, thereby improving the
response sensitivity of the sensor; due to the combination of
ferric oxide particles and the MIL-101-NH2 material, the process of preparing an imprinting
polymer is simpler and faster; and a
fluorescence analysis method has the advantages of high sensitivity, stronger selectivity, convenient operation and so on. Therefore, by combining the above several advantages, compared with other analysis methods or sensors, the
molecular imprinting fluorescent
chemical sensor constructed by the invention has good selectivity for template molecules and high detection sensitivity. The results show that the method is simple, convenient and fast, can be used for the qualitative and quantitative detection of HAV, and has important practical potential and significance in biosensing andvirus detection and prevention.