Method for detecting plasmodium ovale infection by using PCR technology

[0017] The design of specific primers: Example 1

[0018] Experimental material

[0019] Synthesis and nucleotide sequencing primers by Suzhou intellectualism Biotechnology Co., Ltd. completed.

[0020] 2. primer design

[0021] The GenBank database sequence encoding ovale (as shown in SEQ ID NO.1) and Plasmodium vivax and Plasmodium falciparum (P. falciparum: Gene ID: 3885775, Plasmodium vivax: GeneID: 5475443) in homologous sequence comparison, according to the difference sites designed sequence comparison of the PCR obtained ovale detection primer, designed to give the primers were synthesized by Suzhou intellectualism biotechnology company, specific detection of PCR primer sequences were as follows,

[0022] Primers were as follows:

[0023] SEQ ID NO2: ATGGTAGGGGCTAAAGGAGTG, SEQ ID NO.2;

[0024] SEQ ID NO3: CAATATGGTGAA AAAAACTGAGGAC, SEQ ID NO.3.

[0025] Example 2: PCR amplification of the target gene from a test sample

[0026] Experimental material

[0027] Genomic DNA extraction kit purchased from Tiangen Technology (Beijing) Company, Agarose TransGen powder available from the company, PCR instrument available from (Eppendorf).

[0028] 2. Extraction of genomic DNA to be detected in the serum

[0029] They were taken Plasmodium ovale, Plasmodium falciparum and Plasmodium vivax six patients in each of the DNA samples extracted using Tiangen Technology (Beijing)'s blood / cell / tissue genomic DNA extraction kit, according to the method description parasitemia level of genomic DNA genomic DNA, extracted, and the extracted DNA samples provided at -20 ℃ refrigerator for use, the samples were as shown in table 1.

[0030] 3.PCR amplified specific bands

[0031] Use Suzhou intellectualism biotechnology companies synthesized primers specific to the genomic DNA samples extracted from amplified verified, the PCR instrument using