6 results about "Phosphorylation" patented technology

The invention relates to anti-tumor activity polypeptide and application thereof.The polypeptide has anti-tumor activity due to the fact that the polypeptide has one of the following amino acid sequences of RMKKRYPTTFVMVV and IGLKRTPMGIV, wherein Y or the second T counting from left to right in RMKKRYPTTFVMVV is modified through phosphorylation, and T in IGLKRTPMGIV is modified through phosphorylation.The polypeptide can be used for preparing an anti-tumor drug.The sequence of the polypeptide is short, and large-scale production is easily achieved; remarkable anti-tumor activity is displayed at a low dosage.The polypeptide has no toxicity to normal tissue cells and has wide application prospects.

The invention discloses a stress phosphorylation antibody aiming at a human Tudor-SN protein T103 site and a preparation method thereof. The preparation method adopts a multifunctional human Tudor-SN protein as a research object and comprises the following steps of determining if threonine at a 103 site of a human Tudor-SN protein under external environment oxidation stress is subjected to phosphorylation modification by near-infrared double-color laser imaging and mass spectrometry, synthesizing a T103 phosphorylation site-containing semiantigen polypeptide according to the secondary structure prediction result, coupling the T103 phosphorylation site-containing semiantigen polypeptide and a carrier protein (KLH) into a complete antigen, carrying out immunization on SPF-level New Zealand white rabbit by the complete antigen combined with an adjuvant four times and collecting, purifying and identifying a T103 specific stress phosphorylation polyclonal antibody. In practical application, Tudor-SN protein epigenetic phosphorylation modification in different stress environments can be detected, the effect mechanism of the Tudor-SN protein epigenetic phosphorylation modification in cell stress tumor propagation and migration can be discussed and a latent action target point for clinical tumor disease diagnosis and treatment is provided.

The invention discloses application of sucrose phosphorylase in preparation of 2-O-alpha-D-glucosyl-L-ascorbic acid. According to the application, a reaction system is formed by taking fermentation liquor, obtained through fermenting sucrose phosphorylase gene containing engineering bacteria, or broken liquor, obtained through resuspending wet thalli obtained after centrifuging the fermentation liquor with a buffer solution, as a catalyst, taking L-ascorbic acid as a substrate, taking sucrose as a cosubstrate and taking a buffer solution with a pH of 4.8 to 6.0 as a reaction medium, and a reaction is carried out at the temperature of 30 DEG C to 55 DEG C, thereby obtaining AA-2G. According to the application, the AA-2G is efficiently produced by employing the sucrose phosphorylase, the yield is 3.47g/L/h and is increased by 34.5% compared with that employing the existing reported B. longum derived sucrose phosphorylase, and the maximum concentration of the AA-2G can reach 178g/L; and recombinant bacillus subtilis can efficiently secrete and express the sucrose phosphorylase, and the fermentation liquor is directly applied to preparation of food-grade AA-2G.