Cell-penetrating peptide

a cell-penetrating peptide and peptide technology, applied in the field of cell-penetrating peptides, can solve the problems of low cell penetration efficiency, method limitation, and inability to migrate into the cell of most peptides, and achieve the effects of maintaining the functions of the cell and the material, minimizing side effects, and stable structur

Active Publication Date: 2019-05-02
NEOREGEN BIOTECH
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Benefits of technology

The inventive technology described in this patented patents allows for efficient transferring of materials inside specific areas called cytoskeletal spaces that are difficult to access through other methods like injection. This makes these technologies useful across different medical settings where they may benefit from their ability to target certain substances specifically towards particular tissues/cells.

Problems solved by technology

This patented method involves designing small molecules called Cpppets(CMPs)) that can enter cells effectively when crossing them' boundaries during passage across biological barriers. These small molecular structures allow certain substances to cross from inside to outside the body quickly enough despite its size. They provide efficient ways to introduce agents within living systems where they cannot reach all parts of the system because they lack specific characteristics needed for targeted entry. Additionally, the new CPP should exhibit good cell pension ability and transferring material throughout blood vessels and lymbriae without causing harmful reactions at least partially.

Method used

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[0066](1) Synthesis of Cell-Penetrating Peptide having Greatly Higher Cell Penetration Efficiency even in an Environment including Serum or Sera And Measurement of Cell Penetration Efficiency

[0067]In order to confirm the cell penetration efficiency of the cell-penetrating peptide according to the present invention, an experiment was conducted by including Tat-PTD (SEQ ID NO: 12), which is a well-known cell-penetrating peptide, as a control group.

[0068]In order to confirm the cell penetration efficiency of each cell-penetrating peptide, fluorescein isothiocyanate (FITC, fluorescent material), which fluoresces each peptide, was adhered to each peptide, then penetration efficiencies thereof were examined.

[0069]When measuring the cell penetration efficiency, in order to remove each cell-penetrating peptide which may be adhered to the cell membrane surface, a Heparin & Trypsin washing method used in the Kaplan group was applied thereto [Kaplan I M, Wadia J S, Dowdy S F (2005) Cationic TAT p

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Abstract

A cell-penetrating peptide has remarkably excellent cell penetration efficiency, and is capable of penetrating 100% into all cells within 1 hour even in an environment including serum or sera, as well as a stable structure. Therefore, it is possible to move a cargo into the cell while maintaining the function of the cargo without damaging the same.

Description

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Claims

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Application Information

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Owner NEOREGEN BIOTECH
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