Cell preservation solution and application thereof in preservation of umbilical cord blood NK cells

一种保存液、细胞的技术，应用在细胞培养领域，能够解决单个核细胞分离工作限制等问题

Active Publication Date: 2020-12-11
GUANGDONG XIANGXUE STEM CELL REGENERATIVE MEDICINE TECH CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to maintain the activity of NK cells in fresh umbilical cord blood, heparin sodium is often used for collection at present, and it must be processed within 4 hours after collection. Some studies have also shown that: when the normal ratio of umbilical cord blood to anticoagulant is maintained, clinical fresh Enrichment of NK by Ficoll separation within 7 hours of cord blood storage at 37 °C can achieve a good enrichment effect, which has caused certain limitations to the collection of cord blood and the isolation of mononuclear cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Preparation of cell preservation solution: first dissolve 3 g of trisodium citrate, 0.03 g of citric acid, 3 g of glucose, 0.6 g of hydroxyethyl starch, and 2 g of human serum albumin in 5 ml of water for injection, and then dilute to 10 ml with water for injection. Store at 4°C.

[0042] 2. Collection and preservation of human fresh cord blood: put 100mL cord blood (collected in a sodium heparin collection bag containing 10mL 20IU / ml) at 1-6°C while avoiding X-rays.

[0043] 3. Packing of human fresh umbilical cord blood: take the umbilical cord blood in the 30mL collection bag and put it in a 50mL centrifuge tube, add 3mL of cell preservation solution, and store for 72h.

[0044] 4. Flow cytometry detection of human umbilical cord blood: In a safety cabinet, extract 1ml of umbilical cord blood stored for 72 hours, and use a BD flow cytometer to detect the content of NK.

[0045] 5. Separation of human cord blood mononuclear cells: Using density gradient centrifuga...

Embodiment 2~ Embodiment 5

[0051] Embodiment 2~5 steps 1~7 are with embodiment 1. 100mL of umbilical cord blood was collected in Examples 2-5.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of cell culture, in particular to a cell preservation solution and application thereof in preservation of umbilical cord blood NK cells. The invention discloses a cell preserving fluid which comprises trisodium citrate, citric acid, glucose, hydroxyethyl starch, human serum albumin and water. Trisodium citrate, citric acid and glucose in the cell preserving fluid cooperate with hydroxyethyl starch and human serum albumin, the preservation time of umbilical cord blood is effectively prolonged to 72 h, it is guaranteed that the survival rate and number of umbilical cord blood NK cells are basically unchanged before preservation, and the NK cells with the normal multiple and number can be obtained through separation and multiplication culture.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell preservation solution and its application in preserving umbilical cord blood NK cells. Background technique [0002] Natural killer cells (NK) are important immune cells in the human blood system. As we all know, the first line of defense of the body's defense system is NK cells, which can secrete different cytokines and various chemokines in the early stage of the immune response to regulate the body's acquired immune response, and play a major role in killing tumors in the natural immune system. Effector cells are indispensable effector cells for the body to exert immune effects. [0003] Umbilical cord blood is rich in sources, and cord blood has become one of the main sources of NK cells at present. In order to maintain the activity of NK cells in fresh umbilical cord blood, heparin sodium is often used for collection at present, and it must be processed within ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 张玲洁陈爱华张林艳
Owner GUANGDONG XIANGXUE STEM CELL REGENERATIVE MEDICINE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap