57 results about "Peptide" patented technology

Isolated and purified peptides and variants thereof, useful to prevent or treat antibody-mediated diseases, or indications caused by an undesirable antibody response to a given antigen, are provided. Also provided are peptides and methods useful to prevent or treat indications associated with the use of viral vectors in gene replacement therapy. Further, a method to inhibit or prevent aberrant immune responses to exogenous, non-infectious antigen is provided.

The invention concerns multimers developed from recombinant proteins analogues of MHC class I.

The disclosure relates to a method of generating a diverse set of variants to screen improved and novel properties within the variant population, a system for creating the diverse set of variants, and the variant peptides.

A human fusion antibody for preventing and treating senile dementia features that the beta-amyloid peptide and the recognition and bind regions of the fibres generated by it, the connecting peptide of 2-4 amino acids, and the human antibody Fc segment recognized by human immunocells are sequentially contained by its terminals from N to C. The fusion gene for coding the said antibody is also disclosed.

Standard reference materials and related methods are described for quality testing and calibration of instruments used for the qualitative and quantitative determination of hemoglobin and glycated hemoglobin as well as other analytes of interest in animal tissue samples. The reference solutions of the disclosure utilize a synthetic cruor in combination with an Hb peptide chain.

The invention provides an enhancement fodder for improving pig's food consumption, which comprises the following components: flavoring peptides 80-120ppm, sea product extract 60-100ppm, organic acid 1000-2000ppm, natural vegetable oil 30-70ppm, I plus G 10-50ppm, 50% vitamin E powder 100-200ppm, pure vitamin C powder 100-300ppm. The invention also discloses the preparing process.

The invention discloses a synthesizing method of sermaglutide. The synthesizing method comprises the following steps of step 1, preparing Fmoc-Gly-Wang resin; step 2, using the Fmoc-Gly-Wang resin, protective amino acids, and octadecandioic acid (OtBu)-gamma-Glu-OtBu-PEG-PEG-OH as the raw materials to prepare full-protective peptide resin which contains Depsipeptide Units; step 3, performing TFA (trifluoroacetic acid) cracking on the peptide resin, so as to obtain crude peptide; step 4, dissolving the crude peptide obtained in step 3, adjusting the pH (potential of hydrogen) value, and converting ester bonds into amide bonds to react, so as to obtain a crude product of the sermaglutide. The synthesizing method has the advantages that by introducing the Depsipeptide Units, the problem of difficulty in sequential synthesizing of the sermaglutide is solved, and the target product is obtained by the reaction of converting the ester bonds into the amide bonds; the purity and yield of the crude peptide are improved, and the synthesizing method is suitable for downstream purifying, and is suitable for industrialized production.

A method for inhibiting dipeptidyl-peptidase IV is provided and includes administering a peptide to the dipeptidyl-peptidase IV, in which the peptide is isolated from a gelatin hydrolysate produced by enzymatic digestion with alcalase (ALA), bromelain (BRO), and flavourzyme (FLA).

The present invention relates generally to microtiter plate format devices and methods for separating molecules having different net charges. The devices and methods of the invention are particularly suited for use in high-throughput screening to monitor enzymatic reactions which result in a product having an altered net charge. For example, the systems and methods disclosed herein may be used to detect the activity of phosphatases, proteases and kinases on various peptidic substrates under various conditions.

The invention discloses a preparation method of oryzenin antioxidant peptide, comprising the following steps: (1) screening rice slag with a mesh of 50-200 meshes, and adding water according to the material and water weight ratio of 1:6-12 to be dipped for 10-15 minutes; (2) pouring rice slag feed liquid into a colloid mill to be ground for more than or equal to 2 minutes, and grinding rice slag particles in the feed liquid; (3) adjusting the pH of milled and homogenized feed liquid to 8.0-9.5, adding phosphate buffer, adding alkali protease 200U/g-600U/g rice slag, stirring and carrying out enzymolysis for 1-3 hours at the temperature of 40-65 DEG C, and controlling hydrolyzing degree to be 10-15wt%; (4) killing enzyme and carrying out centrifugal separation; (5) vacuum concentrating, freezing and drying. When adopted to prepare oryzenin antioxidant peptide, the invention has wide raw material resource and low manufacturing cost, the obtained oryzenin antioxidant peptide has high antioxidant activity and high edible safety.

Compositions are provided comprising a family of peptides having binding specificity for bone, and their use to produce coating compositions. The coating compositions are used to deliver a pharmaceutically active agent to bone, and are used in methods related to bone implants, bone repair, and bone-related diseases.

A process for preparing the active embryonic peptide with coproduct of corn oil from the embryo bud of corn at the same time includes such steps as wet and thermal pretreating, grinding by colloid mill, multi-stage zymolysis, centrifugal separation to obtain fibres, corn oil and milk, concentrating the milk by nanometre filtering membrane, further concentrating, homogenizing, emulsifying, drying and granulating.

The invention discloses tomato egg coarse cereal noodles. The noodles comprise the following materials in part by mass: 200 to 250 parts of tomatoes, 350 to 450 parts of flour, 30 to 50 parts of corn peptide, 100 to 120 parts of eggs and 0.4 to 0.8 part of salt. The preparation method comprises the following steps of: mixing 350 to 400 mass parts of flour, 30 to 50 mass parts of corn peptide and 0.4 to 0.8 mass part of salt, the tomatoes which are washed, peeled and capped and crushed into sauce, and 100 to 200 mass parts of eggs which are beaten to be bubbly, uniformly stirring to form pulpy noodle materials, curing at room temperature for 15 to 20min, pressing the cured dough into wet noodles by using a noodle molding machine, drying, and packing to obtain a finished product. The tomato egg coarse cereal noodles are added with the corn peptide, the tomatoes and the eggs, and have higher nutrient value and better taste than the conventional noodles.

Modified fusion proteins of transferrin and therapeutic proteins or peptides, preferably antibody variable regions, with increased serum half-life or serum stability are disclosed. Preferred fusion proteins include those modified so that the transferrin moiety exhibits no or reduced glycosylation, binding to iron and/or binding to the transferrin receptor.

The present invention relates to trifunctional crosslinking reagents carrying (i) a ligand-reactive group for conjugation to a ligand of interest having at least one binding site on a target glycoprotein receptor, (ii) an optionally protected aromatic hydrazine group for the capturing of oxidized receptor-glycopeptides and (iii) an affinity group for the detection, isolation and purification of captured glycopeptides, their methods of production, as well as their use in methods for detecting, identifying and characterizing interactions between ligands and their corresponding glycoprotein target receptors on living cells and in biological fluids.

The invention belongs to the technical field of food biology, and discloses a soybean peptide with high ACE inhibitory activity, and a preparation method and application of the soybean peptide. The soybean peptide has the high ACE inhibitory activity, and the preparation method comprises the following steps of adding alkaline protease to soybean protein isolate for enzymolysis to obtain an enzymolysis product; separating the enzymolysis product with a membrane ultrafiltration method to obtain soybean peptide powder I; further purifying the soybean peptide powder I with an ethanol fractionationmethod to obtain soybean peptide powder II; and further separating and purifying the soybean peptide powder II by using active carbon adsorption chromatography to obtain the soybean peptide with thehigh ACE inhibitory activity. The soybean peptide has the excellent ACE inhibitory activity and the good blood pressure reducing effect, can be applied to the field of food and health products, and the preparation method is simple and controllable and is suitable for mass production in factories.

The present invention discloses diamondback moth cecropin 3, a preparation method and an application thereof, wherein the diamondback moth cecropin 3 gene has a nucleotide sequence represented by SEQIDNO:1-2 and has a corresponding amino acid sequence represented by SEQIDNO:3, and sequence analysis results show that the mature peptide of the diamondback moth cecropin 3 has an amino acid sequence represented by SEQIDNO:4. According to the present invention, the diamondback moth cecropin 3 gene or the mature peptide sequence thereof is subjected to prokaryotic expression to obtain the diamondback moth cecropin 3, and the obtained diamondback moth cecropin 3 provides strong inhibition effects for gram-negative bacteria and gram-positive bacteria, provides strong inhibition effects for pathogenic fungi, especially provides strong inhibition effects for a lot of pathogenic fungi of melons and fruits, and is expected to be a novel peptide antibiotic.

The invention relates to sturgeon meat quality improving bait and a preparation method thereof. The bait is prepared from components including, by weight, 10-20 parts of field snail powder, 20-30 parts of fish powder, 10-20 parts of earthworm powder, 8-12 parts of selenium-enriched yeast autolyzed powder, 5-10 parts of rice bran peptide, 0.5-1 part of multi-vitamins, 8-12 parts o and an immunopotentiator; the immunopotentiator is prepared from traditional Chinese medicine raw materials including, by weight, 6-10 parts of common andrographis herb, 6-10 parts of fructus forsythiae, 6-10 parts of radix bupleuri, 6-10 parts of folium artemisiae argyi, 6-10 parts of codonopsis pilosula, 6-10 parts of astragalus membranaceus, 6-10 parts of honeysuckle, 6-10 parts of medlar, 6-10 parts of radix isatidis and 6-10 parts of houttuynia cordata. The sturgeon meat quality improving bait is reasonable in compatibility, balanced in nutrition, low in processing cost, good in tastiness and food calling, high in sturgeon utilization, few in metabolic waste, obviously high in immunity of sturgeons, free from drug residue and drug resistance, good in safety and capable of effectively improving the quality of the sturgeons.

The present invention is an inhibitor of the trypsin-like β2/β2i sites of the proteasome. The inhibitor is characterized as being a peptide-based epoxyketone or vinyl sulfone that contains an arginine or 4-aminomethylene-L-phenylalanine at the C-terminus (i.e., at the P1 position). Methods for using the inhibitor to inhibit the activity of the β2/β2i site of a proteasome and treat a proteasome-mediated disease or condition are also described.

[Problem] To make a color of a processed food containing anthocyanin pigment into a vibrant color. In this respect, using a common foodstuff that is cheap and highly safe, without adding a coloring agent, and not imparting an incongruous flavor to the food is preferred.

[Means for Solving the Problem] To an anthocyanin pigment or a common processed food containing anthocyanin pigment is added, in an appropriate amount, a yeast extract containing 5 wt % or more of a peptide, 5 wt % or more of RNA, 4 wt % or less of free amino acid, and preferably further containing 15 wt % or more of dietary fiber.

The invention relates to a sheep placenta peptide extraction method, which comprises steps of cleaning, digesting, discharging soup, hydrolyzing, filtering, concentrating and spray-drying. With the application of the extraction method disclosed by the invention, problems on the effective absorption and utilization of macromolecular protein in human body are effectively solved, and micromolecular transformation and extraction of the protein are achieved. Rich tissue protein in sheep placenta is transformed into micromolecular peptide through directional biological enzymolysis; the protein, which is derived from an animal body, is quite close to human body in structure and function, and the protein, which has a micromolecular structure, can be directly absorbed by intestines without digestion and can rapidly participate in normal metabolism and regulation of the human body; therefore, the protein, in pharmacology, is higher in biochemical mechanism reasonability and specific treatment effectiveness. The sheep placenta peptide has the characteristics of being strongly targeted, free from toxic and side effects, significant in curative effect, high in nutritive value, easy to to be absorbed by human body and the like.

Encapsulated bitter peptides, methods of encapsulating bitter peptides, and nutritional compositions including encapsulated bitter peptides are provided. The bitter peptides can be encapsulated in a hydrophobic matrix, such as an organogel, a solid lipid nanoparticle, a liposome or combinations thereof. The encapsulated bitter peptides can be bioavailable, can be selectively encapsulated such that peptides needed for emulsion stabilization are not encapsulated and can maintain encapsulation during further processing such as heat treatment and homogenization.

A protein product extracted from apathogen bacilli is described. It comprises proteins, peptides and other molecules, obtained from cellular lysis of at least one of the sporulated and thermal resistant, natural or modified, bacilli strains selected from: a first group composed of strains of B. Licheniformis, B. Circulans 2, B. Pumilus, B. Macerans, B. Amilolicuofaciens; a second group composed of strains of B. Cereus 1, B. Cereus 2, B. Lentus 1, B. Lentus 2; a third group composed of strains of B. Subtilis; a fourth group composed of strains of B. Mesentericus. Also is described a process for the preparation of the protein product, compositions containing it and its use in the preparation of medicaments for the treatment of diseases related to immunedeficiency syndrome, auto-immunity, neoplasic processes, degenerative articular diseases and inflammatory intestinal diseases.

The invention relates to a pharmaceutical composition for treating cerebral apoplexy and acute cerebral infarction. The pharmaceutical composition takes active peptide Apelin as an active ingredient,and the structure of the active peptide Apelin is 2-(4-chlorphenyl)-2, 2-difluoroacetic acid-QRPRLSHKGPMPF. The pharmaceutical composition disclosed by the invention has a bidirectional treatment effect on cerebral arterial thrombosis, and can relieve neurological dysfunction of the cerebral arterial thrombosis and reduces the cerebral infarction volume.

The invention provides to a method for cloning and expressing recombinant chicken interferon (IFN)-gamma genes and detecting the activity of the recombinant chicken IFN-gamma genes. The method comprises the following steps of: augmenting ChIFN-gamma gene segments without the signal peptide by taking recombinant plasmids PMD-18T-preIFN-gamma with signal peptide as a template; subcloning the product after being sequenced to an expression vector pProEXTMHTa; converting the expression vector pProEXTMHTa-ChIFN-gamma into an expression host E.coli DH5alpha; and optimizing the best induction condition. Due to the method, a foundation is formed for researching and developing a novel anti-virus interferon preparation. When purified recombinant ChIFN-gamma fusion protein is used for performing immunization on broilers, the result shows that: under the heat stress condition of the ChIFN-gamma fusion protein, the HSP-70 expression quantity of different organs can be influenced; the recombinant ChIFN-gamma fusion protein delays the generation of the HSP-70; and the negative influence caused by the heat stress can be relieved.