Method for high-throughput screening recombinant trichoderma reesei for efficiently expressing foreign protein

The invention discloses a method for high-throughput screening recombinant trichoderma reesei for efficiently expressing a foreign protein. The method comprises the steps as follows: comparing the red fluorescence intensity of recombinant trichoderma reesei for expressing the foreign protein identified by a flow cytometry meter(FCM); identifying the expressing ability of the foreign protein according to the red fluorescence intensity; and obtaining a result that the expressing ability of the foreign protein of the recombinant trichoderma reesei for expressing the foreign protein having higher red fluorescence intensity is strong. The gene of the foreign protein is led into the trichoderma reesei in a mode of a fusion gene, the fusion gene is formed by connecting a red fluorescence protein gene with the foreign protein gene through a connecting peptide 2A sequence; the foreign protein gene is positioned in the upstream or the downstream of the red fluorescence protein gene; a signal peptide sequence is formed at the 5' end of the foreign protein gene; and connecting peptides 2A are shown in 290th to 306th positions of the 8th sequence in the coding sequence list of the connecting peptide 2A sequence.