4 results about "Glycosylation" patented technology

The current invention relates to the development and methods of use of a recombinant agonistic antibody anti-human CD137, and glycosylation variants thereof. These antibodies act as an anti-cancer agents and/or immune modulators that are effective in shrinking solid tumors or other cancerous indications and preventing their recurrence. The types of cancer for which the contemplated antibody is effective in treating also include leukemia and lymphoma. In a preferred imbodiment the recombinant antibodies of the current invention were produced in and purified from the milk of transgenic animals.

The invention belongs to the field of protein analysis, and relates to a new method for enrichment of a glycosylated peptide through a magnetic nanometer material modified by aminoxy, in particular to a method for solid-phase enrichment and mass spectrographic analysis of a glycosylated peptide fragment. The method comprises the following steps: firstly, using sodium periodate NaIO4 to oxidize a hydroxyl group on a glycopeptide to change the hydroxyl group to an aldehyde group; secondly, placing the magnetic nanometer material Fe3O4@ONH2 modified by aminoxy to an oxidized glycopeptide solution, and fixing the glycopeptide to the magnetic nanometer material through reaction of an aminoxy group and the aldehyde group obtained after a sugar chain in the glycopeptides is oxidized; thirdly, cleaning to remove non-glycopeptide which does not react with the nanometer material; finally, dissociating the captured glycopeptide from the material by use of sugar chain enzyme, and realizing mass spectrographic analysis of the glycosylated peptide. The method is simple in step, convenient to operate, high in speed and efficient, and can realize highly-sensitive and highly-selective mass spectrometric analysis of the glycosylated peptide.

Transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.

The invention discloses a jade butterfly nut potato mash which comprises the following raw materials in parts by weight: 20-22 parts of mung bean, 18-20 parts of pistachio nuts, 5-6 parts of cookies, 8-9 parts of soybean milk, 1.3-2.3 parts of jade butterfly, 1.5-2.1 parts of mountain mint, 1.3-2 parts of pearl fruit, 200-230 parts of potato, 100-120 parts of streaky pork, 8-10 parts of salt, 3-4 parts of chilli powder, 2-3 parts of pepper powder, 2-3 parts of sodium carboxymethylcellulose, 1-1.2mol/l NaOH, 100-150 parts of selenium-rich peanuts, 0.1-0.15 parts of succinic anhydride and a defined amount of phosphate buffer and petroleum ether. In the jade butterfly nut potato mash, sodium carboxymethylcellulose and peanut protein isolate have glycosylation reaction, so as to form a covalent compound capable of maintaining the excellent emulsification property of food at high temperature, prolonging polypeptide chain, enhancing steric effect and enhancing the heat stability of the compound.