33results about "Foreign genetic material cells" patented technology

The present invention provides a monoclonal anti-idiotype antibody 11D10 that elicits an immune response against a specific epitope of a high molecular weight mucin of human milk fat globule (HMFG) and a hybridoma that produces 11D10. The hybridoma that produces 11D10 was selected by specific procedures. 11D10 induces an immunological response to HMFG in nice, rabbits, monkeys and patients with advanced HMFG-associated tumors. This invention provides compositions derived from polynucleotide sequences encoding the variable light and/or variable heavy regions of monoclonal anti-idiotype antibody 11D10, as well as polypeptides encoded thereby. The invention also provides compositions which can be used in the detection or treatment of HMFG-associated tumors.

The present invention provides methods, compositions, and kits for the treatment of disorders associated with overactivation of FGFR3, such as achondroplasia; bone or cartilage disorders; or vascular smooth muscle disorders, or for the elongation of bone. In some embodiments, the present invention provides polypeptides having a natriuretic peptide fused to an Fc domain of an immunoglobulin. Such polypeptides can be administered to subjects, e.g., subcutaneously, to treat a disorder associated with overactivation of FGFR3, a bone or cartilage disorder, or a vascular smooth muscle disorder, or to elongate bone. The invention also features nucleic acid molecules encoding such polypeptides and the use of the nucleic acid molecules for treating disorders associated with overactivation of FGFR3, bone or cartilage disorders, or vascular smooth muscle disorders, or for elongating bone.

The present invention provides data to demonstrate that the re-fusion, of a mammalian karyoplast to an enucleated in vivo ovulated cocyte, following an unsuccessful initial simultaneous electrical fusion and activation event offers an additional alternative and improvement in the creation of activated and fused nuclear transfer-capable embryos for the production of live off-spring in various mammalian non-human species including goats, pigs, rodents, primates, rabbits and cattle. Additionally, multiple electrical pulses offers an alternative and more efficient activation method in a simultaneous fusion and activation methodology for viable offspring production in a animal nuclear transfer program.

Novel MCP-1 splice variant polypeptides and polynucleotides encoding same are provided. Also provided are pharmaceutical compositions comprising the splice variant polypeptides and polynucleotides, vectors and host cells comprising same. The compositions of the present invention are useful to treat various MCP-1 related disorders as well as for diagnosing, determining predisposition and/or prognosis of various disorders.

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

The invention provides a reporter gene assay method by which the sex hormone-like activity inherent in a test substance can be accurately assayed excluding the effects caused by a decrease in cell activity (protein expressing capacity). The assay method is a reporter gene assay method using luciferase-expressing cells which comprises further tranferring a GFP gene into the luciferase-expressing cells, measuring the luciferase expression level and GFP expression level, and making a judgment about the thus-measured luciferase expression level using the decrease in GFP expression level as an indication of the decrease in cell activity.

The present invention relates to a Lactuca sativa seed of the cos type designated 41-137 RZ, which exhibits a combination of traits including resistance against downy mildew (Bremia lactucae) races B1:1 to B1:28 and CA-I, CA-IIA, CA-IIB, CA-IV, CA-V, CA-VI, and CA-VIII, currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, root aphid (Pemphigus bursarius), and lettuce mosaic virus (LMV) race LMV:1. The present invention also relates to a Lactuca sativa plant produced by growing the 41-137 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-137 RZ.

The present invention provides transgenic mice deficient in corticotropin releasing factor receptor 2 (CRFR2). Mice deficient for CRFR1 exhibit decreased anxiety-like behavior and a decreased stress response. In contrast, CRFR2 null mutant mice are hypersensitive to stress and display increased anxiety-like behavior. These mice are useful for the study of anxiety, depression, and the physiology of the HPA axis. CRFR2 null mutant mice also exhibit increased angiogenesis in all tissues examined. Thus, CRFR2 antagonists may be used to stimulate angiogenesis for the treatment of various conditions. In contrast, CRFR2 agonists may be used to inhibit angiogenesis. A combination of urocortin and bFGF was observed to stimulate rapid hair growth.

The invention discloses a method for culturing dwarfed lawn grass by using a fusion protein. The fusion protein provided by the invention contains trehalose phosphate synthase containing chloroplast transit peptide, wherein the chloroplast transit peptide is positioned at the amino terminal of the fusion protein; the trehalose phosphate synthase is positioned at the carboxyl terminal of the chloroplast transit peptide. The fusion protein also contains trehalose phosphatase, wherein the trehalose phosphatase is positioned at the carboxyl terminal of the trehalose phosphate synthase. The dwarfed lawn grass can be obtained by transferring the coding genes of the fusion protein into a plant. The fusion protein and the coding genes can be used for culturing the new and beautiful dwarfed lawn grass requiring less pruning.

A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.

The invention belongs to the technical field of biological preparation and in particular relates to an RXRalpha (Homo Sapiens Retinoid X Receptor alpha) gene knockout cell system with stable and low expression of an RXRalpha protein and a preparation method of the RXRalpha gene knockout cell system. The cell system is an RXRalpha gene knockout cell system; the knockout base number is an integral multiple of a number except 3; RXRalpha protein expression in a cell is remarkably reduced, the genotype is stable and can be passed to next generations, the stability of the RXRalpha low-expression cell system is greatly improved, and stable generation passage and continuous culture of the cell system can be facilitated. The invention provides the preparation method of the cell system. The preparation method comprises the following steps: according to RXRalpha gene sequence information, designing a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) knockout gRNA (Guide Ribonucleic Acid) sequence, establishing a gRNA expression vector, and carrying out in-vitro cell level detection on gRNA shearing activity; by using a nuclear transferring method, carrying out cotransfectionon an immortalized cell of human neuroblastoma, that is, an SK-N-SH cell, by using cas9 and the gRNA expression vector, carrying out G418 medicine screening so as to obtain stable cell cloning, carrying out a PCR (Polymerase Chain Reaction) and gene sequencing to identify that the cell is cell cloning of which the RXRalpha gene is knocked out for an integral multiple of a number except 3, therebyobtaining the RXRalpha gene knockout cell system with stable and low expression of the RXRalpha protein.

The invention relates to the field and particularly relates to an expression vector for an animal cell. The expression vector sequentially contains a promoter with strong expression inductivity, a multiple cloning site for gene integration, a ribosome entry site sequence, a dihydrofolate reductase gene, a SV40 early stage promoter gene without an enhanser, a neo gene and an anti-drug gene from upstream. The invention further provides a preparation method of the expression carrier, a recombinant carrier and application. According to the expression carrier provided by the invention, expression of the neo gene is weakened by using the promoter without the promoter; a dhfr gene as a co-amplification gene is located at the downstream of the ribosome entry site IRES, so that the translation efficiency of the dhfr gene is reduced; the expression quantity of a target protein is increased by combining G418 pressurized screening and pressure screening of the dihydrofolate reductase gene.

Process for obtaining genetically modified plants, especially woody plants, especially plants of the genus Eucalyptus and Pinus, comprising transforming target plant material with a desired genetic construct and transforming transformed plant material using an adventitious shoot system The material is recycled. The present method has high transformation efficiencies and significantly reduces the duration of transformation and regeneration protocols. Stem nodes of target plants were transformed by Agrobacterium-mediated technology, and adventitious shoots were regenerated from stem nodes infected with Agrobacterium. There are also preferred culture media, including selection media, and improved plant culture techniques.

The invention discloses a fusion protein VT-GL-B3, a coding gene thereof and applications thereof. The fusion protein is (a) or (b) or (c) as follows: (a) a protein formed by amino acid residues from the 21 site to the 468 site in the sequence 1 from the N end; (b) a protein formed by the amino acid sequence shown as the sequence 1; and (c) a derived protein obtained by subjecting the (a) or the (b) to substitution and/or deletion and/or addition of one or more of amino acid residues and related to angiogenesis. The invention also provides the applications of the fusion protein in preparation of medicines. The function of the medicines is at least one of the (I) to (V) as follows: (I) inhibition of vascular endothelial cell proliferation and/or migration; (II) inhibition of inhibit angiogenesis; (III) inhibition of tumor growth; (IV) treatment of cancer; and (V) treatment of angiogenesis-related diseases. The fusion protein, the coding gene thereof and the applications thereof have significant value for treatment of cancer and other angiogenesis-related diseases.

The object of the present invention is to obtain a novel transposon-like element specifically present in the genome of rye and the like. According to the present invention, a DNA sequence of a transposon-like element Revolver comprising 3,041 nucleotide pairs, and DNA sequences of structural mutants thereof were provided. The DNA sequence of the transposon-like element Revolver, the DNA sequences of genes having transcriptional activity encoded by Revolver, and the DNA sequences of structural mutants thereof can be utilized for detection of a genome, development of DNA markers, identification of chromosomes, a probe for study on evolution, an entry point of PCR and the like, in useful resource plants of Poaceae.

The invention relates to the technical field of cell culture and provides immune cell CAR-T culture equipment with a high cell survival rate and a culture method. The immune cell CAR-T culture equipment comprises an equipment outer frame, a culture rack and an adjusting rotating valve, wherein the equipment outer frame comprises an observation window, the culture rack comprises a culture inner chamber, the culture rack is arranged at the inner side of the equipment outer frame, a culture bin used for culturing cells is arranged at the inner side of the culture inner chamber, the observation window is arranged at the top end of a reaction tube, the reaction tube used for sensing an internal carbon dioxide concentration is movably connected to the inner side of the culture bin, and a transfer tube used for receiving nutrients is movably connected to the side, away from the reaction tube, of the culture bin. An auxiliary block drives an auxiliary clamping block to move towards the outer side through a rotating rod column, the auxiliary clamping block drives a sliding block to move towards the outer side, and a worker rapidly checks specific conditions of internal cells through the observation window, so that the survival rate of the cells can be observed at any time, and accidental death of the cells is avoided.

The invention provides a recombinant cell strain HSCT6-rADHI as well as a construction method and application of the recombinant cell strain HSCT6-rADHI. The recombinant cell strain HSCT6-rADHI is obtained by introducing an ethanol dehydrogenase I gene into a receptor cell strain HSC-T6, and it is found that after high expression of ADHI by HSC, proliferation, invasion and migration capacities of cells can be obviously affected. The recombinant cell strain HSCT6-rADHI can efficiently express ADHI, can be used as a basic test material and a model cell strain, can be used for exploring the occurrence mechanism of hepatic fibrosis, searching for new alleviation measures for hepatic fibrosis, reversing or blocking the hepatic fibrosis process and searching for susceptible factors of hepatic fibrosis, is beneficial to prevention and further targeted therapy of hepatic fibrosis, and has a broad application prospect. The significance is great.

The invention relates to the technical field of biology, in particular to new application of snoRA7A and a method for enhancing the proliferative capacity of hUCMSCs under an invitro culture condition. According to the method for enhancing the proliferative capacity of the hUCMSCs, expression plasmids of the snoRA7A are successfully constructed, the plasmids are used for transfecting the hUCMSCs, hUCMSCs achieving high expression of the snoRA7A are obtained, and compared with original hUCMSCs, the obtained hUCMSCs are higher in proliferative capacity and better in pluripotency. A larger number of cells with a better functional state can be obtained under the invitro culture condition, and the problem that biological activity of the proliferative capacity and the like of the hUCMSCs is decreased along with increase of the passage number in the invitro culture process is solved. By means of the method for enhancing the proliferative capacity of the hUCMSCs, a new idea is provided for maintaining and enhancing of the cell proliferative capacity of the hUCMSCs in invitro culture and passage process, and a wider prospect is provided for clinical application of the hUCMSCs.

The invention discloses a soybean powdery mildew resistance gene GmRmd1 encoding protein and application thereof, and belongs to the technical field of gene engineering. The invention provides an application of any one of the following substances in improving powdery mildew resistance of soybeans: 1) a GmRmd1 gene DNA molecule; 2) a GmRmd1 encoding protein; 3) a recombinant plasmid, an expression cassette, a transgenic cell or a recombinant bacterium containing a GmRmd1 gene DNA molecule; the amino acid sequence of the GmRmd1 gene is shown as a sequence 1 in a sequence table. The GmRmd1 gene disclosed by the invention is expressed in soybean varieties susceptible to soybean powdery mildew, so that transgenic soybeans can be endowed with a powdery mildew resistant function; the resistance of powdery mildew can be lost by mutating the GmRmd1 gene in the soybean powdery mildew resistant variety. The gene disclosed by the invention can be introduced into a soybean powdery mildew susceptible variety as a target gene to endow the soybean powdery mildew susceptible variety with powdery mildew resistance, and is of great significance to cultivation of a soybean powdery mildew resistant soybean variety.

The present invention relates to plant genes involved in regulating flowering, and especially to genes involved in the induction of flowering in response to cold, or vernalization. In particular, the present invention provides the identification, cloning, and characterization of genes involved in vernalization, and specifically of VIP genes, as well as to the proteins encoded by these genes, and to methods of using the VIP genes and proteins. Mutants of VIP genes, where the mutation is a knock-out mutation, confer a vernalization independence, or constitutively vernalized, phenotype in a plant which in the non-mutant form requires vernalization to flower.

A method of manufacturing a reference material for determining incorporation of a genetically modified (GM) plant into a sample or analyzing a mixing ratio from a tissue-cultured cell line that is obtained by incubating tissues of either a GM plant or a non-GM plant, and a method of determining incorporation of a GM plant into a sample and analyzing a mixing ratio using the reference material are provided. The reference material for determining the incorporation of a genetically modified (GM) plant a sample or analyzing a mixing ratio using the tissue-cultured cell lines that are obtained by incubating tissues of the GM plant and the non-GM plant can be useful in producing a countless number of populations having the same genetic traits via the tissue culture. Thus, when a culture capacity of the reference material is increased to a large volume, it is possible to obtain a large volume of the reference material having uniform qualities with no quality variation between batches. Unlike the conventional reference materials manufactured using grain powder, a reference material with 100% purity can be obtained as either a GM or non-GM reference material by verifying the purity of the tissue-cultured cell line. Accordingly, it is possible to provide the uniform and stable supply of a reference material having uniform compositions.