2 results about "Constant region" patented technology

The invention discloses a physiological general CAR-T cell which enables expression of CAR to be controlled by an endogenous promoter and a preparation method and application thereof. A TCR constant region gene is knocked out by utilizing a CRISPR / Cas9 gene editing technique, and the CAR gene is inserted into a TCR constant region precisely by using an AAV-mediated homologous recombination technique. Therefore, the general CAR-T enabling the CAR expression controlled by the endogenous promoter and enabling the expression level of CAR be controlled within a normal physiological range can be obtained. The general CAR-T cell has the characteristics of being allogeneically infused, the expression of CAR is at the physiological level and uniform and consistent, no significant cytokine storm exists, and the cell is safer. The invention also relates to application of the CAR-T cell on anti-tumor aspect.

The invention relates to the technical field of biology, in particular to a polypeptide, a preparation method of the polypeptide, a bispecific antibody and application of the bispecific antibody. The polypeptide is an Fc binding polypeptide, and can be universally, simply and conveniently connected with a monoclonal antibody to prepare a bispecific / multispecific antibody. The polyvalent Fc binding polypeptide PGLU-Fc-III-4C or PASP-Fc-III-4C is prepared by bonding side carboxyl groups of polyglutamic acid (PGLU) or polyaspartic acid (PASP) with Fc-III-4C polypeptide which has general high affinity to an immune globulin (antibody) constant region (Fc), and the polyvalent Fc binding polypeptide can react with various monoclonal antibodies in different proportions in an aqueous solution to obtain a bispecific / multispecific antibody. The preparation method of the bispecific / multispecific antibody has the following advantages: the bispecific / multispecific antibody is prepared by direct incubation in a water environment, the reaction condition is mild, and the activity of the monoclonal antibody can be retained to the greatest extent; and the method is simple, efficient, controllable in monoclonal antibody proportion and high in universality, and has an industrialization prospect.