7 results about "Cytokine" patented technology

The invention relates to a serum-free culture system for efficiently culturing human umbilical cord mesenchymal stem cells in vitro. The serum-free culture system comprises the following components: a basal culture medium a-MEM, cell factors, hormones and proteins, unsaturated fatty acids, antioxidants, energy substances, an amino acid additive, vitamins and metal additives. The serum-free culture system comprises simple and clear components, is free from harms of pathogens, does not have difference between batches, has good repeatability, can obtain plenty of high-quality human umbilical cord mesenchymal stem cells in a short time and has high passage stability, so that the serum-free culture system can be applied to scientific researches and can provide high-purity vibrant cells for cell therapy as a mating system in cell therapy.

The invention discloses a physiological general CAR-T cell which enables expression of CAR to be controlled by an endogenous promoter and a preparation method and application thereof. A TCR constant region gene is knocked out by utilizing a CRISPR / Cas9 gene editing technique, and the CAR gene is inserted into a TCR constant region precisely by using an AAV-mediated homologous recombination technique. Therefore, the general CAR-T enabling the CAR expression controlled by the endogenous promoter and enabling the expression level of CAR be controlled within a normal physiological range can be obtained. The general CAR-T cell has the characteristics of being allogeneically infused, the expression of CAR is at the physiological level and uniform and consistent, no significant cytokine storm exists, and the cell is safer. The invention also relates to application of the CAR-T cell on anti-tumor aspect.

The invention provides a preparation method of human adipose mesenchymal stem cell exosome for treating myocardial infarction, and relates to the technical field of biology. The preparation method ofthe human mesenchymal stem cell exosome for treating myocardial infarction provided by the invention comprises the following steps: culturing human adipose mesenchymal stem cells, and collecting a cell culture solution; and carrying out differential centrifugation on the cell culture solution, collecting the supernatant, carrying out ultracentrifugation on the supernatant to obtain target microspheres, and then filtering, purifying and sterilizing the target microspheres to obtain the human adipose mesenchymal stem cell exosome for treating myocardial infarction. According to the present invention, the advantage is that the human adipose mesenchymal stem cell exosome is prepared by culturing human adipose mesenchymal stem cells, and the human adipose mesenchymal stem cell exosome containsvarious RNAs and cytokines, so that infarcted myocardial cells are effectively repaired, the cardiac function is enhanced, compensatory myocardial load is reduced, and heart failure is relieved; and atreatment medicine or a treatment strategy is provided for myocardial infarction.