6 results about "Mesenchymal stem cell" patented technology

The invention relates to the technical field of cell culture, and in particular to a serum-free culture medium, a preparation method thereof and a culture method of mesenchymal stem cells. The culturemedium consists of the following components: non-essential amino acid, glutamine, hydrocortisone, dexamethasone, polyvinyl alcohol, recombinant human insulin, recombinant human transferrin, a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human Wnt-3a protein, a recombinant human fibronectin, recombinant human laminin, L-glutathione, L-ascorbic acid, beta-mercaptoethanol, ethanolamine, Pluronic F-68, Tween 80, cholesterol, adenine, sodium selenite and a basic culture medium. According to the invention, polyvinyl alcohol can replaceserum albumin in a serum-free culture medium in carrier function, so that proliferation capacity of mesenchymal stem cells is effectively improved. Polyvinyl alcohol has more stable performance thanserum albumin, and the problem that proliferation capacity and passage capacity of stem cells are reduced can be effectively solved.

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

The invention discloses a cell complex used for periodontal tissue repairing, and applications thereof. The cell complex comprises 0.5*10<4> to 5*10<4>/ml human umbilical cord mesenchymal stem cells and 0.5*10<4> to 5*10<4>/ml periodontal ligament mesenchymal stem cells. The cell complex can be used for preparing drugs used for periodontal tissue repairing. The cell complex composed of human umbilical cord mesenchymal stem cells and periodontal ligament mesenchymal stem cells is capable of inhibiting expression of inflammatory factors obviously, and possesses obvious effects on periodontal tissue repairing.

The invention discloses a cell tracer with low toxicity and a high cell uptake rate as well as a preparation method and application of the cell tracer. The cell tracer is a PAA and PEG coated ultra-small superparamagnetic iron oxide nanoparticle. The cell tracer is obtained by synthesizing an ultra-small superparamagnetic iron oxide nanoparticle through a thermal decomposition method and then sequentially coating PAA and PEG. The cell tracer with the low toxicity and the high cell uptake rate is obtained by coating the ultra-small superparamagnetic iron oxide nanoparticles with the PAA and thePEG, and can be used as a cell tracer applied to nuclear magnetic resonance imaging; the cell tracer can be used for efficiently marking human adipose tissue-derived stromal cells without using a transfection reagent, has no obvious toxicity to the human adipose tissue-derived stromal cells and induced pluripotent stem cells, and can be used for tracing the human umbilical cord mesenchymal stem cells transplanted into a brain of a rat.